{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T07:48:00Z","timestamp":1794037680422,"version":"4.8.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Commun"],"DOI":"10.1038\/s41467-024-50811-1","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T17:02:31Z","timestamp":1722618151000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Capsid structure of bacteriophage \u03a6KZ provides insights into assembly and stabilization of jumbo phages"],"prefix":"10.1038","volume":"15","author":[{"given":"Yashan","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9937-0631","authenticated-orcid":false,"given":"Qianqian","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8043-7303","authenticated-orcid":false,"given":"Mingcheng","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2684-1345","authenticated-orcid":false,"given":"Xinyue","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2214-8471","authenticated-orcid":false,"given":"Aohan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2842-4126","authenticated-orcid":false,"given":"Ji-An","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4748-9299","authenticated-orcid":false,"given":"Zhenguo","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei","family":"Fokine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8741-0202","authenticated-orcid":false,"given":"Lei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1844-7313","authenticated-orcid":false,"given":"Qianglin","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,8,2]]},"reference":[{"key":"50811_CR1","doi-asserted-by":"publisher","first-page":"e00052","DOI":"10.1128\/JB.00052-20","volume":"202","author":"AR Mushegian","year":"2020","unstructured":"Mushegian, A. R. Are there 10(31) virus particles on earth, or more, or fewer? J. Bacteriol. 202, e00052\u201320 (2020).","journal-title":"J. Bacteriol."},{"key":"50811_CR2","first-page":"229","volume":"328","author":"RW Hendrix","year":"2009","unstructured":"Hendrix, R. W. Jumbo bacteriophages. Curr. Top. Microbiol Immunol. 328, 229\u2013240 (2009).","journal-title":"Curr. Top. Microbiol Immunol."},{"key":"50811_CR3","doi-asserted-by":"publisher","first-page":"R750","DOI":"10.1016\/j.cub.2023.05.056","volume":"33","author":"KR Harding","year":"2023","unstructured":"Harding, K. R., Kyte, N. & Fineran, P. C. Jumbo phages. Curr. Biol. 33, R750\u2013R751 (2023).","journal-title":"Curr. Biol."},{"key":"50811_CR4","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1038\/s41586-020-2007-4","volume":"578","author":"B Al-Shayeb","year":"2020","unstructured":"Al-Shayeb, B. et al. Clades of huge phages from across Earth\u2019s ecosystems. Nature 578, 425\u2013431 (2020).","journal-title":"Nature"},{"key":"50811_CR5","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1038\/s41564-019-0612-5","volume":"5","author":"LM Malone","year":"2020","unstructured":"Malone, L. M. et al. A jumbo phage that forms a nucleus-like structure evades CRISPR-Cas DNA targeting but is vulnerable to type III RNA-based immunity. Nat. Microbiol. 5, 48\u201355 (2020).","journal-title":"Nat. Microbiol."},{"key":"50811_CR6","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1038\/s41586-022-05013-4","volume":"608","author":"TG Laughlin","year":"2022","unstructured":"Laughlin, T. G. et al. Architecture and self-assembly of the jumbo bacteriophage nuclear shell. Nature 608, 429\u2013435 (2022).","journal-title":"Nature"},{"key":"50811_CR7","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1126\/science.aal2130","volume":"355","author":"V Chaikeeratisak","year":"2017","unstructured":"Chaikeeratisak, V. et al. Assembly of a nucleus-like structure during viral replication in bacteria. Science 355, 194\u2013197 (2017).","journal-title":"Science"},{"key":"50811_CR8","doi-asserted-by":"publisher","first-page":"7449","DOI":"10.1128\/JVI.00385-15","volume":"89","author":"DP Pires","year":"2015","unstructured":"Pires, D. P., Vilas Boas, D., Sillankorva, S. & Azeredo, J. Phage therapy: a step forward in the treatment of Pseudomonas aeruginosa infections. J. Virol. 