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Upon mating, endocrine signals orchestrate a 30% net increase of absorptive epithelium. Mating increases production of the steroid hormone Ecdysone released by the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    ovaries that stimulates intestinal stem cell (ISC) divisions. Here, we uncover the transcription factor\n                    <jats:italic>crooked legs (crol)<\/jats:italic>\n                    as an intraepithelial coordinator of Ecdysone-induced ISC mitosis. For the precise investigation of non-autonomous factors on ISC behaviour, we establish Rapport, a spatiotemporally-controlled dual expression and tracing system for the analysis of paracrine genetic manipulation while tracing ISC behaviour. Rapport tracing reveals that Ecdysone-induced Crol controls mitogenic Wnt\/Wg-ligand expression from epithelial enterocytes activating ISC mitosis. Paracrine Wg stimulation is counterbalanced by Crol-repression of\n                    <jats:italic>string\/CDC25<\/jats:italic>\n                    and\n                    <jats:italic>CyclinB<\/jats:italic>\n                    autonomously in ISC. Rapport-based ISC tumours confirm paracrine stimulation through the Ecdysone-Crol-Wg axis on mitotic behaviour, whereas the autonomous anti-proliferative role of Crol in ISC is conserved in models of colorectal cancer. Finally, mathematical modelling corroborates increasing enterocyte numbers and Wnt\/Wg-degradation to set a stable post-mating intestinal size. Together, our findings provide insights into the complex endocrine growth control mechanisms during mating-induced adaptations and might help untangling pleiotropic hormonal effects observed in gastrointestinal tumorigenesis.\n                  <\/jats:p>","DOI":"10.1038\/s41467-024-55664-2","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T18:07:46Z","timestamp":1736186866000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Steroid hormone-induced wingless ligands tune female intestinal size in Drosophila"],"prefix":"10.1038","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7767-139X","authenticated-orcid":false,"given":"Lisa","family":"Zipper","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9806-5643","authenticated-orcid":false,"given":"Bernat","family":"Corominas-Murtra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6610-6148","authenticated-orcid":false,"given":"Tobias","family":"Reiff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"55664_CR1","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1023\/A:1026332304435","volume":"2","author":"KA Hammond","year":"1997","unstructured":"Hammond, K. 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