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Herein we demonstrate that chemokine CCL25-triggered Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx induces T cell membrane-proximal external Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>ex<\/jats:sub>\n                    ) drop in 6\u2009s from physiological concentration 1.2\u2009mM to 0.3\u2009mM, a critical extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    threshold for inducing \u03b1L\u03b22 activation, triggering rapid \u03b1L\u03b22 activation and T cell arrest before occurrence of \u03b1L\u03b22 inside-out activation. Talin knockdown inhibits the slow inside-out activation of \u03b1L\u03b22 but not [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>ex<\/jats:sub>\n                    drop-triggered \u03b1L\u03b22 quick activation. Blocking Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx significantly suppresses T cell rolling-to-arrest transition and homing to skin lesions in a mouse psoriasis model, thus alleviating skin inflammation. [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>ex<\/jats:sub>\n                    decrease-triggered rapid integrin activation bridges the gap between initial chemokine stimulation and slow integrin inside-out activation, ensuring immediate lymphocyte arrest and subsequent diapedesis on the right location.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-50464-0","type":"journal-article","created":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T14:01:43Z","timestamp":1721484103000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Ca2+ transients on the T cell surface trigger rapid integrin activation in a timescale of seconds"],"prefix":"10.1038","volume":"15","author":[{"given":"Yue","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ShiHui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YouHua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZhaoYuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YunZhe","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ShiYang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiaoYing","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MengWen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XingChao","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YaJuan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MengYa","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3189-8928","authenticated-orcid":false,"given":"GaoXiang","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1898-8099","authenticated-orcid":false,"given":"Yi Arial","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6766-3699","authenticated-orcid":false,"given":"ChangDong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2892-1691","authenticated-orcid":false,"given":"JianFeng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,20]]},"reference":[{"key":"50464_CR1","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.immuni.2019.01.013","volume":"50","author":"N Reglero-Real","year":"2019","unstructured":"Reglero-Real, N., Rolas, L. & Nourshargh, S. 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