{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T23:31:51Z","timestamp":1793748711178,"version":"4.6.1"},"reference-count":72,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000060","name":"U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI104946"],"award-info":[{"award-number":["R01AI104946"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902089"],"award-info":[{"award-number":["81902089"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902089"],"award-info":[{"award-number":["81902089"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Commun"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In malaria parasites, the regulation of mRNA translation, storage and degradation during development and life-stage transitions remains largely unknown. Here, we functionally characterized the DEAD-box RNA helicase PfDOZI in\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    . Disruption of\n                    <jats:italic>pfdozi<\/jats:italic>\n                    enhanced asexual proliferation but reduced sexual commitment and impaired gametocyte development. By quantitative transcriptomics, we show that PfDOZI is involved in the regulation of invasion-related genes and sexual stage-specific genes during different developmental stages. PfDOZI predominantly participates in processing body-like mRNPs in schizonts but germ cell granule-like mRNPs in gametocytes to impose opposing actions of degradation and protection on different mRNA targets. We further show the formation of stress granule-like mRNPs during nutritional deprivation, highlighting an essential role of PfDOZI-associated mRNPs in stress response. We demonstrate that PfDOZI participates in distinct mRNPs to maintain mRNA homeostasis in response to life-stage transition and environmental changes by differentially executing post-transcriptional regulation on the target mRNAs.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-48140-4","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T14:02:06Z","timestamp":1714744926000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The DEAD-box RNA helicase PfDOZI imposes opposing actions on RNA metabolism in Plasmodium falciparum"],"prefix":"10.1038","volume":"15","author":[{"given":"Hui","family":"Min","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7582-3824","authenticated-orcid":false,"given":"Chengqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junling","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachasak","family":"Boonhok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5942-9231","authenticated-orcid":false,"given":"Azhar","family":"Muneer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Awtum M.","family":"Brashear","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen M.","family":"Minns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swamy Rakesh","family":"Adapa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rays H. Y.","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaming","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1799-3726","authenticated-orcid":false,"given":"Scott E.","family":"Lindner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9374-6749","authenticated-orcid":false,"given":"Jun","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8338-1974","authenticated-orcid":false,"given":"Liwang","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,3]]},"reference":[{"key":"48140_CR1","unstructured":"WHO. World Malaria Report 2023. (WHO, Geneva, 2023)."},{"key":"48140_CR2","doi-asserted-by":"publisher","first-page":"e1002451","DOI":"10.1371\/journal.pmed.1002451","volume":"14","author":"The malERA Refresh Consultative Panel on Basic Science and Enabling Technologies.","year":"2017","unstructured":"The malERA Refresh Consultative Panel on Basic Science and Enabling Technologies. malERA: an updated research agenda for basic science and enabling technologies in malaria elimination and eradication. PLoS Med. 14, e1002451 (2017).","journal-title":"PLoS Med."},{"key":"48140_CR3","doi-asserted-by":"crossref","unstructured":"Cortopassi, W. A., Celmar Costa Franca, T. & Krettli, A. U. A systems biology approach to antimalarial drug discovery. Expert Opin. Drug Discov. 