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The molecular mechanisms that mediate\n                    <jats:italic>T. gondii<\/jats:italic>\n                    differentiation remain poorly understood. Through a mutagenesis screen, we identified translation initiation factor eIF1.2 as a critical factor for\n                    <jats:italic>T. gondii<\/jats:italic>\n                    differentiation. A F97L mutation in eIF1.2 or the genetic ablation of\n                    <jats:italic>eIF1.2<\/jats:italic>\n                    (\u2206\n                    <jats:italic>eif1.2<\/jats:italic>\n                    ) markedly impeded bradyzoite cyst formation in vitro and in vivo. We demonstrated, at single-molecule level, that the eIF1.2 F97L mutation impacts the scanning process of the ribosome preinitiation complex on a model mRNA. RNA sequencing and ribosome profiling experiments unveiled that \u2206\n                    <jats:italic>eif1.2<\/jats:italic>\n                    parasites are defective in upregulating bradyzoite induction factors BFD1 and BFD2 during stress-induced differentiation. Forced expression of BFD1 or BFD2 significantly restored differentiation in \u2206\n                    <jats:italic>eif1.2<\/jats:italic>\n                    parasites. Together, our findings suggest that eIF1.2 functions by regulating the translation of key differentiation factors necessary to establish chronic toxoplasmosis.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-48685-4","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T08:01:49Z","timestamp":1716451309000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Translation initiation factor eIF1.2 promotes Toxoplasma stage conversion by regulating levels of key differentiation factors"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6778-1962","authenticated-orcid":false,"given":"Fengrong","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4562-287X","authenticated-orcid":false,"given":"Michael J.","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hea Jin","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8058-1743","authenticated-orcid":false,"given":"Pariyamon","family":"Thaprawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geetha","family":"Kannan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"My-Hang","family":"Huynh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3133-8756","authenticated-orcid":false,"given":"Tracey L.","family":"Schultz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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