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This study investigates whether skeletal stem\/progenitor cells are resident in the cranial sutures. Prospective isolation by FACS identifies this population with a significant difference in spatio-temporal representation between fusing versus patent sutures. Transcriptomic analysis highlights a distinct signature in cells\u00a0derived from the physiological closing PF suture, and scRNA sequencing identifies transcriptional heterogeneity among sutures. Wnt-signaling activation increases skeletal stem\/progenitor cells in sutures, whereas its inhibition decreases. Crossing\n                    <jats:italic>Axin2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>LacZ\/+<\/jats:italic>\n                    <\/jats:sup>\n                    mouse, endowing enhanced Wnt activation, to a\n                    <jats:italic>Twist1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>+\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mouse model of coronal craniosynostosis enriches skeletal stem\/progenitor cells in sutures restoring patency. Co-transplantation of these cells with Wnt3a prevents resynostosis following suturectomy in\n                    <jats:italic>Twist1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>+\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice. Our study reveals that decrease and\/or imbalance of skeletal stem\/progenitor cells representation within sutures may underlie craniosynostosis. These findings have translational implications toward therapeutic approaches for craniosynostosis.\n                  <\/jats:p>","DOI":"10.1038\/s41467-021-24801-6","type":"journal-article","created":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T06:04:43Z","timestamp":1627625083000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Skeletal stem and progenitor cells maintain cranial suture patency and prevent craniosynostosis"],"prefix":"10.1038","volume":"12","author":[{"given":"Siddharth","family":"Menon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6107-6656","authenticated-orcid":false,"given":"Ankit","family":"Salhotra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siny","family":"Shailendra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Tevlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan C.","family":"Ransom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Januszyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6570-7574","authenticated-orcid":false,"given":"Charles K. 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