89, 7449\u20137456 (2015).","journal-title":"J. Virol."},{"key":"50811_CR9","doi-asserted-by":"publisher","first-page":"149","DOI":"10.3390\/v13020149","volume":"13","author":"V Krylov","year":"2021","unstructured":"Krylov, V. et al. Phage phiKZ-the first of giants. Viruses 13, 149 (2021).","journal-title":"Viruses"},{"key":"50811_CR10","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04051-9","volume":"9","author":"D Zhu","year":"2018","unstructured":"Zhu, D. et al. Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions. Nat. Commun. 9, 1552 (2018).","journal-title":"Nat. Commun."},{"key":"50811_CR11","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/j.jsb.2011.05.004","volume":"175","author":"X Zhang","year":"2011","unstructured":"Zhang, X. & Zhou, Z. H. Limiting factors in atomic resolution cryo electron microscopy: no simple tricks. J. Struct. Biol. 175, 253\u2013263 (2011).","journal-title":"J. Struct. Biol."},{"key":"50811_CR12","doi-asserted-by":"publisher","first-page":"4139","DOI":"10.1016\/j.jmb.2020.05.016","volume":"432","author":"B Gonzalez","year":"2020","unstructured":"Gonzalez, B. et al. Phage G structure at 6.1\u00a0\u00c5\u00a0resolution, condensed DNA, and host identity revision to a lysinibacillus. J. Mol. Biol. 432, 4139\u20134153 (2020).","journal-title":"J. Mol. Biol."},{"key":"50811_CR13","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1016\/j.str.2012.12.017","volume":"21","author":"G Effantin","year":"2013","unstructured":"Effantin, G. et al. Cryo-electron microscopy three-dimensional structure of the jumbo phage PhiRSL1 infecting the phytopathogen Ralstonia solanacearum. Structure 21, 298\u2013305 (2013).","journal-title":"Structure"},{"key":"50811_CR14","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1099\/jgv.0.001487","volume":"101","author":"E Neumann","year":"2020","unstructured":"Neumann, E. et al. 3D structure of three jumbo phage heads. J. Gen. Virol. 101, 1219\u20131226 (2020).","journal-title":"J. Gen. Virol."},{"key":"50811_CR15","doi-asserted-by":"publisher","first-page":"e01579","DOI":"10.1128\/mBio.01579-17","volume":"8","author":"J Hua","year":"2017","unstructured":"Hua, J. et al. Capsids and genomes of jumbo-sized bacteriophages reveal the evolutionary reach of the HK97 fold. mBio 8, e01579\u201317 (2017).","journal-title":"mBio"},{"key":"50811_CR16","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34972-5","volume":"13","author":"R Ouyang","year":"2022","unstructured":"Ouyang, R. et al. High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers. Nat. Commun. 13, 7241 (2022).","journal-title":"Nat. Commun."},{"key":"50811_CR17","doi-asserted-by":"publisher","first-page":"403","DOI":"10.3389\/fmicb.2017.00403","volume":"8","author":"Y Yuan","year":"2017","unstructured":"Yuan, Y. & Gao, M. Jumbo bacteriophages: an overview. Front Microbiol. 8, 403 (2017).","journal-title":"Front Microbiol."},{"key":"50811_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1006\/jmbi.2001.5396","volume":"317","author":"VV Mesyanzhinov","year":"2002","unstructured":"Mesyanzhinov, V. V. et al. The genome of bacteriophage phiKZ of Pseudomonas aeruginosa. J. Mol. Biol. 317, 1\u201319 (2002).","journal-title":"J. Mol. Biol."},{"key":"50811_CR19","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/j.imj.2023.05.003","volume":"2","author":"N Sathe","year":"2023","unstructured":"Sathe, N. et al. Pseudomonas aeruginosa: infections and novel approaches to treatment \u201cKnowing the enemy\u201d the threat of Pseudomonas aeruginosa and exploring novel approaches to treatment. Infect. Med (Beijing) 2, 178\u2013194 (2023).","journal-title":"Infect. Med (Beijing)"},{"key":"50811_CR20","doi-asserted-by":"publisher","first-page":"65","DOI":"10.3389\/fmicb.2011.00065","volume":"2","author":"K Poole","year":"2011","unstructured":"Poole, K. Pseudomonas aeruginosa: resistance to the max. Front Microbiol. 2, 65 (2011).","journal-title":"Front Microbiol."