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However, these findings are mostly based on m\n                    <jats:sup>6<\/jats:sup>\n                    A-Seq, an antibody-dependent technique with a high rate of false positives. Here, we address the presence of m\n                    <jats:sup>6<\/jats:sup>\n                    A in CHIKV and DENV RNAs. For this, we combine m\n                    <jats:sup>6<\/jats:sup>\n                    A-Seq and the antibody-independent SELECT and nanopore direct RNA sequencing techniques with functional, molecular, and mutagenesis studies. Following this comprehensive analysis, we find no evidence of m\n                    <jats:sup>6<\/jats:sup>\n                    A modification in CHIKV or DENV transcripts. Furthermore, depletion of key components of the host m\n                    <jats:sup>6<\/jats:sup>\n                    A machinery does not affect CHIKV or DENV infection. Moreover, CHIKV or DENV infection has no effect on the m\n                    <jats:sup>6<\/jats:sup>\n                    A machinery\u2019s localization. Our results challenge the prevailing notion that m\n                    <jats:sup>6<\/jats:sup>\n                    A modification is a general feature of cytoplasmic RNA viruses and underscore the importance of validating RNA modifications with orthogonal approaches.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-46278-9","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T07:01:52Z","timestamp":1710140512000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["N6-methyladenosine modification is not a general trait of viral RNA genomes"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0956-7164","authenticated-orcid":false,"given":"Belinda","family":"Baquero-P\u00e9rez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8486-7362","authenticated-orcid":false,"given":"Ivaylo D.","family":"Yonchev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3836-0293","authenticated-orcid":false,"given":"Anna","family":"Delgado-Tejedor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebeca","family":"Medina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireia","family":"Puig-Torrents","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5038-0190","authenticated-orcid":false,"given":"Ian","family":"Sudbery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8663-4586","authenticated-orcid":false,"given":"Oguzhan","family":"Begik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3073-258X","authenticated-orcid":false,"given":"Stuart A.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9367-6311","authenticated-orcid":false,"given":"Eva Maria","family":"Novoa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9013-0751","authenticated-orcid":false,"given":"Juana","family":"D\u00edez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,11]]},"reference":[{"key":"46278_CR1","doi-asserted-by":"publisher","first-page":"e105977","DOI":"10.15252\/embj.2020105977","volume":"40","author":"PC He","year":"2021","unstructured":"He, P. 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