13, 617\u2013626 (2018).","DOI":"10.1080\/17460441.2018.1471056"},{"key":"48140_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/nyas.12573","volume":"1342","author":"L Cui","year":"2015","unstructured":"Cui, L., Lindner, S. & Miao, J. Translational regulation during stage transitions in malaria parasites. Ann. N. Y. Acad. Sci. 1342, 1\u20139 (2015).","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"48140_CR5","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1002\/wrna.1365","volume":"7","author":"SS Vembar","year":"2016","unstructured":"Vembar, S. S., Droll, D. & Scherf, A. Translational regulation in blood stages of the malaria parasite Plasmodium spp.: systems-wide studies pave the way. Wiley Interdiscip. Rev. RNA 7, 772\u2013792 (2016).","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"48140_CR6","doi-asserted-by":"publisher","first-page":"M110 006411","DOI":"10.1074\/mcp.M110.006411","volume":"10","author":"BJ Foth","year":"2011","unstructured":"Foth, B. J. et al. Quantitative time-course profiling of parasite and host cell proteins in the human malaria parasite Plasmodium falciparum. Mol. Cell. Proteom. 10, M110 006411 (2011).","journal-title":"Mol. Cell. Proteom."},{"key":"48140_CR7","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-12-r177","volume":"9","author":"BJ Foth","year":"2008","unstructured":"Foth, B. J., Zhang, N., Mok, S., Preiser, P. R. & Bozdech, Z. Quantitative protein expression profiling reveals extensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites. Genome Biol. 9, R177 (2008).","journal-title":"Genome Biol."},{"key":"48140_CR8","doi-asserted-by":"publisher","first-page":"2308","DOI":"10.1101\/gr.2523904","volume":"14","author":"KG Le Roch","year":"2004","unstructured":"Le Roch, K. G. et al. Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle. Genome Res. 14, 2308\u20132318 (2004).","journal-title":"Genome Res."},{"key":"48140_CR9","doi-asserted-by":"publisher","first-page":"e1008095","DOI":"10.1371\/journal.ppat.1008095","volume":"15","author":"KT Rios","year":"2019","unstructured":"Rios, K. T. & Lindner, S. E. Protein-RNA interactions important for Plasmodium transmission. PLoS Pathog. 15, e1008095 (2019).","journal-title":"PLoS Pathog."},{"key":"48140_CR10","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1111\/mmi.13762","volume":"106","author":"EE Mu\u00f1oz","year":"2017","unstructured":"Mu\u00f1oz, E. E. et al. ALBA4 modulates its stage-specific interactions and specific mRNA fates during Plasmodium yoelii growth and transmission. Mol. Microbiol. 106, 266\u2013284 (2017).","journal-title":"Mol. Microbiol."},{"key":"48140_CR11","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0771-5","volume":"16","author":"SS Vembar","year":"2015","unstructured":"Vembar, S. S., Macpherson, C. R., Sismeiro, O., Coppee, J. Y. & Scherf, A. The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages. Genome Biol. 16, 212 (2015).","journal-title":"Genome Biol."},{"key":"48140_CR12","doi-asserted-by":"publisher","first-page":"a012286","DOI":"10.1101\/cshperspect.a012286","volume":"4","author":"CJ Decker","year":"2012","unstructured":"Decker, C. J. & Parker, R. P-bodies and stress granules: possible roles in the control of translation and mRNA degradation. Cold Spring Harb. Perspect. Biol. 4, a012286 (2012).","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"48140_CR13","doi-asserted-by":"publisher","first-page":"932","DOI":"10.1016\/j.molcel.2009.11.020","volume":"36","author":"JR Buchan","year":"2009","unstructured":"Buchan, J. R. & Parker, R. Eukaryotic stress granules: the ins and outs of translation. Mol. Cell 36, 932\u2013941 (2009).","journal-title":"Mol. Cell"},{"key":"48140_CR14","doi-asserted-by":"publisher","first-page":"a032813","DOI":"10.1101\/cshperspect.a032813","volume":"11","author":"P Ivanov","year":"2019","unstructured":"Ivanov, P., Kedersha, N. & Anderson, P. Stress granules and processing bodies in translational control. Cold Spring Harb. Perspect. Biol. 11, a032813 (2019).","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"48140_CR15","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1038\/nrm2694","volume":"10","author":"P Anderson","year":"2009","unstructured":"Anderson, P. & Kedersha, N. RNA granules: post-transcriptional and epigenetic modulators of gene expression. Nat. Rev. Mol. Cell Biol. 10, 430\u2013436 (2009).