},{"key":"50811_CR21","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1128\/CMR.00040-09","volume":"22","author":"PD Lister","year":"2009","unstructured":"Lister, P. D., Wolter, D. J. & Hanson, N. D. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin. Microbiol Rev. 22, 582\u2013610 (2009).","journal-title":"Clin. Microbiol Rev."},{"key":"50811_CR22","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1186\/s12941-020-00389-5","volume":"19","author":"Z Chegini","year":"2020","unstructured":"Chegini, Z. et al. Bacteriophage therapy against Pseudomonas aeruginosa biofilms: a review. Ann. Clin. Microbiol. Antimicrob. 19, 45 (2020).","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"50811_CR23","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2022.105767","volume":"172","author":"P Rai","year":"2022","unstructured":"Rai, P. et al. Characterisation of broad-spectrum phiKZ like jumbo phage and its utilisation in controlling multidrug-resistant Pseudomonas aeruginosa isolates. Micro. Pathog. 172, 105767 (2022).","journal-title":"Micro. Pathog."},{"key":"50811_CR24","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1007\/s12250-014-3546-3","volume":"30","author":"V Krylov","year":"2015","unstructured":"Krylov, V. et al. Selection of phages and conditions for the safe phage therapy against Pseudomonas aeruginosa infections. Virol. Sin. 30, 33\u201344 (2015).","journal-title":"Virol. Sin."},{"key":"50811_CR25","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.jmb.2005.07.018","volume":"352","author":"A Fokine","year":"2005","unstructured":"Fokine, A. et al. A three-dimensional cryo-electron microscopy structure of the bacteriophage phiKZ head. J. Mol. Biol. 352, 117\u2013124 (2005).","journal-title":"J. Mol. Biol."},{"key":"50811_CR26","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.1016\/j.str.2007.07.008","volume":"15","author":"A Fokine","year":"2007","unstructured":"Fokine, A. et al. Cryo-EM study of the Pseudomonas bacteriophage phiKZ. Structure 15, 1099\u20131104 (2007).","journal-title":"Structure"},{"key":"50811_CR27","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1126\/science.1214120","volume":"335","author":"W Wu","year":"2012","unstructured":"Wu, W., Thomas, J. A., Cheng, N., Black, L. W. & Steven, A. C. Bubblegrams reveal the inner body of bacteriophage phiKZ. Science 335, 182 (2012).","journal-title":"Science"},{"key":"50811_CR28","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1111\/j.1365-2958.2012.08025.x","volume":"84","author":"JA Thomas","year":"2012","unstructured":"Thomas, J. A. et al. Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage phiKZ. Mol. Microbiol. 84, 324\u2013339 (2012).","journal-title":"Mol. Microbiol."},{"key":"50811_CR29","doi-asserted-by":"publisher","first-page":"3215","DOI":"10.1002\/pmic.200800727","volume":"9","author":"E Lecoutere","year":"2009","unstructured":"Lecoutere, E. et al. Identification and comparative analysis of the structural proteomes of phiKZ and EL, two giant Pseudomonas aeruginosa bacteriophages. Proteomics 9, 3215\u20133219 (2009).","journal-title":"Proteomics"},{"key":"50811_CR30","doi-asserted-by":"publisher","first-page":"2129","DOI":"10.1126\/science.289.5487.2129","volume":"289","author":"WR Wikoff","year":"2000","unstructured":"Wikoff, W. R. et al. Topologically linked protein rings in the bacteriophage HK97 capsid. Science 289, 2129\u20132133 (2000).","journal-title":"Science"},{"key":"50811_CR31","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1016\/j.jmb.2003.09.035","volume":"334","author":"C Helgstrand","year":"2003","unstructured":"Helgstrand, C. et al. The refined structure of a protein catenane: the HK97 bacteriophage capsid at 3.44 \u00c5\u00a0resolution. J. Mol. Biol. 334, 885\u2013899 (2003).","journal-title":"J. Mol. Biol."},{"key":"50811_CR32","doi-asserted-by":"publisher","first-page":"E8184","DOI":"10.1073\/pnas.1708483114","volume":"114","author":"Z Chen","year":"2017","unstructured":"Chen, Z. et al. Cryo-EM structure of the bacteriophage T4 isometric head at 3.3-\u00c5 resolution and its relevance to the assembly of icosahedral viruses. Proc. Natl. Acad. Sci. USA 114, E8184\u2013E8193 (2017).