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Here we describe a transgenic system for recreating CI in the major arbovirus vector\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    using CI factor (\n                    <jats:italic>cif<\/jats:italic>\n                    ) genes from\n                    <jats:italic>w<\/jats:italic>\n                    AlbB, a\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    strain currently being deployed to reduce dengue transmission. CI-like sterility is induced when\n                    <jats:italic>cifA<\/jats:italic>\n                    and\n                    <jats:italic>cifB<\/jats:italic>\n                    are co-expressed in testes; this sterility is rescued by maternal\n                    <jats:italic>cifA<\/jats:italic>\n                    expression, thereby reproducing the pattern of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    -induced CI. Expression of\n                    <jats:italic>cifB<\/jats:italic>\n                    alone is associated with extensive DNA damage and disrupted spermatogenesis. The strength of rescue by maternal\n                    <jats:italic>cifA<\/jats:italic>\n                    expression is dependent on the comparative levels of\n                    <jats:italic>cifA\/cifB<\/jats:italic>\n                    expression in males. These findings are consistent with CifB acting as a toxin and CifA as an antitoxin, with CifA attenuating CifB toxicity in both the male germline and in developing embryos. These findings provide important insights into the interactions between\n                    <jats:italic>cif<\/jats:italic>\n                    genes and their mechanism of activity and provide a foundation for the building of a\n                    <jats:italic>cif<\/jats:italic>\n                    gene-based drive system in\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-45238-7","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T10:02:43Z","timestamp":1706522563000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti"],"prefix":"10.1038","volume":"15","author":[{"given":"Cameron J.","family":"McNamara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas H.","family":"Ant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4006-7034","authenticated-orcid":false,"given":"Tim","family":"Harvey-Samuel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"White-Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8210-2921","authenticated-orcid":false,"given":"Julien","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2916-3802","authenticated-orcid":false,"given":"Luke","family":"Alphey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2678-1832","authenticated-orcid":false,"given":"Steven P.","family":"Sinkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"45238_CR1","doi-asserted-by":"publisher","first-page":"17007","DOI":"10.1038\/nmicrobiol.2017.7","volume":"2","author":"JF Beckmann","year":"2017","unstructured":"Beckmann, J. 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