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Two homologs of O-GlcNAc transferase, SECRET AGENT (SEC) and SPINDLY (SPY), which have O-GlcNAc and O-fucosyl transferase activities, respectively, are essential in\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    but have largely unknown cellular targets. Here we show that AtACINUS is O-GlcNAcylated and O-fucosylated and mediates regulation of transcription, alternative splicing (AS), and developmental transitions. Knocking-out both AtACINUS and its distant paralog AtPININ causes severe growth defects including dwarfism, delayed seed germination and flowering, and abscisic acid (ABA) hypersensitivity. Transcriptomic and protein-DNA\/RNA interaction analyses demonstrate that AtACINUS represses transcription of the flowering repressor\n                    <jats:italic>FLC<\/jats:italic>\n                    and mediates AS of\n                    <jats:italic>ABH1<\/jats:italic>\n                    and\n                    <jats:italic>HAB1<\/jats:italic>\n                    , two negative regulators of ABA signaling. Proteomic analyses show AtACINUS\u2019s O-GlcNAcylation, O-fucosylation, and association with splicing factors, chromatin remodelers, and transcriptional regulators. Some AtACINUS\/AtPININ-dependent AS events are altered in the\n                    <jats:italic>sec<\/jats:italic>\n                    and\n                    <jats:italic>spy<\/jats:italic>\n                    mutants, demonstrating a function of O-glycosylation in regulating alternative RNA splicing.\n                  <\/jats:p>","DOI":"10.1038\/s41467-021-20929-7","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T06:05:48Z","timestamp":1613023548000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Arabidopsis ACINUS is O-glycosylated and regulates transcription and alternative splicing of regulators of reproductive transitions"],"prefix":"10.1038","volume":"12","author":[{"given":"Yang","family":"Bi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9663-3088","authenticated-orcid":false,"given":"Zhiping","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimin","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Shrestha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dasha","family":"Savage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Hartwig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunita","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su Hyun","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4759-2341","authenticated-orcid":false,"given":"Juan A.","family":"Oses-Prieto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy H.","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter H.","family":"Quail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8403-7307","authenticated-orcid":false,"given":"Alma L.","family":"Burlingame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6741-9506","authenticated-orcid":false,"given":"Shou-Ling","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4602-3390","authenticated-orcid":false,"given":"Zhi-Yong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,11]]},"reference":[{"key":"20929_CR1","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.bbagen.2009.07.017","volume":"1800","author":"JA Hanover","year":"2010","unstructured":"Hanover, J. 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