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"50811_CR33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2203272119","volume":"119","author":"Q Fang","year":"2022","unstructured":"Fang, Q. et al. Structures of a large prolate virus capsid in unexpanded and expanded states generate insights into the icosahedral virus assembly. Proc. Natl. Acad. Sci. USA 119, e2203272119 (2022).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"50811_CR34","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","volume":"596","author":"J Jumper","year":"2021","unstructured":"Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583\u2013589 (2021).","journal-title":"Nature"},{"key":"50811_CR35","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12341-z","volume":"10","author":"NP Stone","year":"2019","unstructured":"Stone, N. P., Demo, G., Agnello, E. & Kelch, B. A. Principles for enhancing virus capsid capacity and stability from a thermophilic virus capsid structure. Nat. Commun. 10, 4471 (2019).","journal-title":"Nat. Commun."},{"key":"50811_CR36","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.str.2017.12.001","volume":"26","author":"Z Wang","year":"2018","unstructured":"Wang, Z. et al. Structure of the marine siphovirus TW1: evolution of capsid-stabilizing proteins and tail spikes. Structure 26, 238\u2013248 e233 (2018).","journal-title":"Structure"},{"key":"50811_CR37","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08319-6","volume":"10","author":"Q Fang","year":"2019","unstructured":"Fang, Q. et al. Near-atomic structure of a giant virus. Nat. Commun. 10, 388 (2019).","journal-title":"Nat. Commun."},{"key":"50811_CR38","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34218-4","volume":"13","author":"Q Shao","year":"2022","unstructured":"Shao, Q. et al. Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1. Nat. Commun. 13, 6476 (2022).","journal-title":"Nat. Commun."},{"key":"50811_CR39","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/j.str.2021.12.009","volume":"30","author":"C Wang","year":"2022","unstructured":"Wang, C., Zeng, J. & Wang, J. Structural basis of bacteriophage lambda capsid maturation. Structure 30, 637\u2013645 e633 (2022).","journal-title":"Structure"},{"key":"50811_CR40","doi-asserted-by":"publisher","first-page":"E4606","DOI":"10.1073\/pnas.1407020111","volume":"111","author":"F Guo","year":"2014","unstructured":"Guo, F. et al. Capsid expansion mechanism of bacteriophage T7 revealed by multistate atomic models derived from cryo-EM reconstructions. Proc. Natl. Acad. Sci. USA 111, E4606\u2013E4614 (2014).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"50811_CR41","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12790-6","volume":"10","author":"A Ignatiou","year":"2019","unstructured":"Ignatiou, A. et al. Structural transitions during the scaffolding-driven assembly of a viral capsid. Nat. Commun. 10, 4840 (2019).","journal-title":"Nat. Commun."},{"key":"50811_CR42","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40724-w","volume":"14","author":"A Fossati","year":"2023","unstructured":"Fossati, A. et al. Next-generation proteomics for quantitative Jumbophage-bacteria interaction mapping. Nat. Commun. 14, 5156 (2023).","journal-title":"Nat. Commun."},{"key":"50811_CR43","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1139\/m84-116","volume":"30","author":"VN Krylov","year":"1984","unstructured":"Krylov, V. N. et al. Pseudomonas bacteriophage phi KZ contains an inner body in its capsid. Can. J. Microbiol. 30, 758\u2013762 (1984).","journal-title":"Can. J. Microbiol."},{"key":"50811_CR44","doi-asserted-by":"publisher","first-page":"e697","DOI":"10.1002\/cpz1.697","volume":"3","author":"R Zaru","year":"2023","unstructured":"Zaru, R., Orchard, S. & UniProt, C. UniProt tools: BLAST, align, peptide search, and ID mapping. Curr. Protoc. 3, e697 (2023).","journal-title":"Curr. Protoc."