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Here, we show that Rab30 is induced in the mouse liver by fasting, which is amplified in liver-specific carnitine palmitoyltransferase 2 knockout mice (Cpt2\n                    <jats:sup>L\u2212\/\u2212<\/jats:sup>\n                    ) lacking the ability to oxidize fatty acids, in a Ppar\u03b1-dependent manner. Live-cell super-resolution imaging and in vivo proximity labeling demonstrates that Rab30-marked vesicles are highly dynamic and interact with proteins throughout the secretory pathway. Rab30 whole-body, liver-specific, and Rab30; Cpt2 liver-specific double knockout (DKO) mice are viable with intact Golgi ultrastructure, although Rab30 deficiency in DKO mice suppresses the serum dyslipidemia observed in Cpt2\n                    <jats:sup>L\u2212\/\u2212<\/jats:sup>\n                    mice. Corresponding with decreased serum triglyceride and cholesterol levels, DKO mice exhibit decreased circulating but not hepatic ApoA4 protein, indicative of a trafficking defect. Together, these data suggest a role for Rab30 in the selective sorting of lipoproteins to influence hepatocyte and circulating triglyceride levels, particularly during times of excessive lipid burden.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-48959-x","type":"journal-article","created":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T08:02:04Z","timestamp":1716624124000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Rab30 facilitates lipid homeostasis during fasting"],"prefix":"10.1038","volume":"15","author":[{"given":"Danielle M.","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6856-5491","authenticated-orcid":false,"given":"Brian Y.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2349-8414","authenticated-orcid":false,"given":"Michael J.","family":"Wolfgang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,25]]},"reference":[{"key":"48959_CR1","doi-asserted-by":"publisher","first-page":"2096","DOI":"10.1083\/jcb.201903090","volume":"218","author":"RJ Schulze","year":"2019","unstructured":"Schulze, R. 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