{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T01:02:52Z","timestamp":1793149372671,"version":"4.5.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T00:00:00Z","timestamp":1720656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T00:00:00Z","timestamp":1720656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Commun"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    As bacteriophages continue to gain regulatory approval for personalized human therapy against antibiotic-resistant infections, there is a need for transformative technologies for rapid target identification through multiple, large, decentralized therapeutic phages biobanks. Here, we design a high throughput phage screening platform comprised of a portable library of individual shelf-stable, ready-to-use phages, in all-inclusive solid tablets. Each tablet encapsulates one phage along with luciferin and luciferase enzyme stabilized in a sugar matrix comprised of pullulan and trehalose capable of directly detecting phage-mediated adenosine triphosphate (ATP) release through ATP bioluminescence reaction upon bacterial cell burst. The tablet composition also enhances desiccation tolerance of all components, which should allow easier and cheaper international transportation of phages and as a result, increased accessibility to therapeutic phages. We demonstrate high throughput screening by identifying target phages for select multidrug-resistant clinical isolates of\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    with targets identified within 30-120\u2009min.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-49710-2","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T06:02:48Z","timestamp":1720677768000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["High throughput platform technology for rapid target identification in personalized phage therapy"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8868-1650","authenticated-orcid":false,"given":"Fereshteh","family":"Bayat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7903-0258","authenticated-orcid":false,"given":"Arwa","family":"Hilal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6236-153X","authenticated-orcid":false,"given":"Mathura","family":"Thirugnanasampanthar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Tremblay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7410-3323","authenticated-orcid":false,"given":"Carlos D. M.","family":"Filipe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-5101","authenticated-orcid":false,"given":"Sylvain","family":"Moineau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8757-8002","authenticated-orcid":false,"given":"Tohid F.","family":"Didar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9261-7611","authenticated-orcid":false,"given":"Zeinab","family":"Hosseinidoust","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,11]]},"reference":[{"key":"49710_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/md15060158","volume":"15","author":"JEM Watts","year":"2017","unstructured":"Watts, J. E. M., Schreier, H. J., Lanska, L. & Hale, M. S. The rising tide of antimicrobial resistance in aquaculture: Sources, sinks and solutions. Mar. Drugs 15, 1\u201316 (2017).","journal-title":"Mar. Drugs"},{"key":"49710_CR2","doi-asserted-by":"publisher","first-page":"S27","DOI":"10.12968\/jowc.2016.25.7.S27","volume":"25","author":"R Fish","year":"2016","unstructured":"Fish, R. et al. Bacteriophage treatment of intransigent Diabetic toe ulcers: a case series. J. Wound Care 25, S27\u2013S33 (2016).","journal-title":"J. Wound Care"},{"key":"49710_CR3","doi-asserted-by":"publisher","first-page":"131","DOI":"10.3390\/antibiotics8030131","volume":"8","author":"S Divya Ganeshan","year":"2019","unstructured":"Divya Ganeshan, S. & Hosseinidoust, Z. Phage therapy with a focus on the human microbiota. 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Molecular Cloning: A Laboratory Manual (Cold Spring Harbour Laboratory Press, 2001)."}],"container-title":["Nature Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-49710-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-49710-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-49710-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T06:03:37Z","timestamp":1720677817000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-49710-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,11]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["49710"],"URL":"https:\/\/doi.org\/10.1038\/s41467-024-49710-2","relation":{},"ISSN":["2041-1723"],"issn-type":[{"value":"2041-1723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,11]]},"assertion":[{"value":"2 May 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 June 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 July 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The patent for using pullulan\/trehalose to stabilize biological agents has been licensed to Elarex. Carlos Filipe is a co-founder and currently a scientific advisor to Elarex. A patent application has been filed by McMaster University (inventors F.B, T.F.D, Z.H.) to the Canadian Intellectual Property patent office pertaining to the high throughput aspect(s) of this work (application number PCT\/CA2024\/050748). The remaining authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"5626"}}