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However, the structural dynamics by which calmodulin modulates the sarcoplasmic reticulum Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release channel, the ryanodine receptor, at physiologically relevant [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ] is unknown. Using fluorescence lifetime FRET, we resolve different structural states of calmodulin and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -driven shifts in the conformation of calmodulin bound to ryanodine receptor. Skeletal and cardiac ryanodine receptor isoforms show different calmodulin-ryanodine receptor conformations, as well as binding and structural kinetics with 0.2-ms resolution, which reflect different functional roles of calmodulin. These FRET methods provide insight into the physiological calmodulin-ryanodine receptor structural states, revealing additional distinct structural states that complement cryo-EM models that are based on less physiological conditions. This technology will drive future studies on pathological calmodulin-ryanodine receptor interactions and dynamics with other important ryanodine receptor bound modulators.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-48951-5","type":"journal-article","created":{"date-parts":[[2024,6,15]],"date-time":"2024-06-15T04:02:39Z","timestamp":1718424159000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Kinetics and mapping of Ca-driven calmodulin conformations on skeletal and cardiac muscle ryanodine receptors"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3840-2286","authenticated-orcid":false,"given":"Robyn T.","family":"Rebbeck","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengt","family":"Svensson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2788-3283","authenticated-orcid":false,"given":"Montserrat","family":"Sams\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8822-2040","authenticated-orcid":false,"given":"David D.","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2237-9483","authenticated-orcid":false,"given":"Donald M.","family":"Bers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5739-0992","authenticated-orcid":false,"given":"Razvan L.","family":"Cornea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,6,15]]},"reference":[{"key":"48951_CR1","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.cell.2016.08.075","volume":"167","author":"A des Georges","year":"2016","unstructured":"des Georges, A. et al. 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