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Here, we study the roles played by fungal kinases and transcription factors (TFs) in blood-brain barrier (BBB) crossing and brain infection in mice. We use a brain infectivity assay to screen signature-tagged mutagenesis (STM)-based libraries of mutants defective in kinases and TFs, generated in the\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    H99 strain. We also monitor in vivo transcription profiles of kinases and TFs during host infection using NanoString technology. These analyses identify signalling components involved in BBB adhesion and crossing, or survival in the brain parenchyma. The TFs Pdr802, Hob1, and Sre1 are required for infection under all the conditions tested here. Hob1 controls the expression of several factors involved in brain infection, including inositol transporters, a metalloprotease,\n                    <jats:italic>PDR802<\/jats:italic>\n                    , and\n                    <jats:italic>SRE1<\/jats:italic>\n                    . However, Hob1 is dispensable for most cellular functions in\n                    <jats:italic>Cryptococcus deuterogattii<\/jats:italic>\n                    R265, a strain that does not target the brain during infection. Our results indicate that Hob1 is a master regulator of brain infectivity in\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41467-020-15329-2","type":"journal-article","created":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T07:03:40Z","timestamp":1584947020000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Fungal kinases and transcription factors regulating brain infection in Cryptococcus neoformans"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0068-6220","authenticated-orcid":false,"given":"Kyung-Tae","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1917-5455","authenticated-orcid":false,"given":"Joohyeon","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6767-9543","authenticated-orcid":false,"given":"Dong-Gi","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5794-9984","authenticated-orcid":false,"given":"Minjae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyeon","family":"Cha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6265-040X","authenticated-orcid":false,"given":"Yu-Gyeong","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Woo","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areum","family":"Hwangbo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1027-9220","authenticated-orcid":false,"given":"Yelin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang-Jie","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1966-470X","authenticated-orcid":false,"given":"Ying-Lien","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6257-331X","authenticated-orcid":false,"given":"Jong-Seung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6158-7851","authenticated-orcid":false,"given":"Eunji","family":"Cheong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9573-5752","authenticated-orcid":false,"given":"Yong-Sun","family":"Bahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,23]]},"reference":[{"key":"15329_CR1","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1038\/nature10947","volume":"484","author":"MC Fisher","year":"2012","unstructured":"Fisher, M. 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