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However, photobleaching typically limits observation times to merely a few seconds, restricting downstream statistical analysis and observation of rare biological events. Here, we overcome this inherent limitation\n                    <jats:italic>via<\/jats:italic>\n                    continuous fluorophore exchange using DNA-PAINT, where fluorescently-labeled oligonucleotides reversibly bind to a single-stranded DNA handle attached to the target molecule. Such versatile and facile labeling allows uninterrupted monitoring of single molecules for extended durations. We demonstrate the power of our approach by observing DNA origami on membranes for tens of minutes, providing perspectives for investigating cellular processes on physiologically relevant timescales.\n                  <\/jats:p>","DOI":"10.1038\/s41467-021-24223-4","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T06:07:53Z","timestamp":1626847673000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Tracking single particles for hours via continuous DNA-mediated fluorophore exchange"],"prefix":"10.1038","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8571-8633","authenticated-orcid":false,"given":"Florian","family":"Stehr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1335-1120","authenticated-orcid":false,"given":"Johannes","family":"Stein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7110-3494","authenticated-orcid":false,"given":"Julian","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4383-887X","authenticated-orcid":false,"given":"Christian","family":"Niederauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4607-3312","authenticated-orcid":false,"given":"Ralf","family":"Jungmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9106-9406","authenticated-orcid":false,"given":"Kristina","family":"Ganzinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6106-4847","authenticated-orcid":false,"given":"Petra","family":"Schwille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,21]]},"reference":[{"key":"24223_CR1","doi-asserted-by":"publisher","first-page":"124601","DOI":"10.1088\/0034-4885\/78\/12\/124601","volume":"78","author":"C Manzo","year":"2015","unstructured":"Manzo, C. & Garcia-Parajo, M. 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