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"48140_CR16","doi-asserted-by":"crossref","unstructured":"Voronina, E., Seydoux, G., Sassone-Corsi, P. & Nagamori, I. RNA granules in germ cells. Cold Spring Harb. Perspect. Biol. 3, a002774 (2011).","DOI":"10.1101\/cshperspect.a002774"},{"key":"48140_CR17","doi-asserted-by":"publisher","first-page":"e1642","DOI":"10.1002\/wrna.1642","volume":"12","author":"N Mukherjee","year":"2021","unstructured":"Mukherjee, N. & Mukherjee, C. Germ cell ribonucleoprotein granules in different clades of life: From insects to mammals. Wiley Interdiscip. Rev. RNA 12, e1642 (2021).","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"48140_CR18","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-1014-0","volume":"17","author":"EM Bunnik","year":"2016","unstructured":"Bunnik, E. M. et al. The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. Genome Biol. 17, 147 (2016).","journal-title":"Genome Biol."},{"key":"48140_CR19","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1016\/j.bbagrm.2013.03.006","volume":"1829","author":"V Presnyak","year":"2013","unstructured":"Presnyak, V. & Coller, J. The DHH1\/RCKp54 family of helicases: an ancient family of proteins that promote translational silencing. Biochim. Biophys. Acta 1829, 817\u2013823 (2013).","journal-title":"Biochim. Biophys. Acta"},{"key":"48140_CR20","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1002\/wrna.1237","volume":"5","author":"DH Ostareck","year":"2014","unstructured":"Ostareck, D. H., Naarmann-de Vries, I. S. & Ostareck-Lederer, A. DDX6 and its orthologs as modulators of cellular and viral RNA expression. Wiley Interdiscip. Rev. RNA 5, 659\u2013678 (2014).","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"48140_CR21","doi-asserted-by":"publisher","first-page":"3082","DOI":"10.1093\/nar\/gkl409","volume":"34","author":"A Weston","year":"2006","unstructured":"Weston, A. & Sommerville, J. Xp54 and related (DDX6-like) RNA helicases: roles in messenger RNP assembly, translation regulation and RNA degradation. Nucleic Acids Res. 34, 3082\u20133094 (2006).","journal-title":"Nucleic Acids Res."},{"key":"48140_CR22","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.tcb.2008.11.001","volume":"19","author":"P Rajyaguru","year":"2009","unstructured":"Rajyaguru, P. & Parker, R. CGH-1 and the control of maternal mRNAs. Trends Cell Biol. 19, 24\u201328 (2009).","journal-title":"Trends Cell Biol."},{"key":"48140_CR23","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1126\/science.1125129","volume":"313","author":"GR Mair","year":"2006","unstructured":"Mair, G. R. et al. Regulation of sexual development of Plasmodium by translational repression. Science 313, 667\u2013669 (2006).","journal-title":"Science"},{"key":"48140_CR24","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1016\/j.cell.2005.07.012","volume":"122","author":"J Coller","year":"2005","unstructured":"Coller, J. & Parker, R. General translational repression by activators of mRNA decapping. Cell 122, 875\u2013886 (2005).","journal-title":"Cell"},{"key":"48140_CR25","doi-asserted-by":"publisher","first-page":"2558","DOI":"10.1101\/gad.443107","volume":"21","author":"A Eulalio","year":"2007","unstructured":"Eulalio, A. et al. Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing. Genes Dev. 21, 2558\u20132570 (2007).","journal-title":"Genes Dev."},{"key":"48140_CR26","doi-asserted-by":"publisher","first-page":"e1000767","DOI":"10.1371\/journal.ppat.1000767","volume":"6","author":"GR Mair","year":"2010","unstructured":"Mair, G. R. et al. Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development. PLoS Pathog. 6, e1000767 (2010).","journal-title":"PLoS Pathog."},{"key":"48140_CR27","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0493-0","volume":"15","author":"A Guerreiro","year":"2014","unstructured":"Guerreiro, A. et al. Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte. Genome Biol. 15, 493 (2014).","journal-title":"Genome Biol."},{"key":"48140_CR28","doi-asserted-by":"publisher","first-page":"e14470","DOI":"10.1371\/journal.pone.0014470","volume":"5","author":"S Schwank","year":"2010","unstructured":"Schwank, S., Sutherland, C. J. & Drakeley, C. J. Promiscuous expression of alpha-tubulin II in maturing male and female Plasmodium falciparum gametocytes. PLoS ONE 5, e14470 (2010).","journal-title":"PLoS ONE"},{"key":"48140_CR29","doi-asserted-by":"publisher","first-page":"6087","DOI":"10.1093\/nar\/gkw536","volume":"44","author":"E Lasonder","year":"2016","unstructured":"Lasonder, E. et al. Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression. Nucleic Acids Res. 44, 6087\u20136101 (2016).","journal-title":"Nucleic Acids Res."},{"key":"48140_CR30","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1074\/mcp.M116.061804","volume":"16","author":"J Miao","year":"2017","unstructured":"Miao, J. et al. Sex-specific biology of the human malaria parasite revealed from the proteomes of mature male and female gametocytes. Mol. Cell. Proteom. 16, 537\u2013551 (2017).","journal-title":"Mol. Cell. Proteom."},{"key":"48140_CR31","doi-asserted-by":"publisher","first-page":"3066","DOI":"10.1093\/nar\/gkr1215","volume":"40","author":"A Chene","year":"2012","unstructured":"Chene, A. et al. PfAlbas constitute a new eukaryotic DNA\/RNA-binding protein family in malaria parasites. Nucleic Acids Res. 40, 3066\u20133077 (2012).","journal-title":"Nucleic Acids Res."},{"key":"48140_CR32","doi-asserted-by":"publisher","first-page":"e00435","DOI":"10.1128\/mSphere.00435-17","volume":"3","author":"AM Minns","year":"2018","unstructured":"Minns, A. M., Hart, K. J., Subramanian, S., Hafenstein, S. & Lindner, S. E. Nuclear, cytosolic, and surface-localized poly(a)-binding proteins of Plasmodium yoelii. mSphere 3, e00435\u201300417 (2018).","journal-title":"mSphere"},{"key":"48140_CR33","doi-asserted-by":"publisher","first-page":"e1006659","DOI":"10.1371\/journal.ppat.1006659","volume":"13","author":"M Parkyn Schneider","year":"2017","unstructured":"Parkyn Schneider, M. et al. Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development. PLoS Pathog. 13, e1006659 (2017).","journal-title":"PLoS Pathog."},{"key":"48140_CR34","doi-asserted-by":"publisher","first-page":"7825","DOI":"10.1093\/nar\/gkx464","volume":"45","author":"XM Lu","year":"2017","unstructured":"Lu, X. M. et al. Nascent RNA sequencing reveals mechanisms of gene regulation in the human malaria parasite Plasmodium falciparum. Nucleic Acids Res. 45, 7825\u20137840 (2017).","journal-title":"Nucleic Acids Res."},{"key":"48140_CR35","doi-asserted-by":"publisher","first-page":"3007","DOI":"10.1002\/1873-3468.13183","volume":"592","author":"CL Winata","year":"2018","unstructured":"Winata, C. L. & Korzh, V. The translational regulation of maternal mRNAs in time and space. FEBS Lett. 592, 3007\u20133023 (2018).","journal-title":"FEBS Lett."},{"key":"48140_CR36","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1083\/jcb.200807043","volume":"183","author":"JR Buchan","year":"2008","unstructured":"Buchan, J. R., Muhlrad, D. & Parker, R. P bodies promote stress granule assembly in Saccharomyces cerevisiae. J. Cell Biol. 183, 441\u2013455 (2008).","journal-title":"J. Cell Biol."},{"key":"48140_CR37","doi-asserted-by":"publisher","first-page":"3536","DOI":"10.1002\/1873-3468.12854","volume":"591","author":"H DeHaan","year":"2017","unstructured":"DeHaan, H. et al. An in vivo proteomic analysis of the Me31B interactome in Drosophila germ granules. FEBS Lett. 591, 3536\u20133547 (2017).","journal-title":"FEBS Lett."