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We explore this assembly by attaching fluorescent moieties to the parathyroid hormone (PTH) type 1\u00a0receptor (PTH<jats:sub>1<\/jats:sub>R), using PTH as a prototypical peptide hormone, along with \u03b2arr and clathrin, and recording dual-color single-molecule imaging at the plasma membrane of live cells. Here we show that PTH<jats:sub>1<\/jats:sub>R exhibits a near-Brownian diffusion, whereas unbound hormone displays limited mobility and slow lateral diffusion at the cell surface. The formation of the PTH\u2013PTH<jats:sub>1<\/jats:sub>R\u2013\u03b2arr complex occurs in three sequential steps: (1) receptor and ligand collisions, (2) phosphoinositide (PIP<jats:sub>3<\/jats:sub>)-dependent recruitment and conformational change of \u03b2arr molecules at the plasma membrane, and (3) collision of most \u03b2arr molecules with the ligand-bound receptor within clathrin clusters. Our results elucidate the non-random pathway by which PTH\u2013PTH<jats:sub>1<\/jats:sub>R\u2013\u03b2arr complex is formed and unveil the critical role of PIP<jats:sub>3<\/jats:sub> in regulating GPCR signaling.<\/jats:p>","DOI":"10.1038\/s41467-024-54772-3","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T07:47:15Z","timestamp":1733212035000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Fast-diffusing receptor collisions with slow-diffusing peptide ligand assemble the ternary parathyroid hormone\u2013GPCR\u2013arrestin complex"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6880-5874","authenticated-orcid":false,"given":"Jonathan","family":"Pacheco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina A.","family":"Pe\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofya","family":"Savransky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Gidon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6660-3272","authenticated-orcid":false,"given":"Gerald R. 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