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Aberrant glycosylation is often correlated with inflammation and pathology such as COPD, cancer, and Crohn\u2019s disease. The inherent complexity of glycans and the diversity in the\n                    <jats:italic>O<\/jats:italic>\n                    -core structure constitute fundamental challenges for the analysis of mucin-type\n                    <jats:italic>O<\/jats:italic>\n                    -glycans. Due to coexistence of multiple isomers, multidimensional workflows such as LC-MS are required. To separate the highly polar carbohydrates, porous graphitized carbon is often used as a stationary phase. However, LC-MS workflows are time-consuming and lack reproducibility. Here we present a rapid alternative for separating and identifying\n                    <jats:italic>O<\/jats:italic>\n                    -glycans released from mucins based on trapped ion mobility mass spectrometry. Compared to established LC-MS, the acquisition time is reduced from an hour to two minutes. To test the validity, the developed workflow was applied to sputum samples from cystic fibrosis patients to map\n                    <jats:italic>O<\/jats:italic>\n                    -glycosylation features associated with disease.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-46825-4","type":"journal-article","created":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T06:01:55Z","timestamp":1711173715000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Ion mobility-tandem mass spectrometry of mucin-type O-glycans"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7354-3108","authenticated-orcid":false,"given":"Le\u00efla","family":"Bechtella","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0229-102X","authenticated-orcid":false,"given":"Jin","family":"Chunsheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0437-1049","authenticated-orcid":false,"given":"Kerstin","family":"Fentker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcney R.","family":"Ert\u00fcrk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9267-3940","authenticated-orcid":false,"given":"Marc","family":"Safferthal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9802-2528","authenticated-orcid":false,"given":"\u0141ukasz","family":"Polewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4001-2324","authenticated-orcid":false,"given":"Michael","family":"G\u00f6tze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1666-8924","authenticated-orcid":false,"given":"Simon Y.","family":"Graeber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebl M.","family":"Vos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0594-226X","authenticated-orcid":false,"given":"Weston B.","family":"Struwe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4057-2199","authenticated-orcid":false,"given":"Marcus A.","family":"Mall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2245-528X","authenticated-orcid":false,"given":"Philipp","family":"Mertins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niclas G.","family":"Karlsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8054-4718","authenticated-orcid":false,"given":"Kevin","family":"Pagel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,23]]},"reference":[{"key":"46825_CR1","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.cocis.2005.11.001","volume":"11","author":"R Bansil","year":"2006","unstructured":"Bansil, R. & Turner, B. 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