},{"key":"50811_CR45","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1038\/nmeth.4193","volume":"14","author":"SQ Zheng","year":"2017","unstructured":"Zheng, S. Q. et al. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nat. Methods 14, 331\u2013332 (2017).","journal-title":"Nat. Methods"},{"key":"50811_CR46","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","volume":"192","author":"A Rohou","year":"2015","unstructured":"Rohou, A. & Grigorieff, N. CTFFIND4: fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192, 216\u2013221 (2015).","journal-title":"J. Struct. Biol."},{"key":"50811_CR47","doi-asserted-by":"publisher","first-page":"4169","DOI":"10.1042\/BCJ20210708","volume":"478","author":"D Kimanius","year":"2021","unstructured":"Kimanius, D., Dong, L., Sharov, G., Nakane, T. & Scheres, S. H. W. New tools for automated cryo-EM single-particle analysis in RELION-4.0. Biochem. J. 478, 4169\u20134185 (2021).","journal-title":"Biochem. J."},{"key":"50811_CR48","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"P Emsley","year":"2010","unstructured":"Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D Biol. Crystallogr. 66, 486\u2013501 (2010).","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"50811_CR49","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"EF Pettersen","year":"2004","unstructured":"Pettersen, E. F. et al. UCSF Chimera-a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605\u20131612 (2004).","journal-title":"J. Comput. Chem."},{"key":"50811_CR50","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1107\/S2052252521011088","volume":"9","author":"G Chojnowski","year":"2022","unstructured":"Chojnowski, G. et al. findMySequence: a neural-network-based approach for identification of unknown proteins in X-ray crystallography and cryo-EM. IUCrJ 9, 86\u201397 (2022).","journal-title":"IUCrJ"},{"key":"50811_CR51","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1107\/S2059798322005009","volume":"78","author":"G Chojnowski","year":"2022","unstructured":"Chojnowski, G. Sequence-assignment validation in cryo-EM models with checkMySequence. Acta Crystallogr. D Struct. Biol. 78, 806\u2013816 (2022).","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"50811_CR52","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"PD Adams","year":"2010","unstructured":"Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, 213\u2013221 (2010).","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"50811_CR53","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.jmb.2009.07.008","volume":"392","author":"F DiMaio","year":"2009","unstructured":"DiMaio, F., Tyka, M. D., Baker, M. L., Chiu, W. & Baker, D. Refinement of protein structures into low-resolution density maps using rosetta. J. Mol. Biol. 392, 181\u2013190 (2009).","journal-title":"J. Mol. Biol."},{"key":"50811_CR54","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1002\/pro.3943","volume":"30","author":"EF Pettersen","year":"2021","unstructured":"Pettersen, E. F. et al. UCSF ChimeraX: structure visualization for researchers, educators, and developers. Protein Sci. 30, 70\u201382 (2021).","journal-title":"Protein Sci."}],"container-title":["Nature Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-50811-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-50811-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-50811-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T18:03:28Z","timestamp":1722621808000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41467-024-50811-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,2]]},"references-count":54,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["50811"],"URL":"https:\/\/doi.org\/10.1038\/s41467-024-50811-1","relation":{},"ISSN":["2041-1723"],"issn-type":[{"value":"2041-1723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,2]]},"assertion":[{"value":"12 January 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 August 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"6551"}}