},{"key":"48140_CR38","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/j.cell.2015.12.038","volume":"164","author":"S Jain","year":"2016","unstructured":"Jain, S. et al. ATPase-modulated stress granules contain a diverse proteome and substructure. Cell 164, 487\u2013498 (2016).","journal-title":"Cell"},{"key":"48140_CR39","doi-asserted-by":"crossref","unstructured":"Reineke, L. C., Cheema, S. A., Dubrulle, J. & Neilson, J. R. Chronic starvation induces noncanonical pro-death stress granules. J. Cell. Sci. 131, jcs220244 (2018).","DOI":"10.1101\/317529"},{"key":"48140_CR40","doi-asserted-by":"publisher","first-page":"2424","DOI":"10.1021\/acs.biochem.7b01162","volume":"57","author":"Y Luo","year":"2018","unstructured":"Luo, Y., Na, Z. & Slavoff, S. A. P-bodies: composition, properties, and functions. Biochemistry 57, 2424\u20132431 (2018).","journal-title":"Biochemistry"},{"key":"48140_CR41","doi-asserted-by":"publisher","first-page":"2342","DOI":"10.1080\/15476286.2021.1910403","volume":"18","author":"G Poornima","year":"2021","unstructured":"Poornima, G. et al. RGG-motif containing mRNA export factor Gbp2 acts as a translation repressor. RNA Biol. 18, 2342\u20132353 (2021).","journal-title":"RNA Biol."},{"key":"48140_CR42","first-page":"2053","volume":"125","author":"MK Dearnley","year":"2012","unstructured":"Dearnley, M. K. et al. Origin, composition, organization and function of the inner membrane complex of Plasmodium falciparum gametocytes. J. Cell Sci. 125, 2053\u20132063 (2012).","journal-title":"J. Cell Sci."},{"key":"48140_CR43","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10727","volume":"7","author":"SM Ganesan","year":"2016","unstructured":"Ganesan, S. M., Falla, A., Goldfless, S. J., Nasamu, A. S. & Niles, J. C. Synthetic RNA-protein modules integrated with native translation mechanisms to control gene expression in malaria parasites. Nat. Commun. 7, 10727 (2016).","journal-title":"Nat. Commun."},{"key":"48140_CR44","doi-asserted-by":"publisher","first-page":"e10006","DOI":"10.1371\/journal.pone.0010006","volume":"5","author":"KD Swisher","year":"2010","unstructured":"Swisher, K. D. & Parker, R. Localization to, and effects of Pbp1, Pbp4, Lsm12, Dhh1, and Pab1 on stress granules in Saccharomyces cerevisiae. PLoS ONE 5, e10006 (2010).","journal-title":"PLoS ONE"},{"key":"48140_CR45","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1083\/jcb.200502088","volume":"169","author":"N Kedersha","year":"2005","unstructured":"Kedersha, N. et al. Stress granules and processing bodies are dynamically linked sites of mRNP remodeling. J. Cell Biol. 169, 871\u2013884 (2005).","journal-title":"J. Cell Biol."},{"key":"48140_CR46","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1111\/j.1440-169X.2011.01314.x","volume":"54","author":"Y Kato","year":"2012","unstructured":"Kato, Y. & Nakamura, A. Roles of cytoplasmic RNP granules in intracellular RNA localization and translational control in the Drosophila oocyte. Dev. Growth Differ. 54, 19\u201331 (2012).","journal-title":"Dev. Growth Differ."},{"key":"48140_CR47","doi-asserted-by":"publisher","first-page":"1707","DOI":"10.1007\/s00018-018-2750-y","volume":"75","author":"C Liu","year":"2018","unstructured":"Liu, C., Ma, Y., Shang, Y., Huo, R. & Li, W. Post-translational regulation of the maternal-to-zygotic transition. Cell. Mol. Life Sci. 75, 1707\u20131722 (2018).","journal-title":"Cell. Mol. Life Sci."},{"key":"48140_CR48","doi-asserted-by":"publisher","first-page":"2592","DOI":"10.1091\/mbc.e06-12-1149","volume":"18","author":"M Brengues","year":"2007","unstructured":"Brengues, M. & Parker, R. Accumulation of polyadenylated mRNA, Pab1p, eIF4E, and eIF4G with P-bodies in Saccharomyces cerevisiae. Mol. Biol. Cell 18, 2592\u20132602 (2007).","journal-title":"Mol. Biol. Cell"},{"key":"48140_CR49","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.molcel.2017.09.003","volume":"68","author":"A Hubstenberger","year":"2017","unstructured":"Hubstenberger, A. et al. P-Body purification reveals the condensation of repressed mRNA regulons. Mol. Cell 68, 144\u2013157 e145 (2017).","journal-title":"Mol. Cell"},{"key":"48140_CR50","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-2092-1","volume":"16","author":"BP Reddy","year":"2015","unstructured":"Reddy, B. P. et al. A bioinformatic survey of RNA-binding proteins in Plasmodium. BMC Genomics 16, 890 (2015).","journal-title":"BMC Genomics"},{"key":"48140_CR51","doi-asserted-by":"publisher","first-page":"4205","DOI":"10.1091\/mbc.e11-06-0511","volume":"22","author":"I Subota","year":"2011","unstructured":"Subota, I. et al. ALBA proteins are stage regulated during