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However, AR can be temporally induced, and the possibility that this causes transient, non-inherited CS, has not been addressed. Mutational acquisition of ciprofloxacin resistance leads to robust CS to tobramycin in pre-existing antibiotic-resistant mutants of\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . Further, the strength of this phenotype is higher when\n                    <jats:italic>nfxB<\/jats:italic>\n                    mutants, over-producing the efflux pump MexCD-OprJ, are selected. Here, we induce transient\n                    <jats:italic>nfxB<\/jats:italic>\n                    -mediated ciprofloxacin resistance by using the antiseptic dequalinium chloride. Notably, non-inherited induction of AR renders transient tobramycin CS in the analyzed antibiotic-resistant mutants and clinical isolates, including tobramycin-resistant isolates. Further, by combining tobramycin with dequalinium chloride we drive these strains to extinction. Our results support that transient CS could allow the design of new evolutionary strategies to tackle antibiotic-resistant infections, avoiding the acquisition of AR mutations on which inherited CS depends.\n                  <\/jats:p>","DOI":"10.1038\/s41467-023-37357-4","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T06:03:56Z","timestamp":1680156236000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Tackling antibiotic resistance by inducing transient and robust collateral sensitivity"],"prefix":"10.1038","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5822-4996","authenticated-orcid":false,"given":"Sara","family":"Hernando-Amado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8281-7900","authenticated-orcid":false,"given":"Pablo","family":"Laborda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8813-7607","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"37357_CR1","doi-asserted-by":"publisher","first-page":"2475067","DOI":"10.1155\/2016\/2475067","volume":"2016","author":"S Santajit","year":"2016","unstructured":"Santajit, S. & Indrawattana, N. 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