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Azole antifungals represent first-line therapeutics for most of these infections but resistance is rising, therefore the identification of antifungal targets whose inhibition synergises with the azoles could improve therapeutic outcomes. Here, we generate a library of 111 genetically barcoded null mutants of\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    in genes encoding protein kinases, and show that loss of function of kinase YakA results in hypersensitivity to the azoles and reduced pathogenicity. YakA is an orthologue of\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    Yak1, a TOR signalling pathway kinase involved in modulation of stress responsive transcriptional regulators. We show that YakA has been repurposed\n                    <jats:italic>in A. fumigatus<\/jats:italic>\n                    to regulate blocking of the septal pore upon exposure to stress. Loss of YakA function reduces the ability of\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    to penetrate solid media and to grow in mouse lung tissue. We also show that 1-ethoxycarbonyl-beta-carboline (1-ECBC), a compound previously shown to inhibit\n                    <jats:italic>C. albicans<\/jats:italic>\n                    Yak1, prevents stress-mediated septal spore blocking and synergises with the azoles to inhibit\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    growth.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-48592-8","type":"journal-article","created":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T13:02:54Z","timestamp":1718110974000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Functional analysis of the Aspergillus fumigatus kinome identifies a druggable DYRK kinase that regulates septal plugging"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6722-2757","authenticated-orcid":false,"given":"Norman","family":"van Rhijn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5338-6443","authenticated-orcid":false,"given":"Narjes","family":"Al-Furaiji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle S. 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