trypanosome development in the tsetse fly and participate in differentiation. Mol. Biol. Cell 22, 4205\u20134219 (2011).","journal-title":"Mol. Biol. Cell"},{"key":"48140_CR52","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1016\/j.molcel.2019.09.014","volume":"76","author":"JY Youn","year":"2019","unstructured":"Youn, J. Y. et al. Properties of stress granule and P-body proteomes. Mol. Cell 76, 286\u2013294 (2019).","journal-title":"Mol. Cell"},{"key":"48140_CR53","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1126\/science.1115791","volume":"310","author":"M Brengues","year":"2005","unstructured":"Brengues, M., Teixeira, D. & Parker, R. Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies. Science 310, 486\u2013489 (2005).","journal-title":"Science"},{"key":"48140_CR54","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.tibs.2007.12.003","volume":"33","author":"P Anderson","year":"2008","unstructured":"Anderson, P. & Kedersha, N. Stress granules: the Tao of RNA triage. Trends Biochem. Sci. 33, 141\u2013150 (2008).","journal-title":"Trends Biochem. Sci."},{"key":"48140_CR55","doi-asserted-by":"publisher","first-page":"1431","DOI":"10.1083\/jcb.147.7.1431","volume":"147","author":"NL Kedersha","year":"1999","unstructured":"Kedersha, N. L., Gupta, M., Li, W., Miller, I. & Anderson, P. RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules. J. Cell Biol. 147, 1431\u20131442 (1999).","journal-title":"J. Cell Biol."},{"key":"48140_CR56","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1242\/jcs.199240","volume":"130","author":"A Aulas","year":"2017","unstructured":"Aulas, A. et al. Stress-specific differences in assembly and composition of stress granules and related foci. J. Cell Sci. 130, 927\u2013937 (2017).","journal-title":"J. Cell Sci."},{"key":"48140_CR57","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1016\/j.chom.2017.11.005","volume":"22","author":"M Zhang","year":"2017","unstructured":"Zhang, M. et al. Inhibiting the Plasmodium eIF2alpha kinase PK4 prevents artemisinin-induced latency. Cell Host Microbe 22, 766\u2013776 e764 (2017).","journal-title":"Cell Host Microbe"},{"key":"48140_CR58","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06221-1","volume":"9","author":"JL Bridgford","year":"2018","unstructured":"Bridgford, J. L. et al. Artemisinin kills malaria parasites by damaging proteins and inhibiting the proteasome. Nat. Commun. 9, 3801 (2018).","journal-title":"Nat. Commun."},{"key":"48140_CR59","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24814-1","volume":"12","author":"M Zhang","year":"2021","unstructured":"Zhang, M. et al. The apicoplast link to fever-survival and artemisinin-resistance in the malaria parasite. Nat. Commun. 12, 4563 (2021).","journal-title":"Nat. Commun."},{"key":"48140_CR60","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.molbiopara.2007.04.008","volume":"154","author":"QL Fivelman","year":"2007","unstructured":"Fivelman, Q. L. et al. Improved synchronous production of Plasmodium falciparum gametocytes in vitro. Mol. Biochem. Parasitol. 154, 119\u2013123 (2007).","journal-title":"Mol. Biochem. Parasitol."},{"key":"48140_CR61","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1016\/j.exppara.2013.09.010","volume":"135","author":"J Miao","year":"2013","unstructured":"Miao, J. et al. Plasmodium falciparum: generation of pure gametocyte culture by heparin treatment. Exp. Parasitol. 135, 541\u2013545 (2013).","journal-title":"Exp. Parasitol."},{"key":"48140_CR62","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/978-1-62703-026-7_6","volume":"923","author":"M Rug","year":"2013","unstructured":"Rug, M. & Maier, A. G. Transfection of Plasmodium falciparum. Methods Mol. Biol. 923, 75\u201398 (2013).","journal-title":"Methods Mol. Biol."},{"key":"48140_CR63","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1242\/jcs.059824","volume":"123","author":"J Miao","year":"2010","unstructured":"Miao, J. et al. The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. J. Cell Sci. 123, 1039\u20131049 (2010).","journal-title":"J. Cell Sci."},{"key":"48140_CR64","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1111\/j.1365-2958.2010.07371.x","volume":"78","author":"J Miao","year":"2010","unstructured":"Miao, J. et al. The MYST family histone acetyltransferase regulates gene expression and cell cycle in malaria parasite Plasmodium falciparum. Mol. Microbiol. 78, 883\u2013902 (2010).","journal-title":"Mol. Microbiol."},{"key":"48140_CR65","doi-asserted-by":"publisher","first-page":"e1000746","DOI":"10.1371\/journal.ppat.1000746","volume":"6","author":"S Singh","year":"2010","unstructured":"Singh, S., Alam, M. M., Pal-Bhowmick, I., Brzostowski, J. A. & Chitnis, C. E. Distinct external signals trigger sequential release of apical organelles during erythrocyte invasion by malaria parasites. PLoS Pathog. 6, e1000746 (2010).","journal-title":"PLoS Pathog."},{"key":"48140_CR66","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ijpara.2012.11.002","volume":"43","author":"MJ Boyle","year":"2013","unstructured":"Boyle, M. J., Wilson, D. W. & Beeson, J. G. New approaches to studying Plasmodium falciparum merozoite invasion and insights into invasion biology. Int. J. Parasitol. 43, 1\u201310 (2013).","journal-title":"Int. J. Parasitol."},{"key":"48140_CR67","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.3317","volume":"12","author":"D Kim","year":"2015","unstructured":"Kim, D., Langmead, B. & Salzberg, S. L. HISAT: a fast spliced aligner with low memory requirements. Nat. Methods 12, 357\u2013360 (2015).","journal-title":"Nat. Methods"},{"key":"48140_CR68","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106","volume":"11","author":"S Anders","year":"2010","unstructured":"Anders, S. & Huber, W. Differential expression analysis for sequence count data. Genome Biol. 11, R106 (2010).","journal-title":"Genome Biol."},{"key":"48140_CR69","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.pt.2015.10.003","volume":"32","author":"H Ginsburg","year":"2016","unstructured":"Ginsburg, H. & Abdel-Haleem, A. M. Malaria parasite metabolic pathways (MPMP) upgraded with targeted chemical compounds. Trends Parasitol. 32, 7\u20139 (2016).","journal-title":"Trends Parasitol."},{"key":"48140_CR70","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-7-r134","volume":"8","author":"JL Shock","year":"2007","unstructured":"Shock, J. L., Fischer, K. F. & DeRisi, J. L. Whole-genome analysis of mRNA decay in Plasmodium falciparum reveals a global lengthening of mRNA half-life during the intra-erythrocytic development cycle. Genome Biol. 8, R134 (2007).","journal-title":"Genome Biol."},{"key":"48140_CR71","doi-asserted-by":"publisher","first-page":"2619","DOI":"10.1021\/pr201185r","volume":"11","author":"H Choi","year":"2012","unstructured":"Choi, H., Glatter, T., Gstaiger, M. & Nesvizhskii, A. I. SAINT-MS1: protein-protein interaction scoring using label-free intensity data in affinity purification-mass spectrometry experiments. J. Proteome Res. 11, 2619\u20132624 (2012).","journal-title":"J. Proteome Res."},{"key":"48140_CR72","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-587","volume":"12","author":"MJ L\u00f3pez-Barrag\u00e1n","year":"2011","unstructured":"L\u00f3pez-Barrag\u00e1n, M. J. et al. Directional gene expression and antisense transcripts in sexual and asexual stages of Plasmodium falciparum. BMC Genomics 12, 587 (2011).","journal-title":"BMC Genomics"}],"container-title":["Nature Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-48140-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-48140-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-48140-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,17]],"date-time":"2024-11-17T19:52:06Z","timestamp":1731873126000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-48140-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,3]]},"references-count":72,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["48140"],"URL":"https:\/\/doi.org\/10.1038\/s41467-024-48140-4","relation":{},"ISSN":["2041-1723"],"issn-type":[{"value":"2041-1723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,3]]},"assertion":[{"value":"4 February 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 April 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3747"}}