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Laboratory studies assessing DENV inhibition by\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    typically have not considered natural frequent mosquito blood feeding behavior. Here, we determine the impact of successive feeding on DENV-2 transmission by\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    in the presence or absence of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    (\n                    <jats:italic>w<\/jats:italic>\n                    AlbB and\n                    <jats:italic>w<\/jats:italic>\n                    MelM strains). We show that successive feeding shortens the extrinsic incubation period (EIP) in wildtype (WT; without\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    ) and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB mosquitoes through enhanced dissemination. Feeding empirical data into models showed that successive feeding increases the probability of WT and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB mosquitoes surviving beyond the EIP. Importantly, the more epidemiologically relevant comparison of the odds of\n                    <jats:italic>w<\/jats:italic>\n                    AlbB mosquitoes surviving beyond the EIP relative to WT, reveals a larger impact of successive feeding on WT than\n                    <jats:italic>w<\/jats:italic>\n                    AlbB. This indicates a strong inhibitory effect of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    even in the context of natural frequent mosquito blood feeding behavior.\n                  <\/jats:p>","DOI":"10.1038\/s41467-025-62352-2","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T19:53:12Z","timestamp":1753818792000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Implications of successive blood feeding on Wolbachia-mediated dengue virus inhibition in Aedes aegypti mosquitoes"],"prefix":"10.1038","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4285-9177","authenticated-orcid":false,"given":"Rebecca M.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8976-9988","authenticated-orcid":false,"given":"Mallery I.","family":"Breban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Braiya L.","family":"Nolan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afeez","family":"Sodeinde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7134-0956","authenticated-orcid":false,"given":"Isabel M.","family":"Ott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7645-7523","authenticated-orcid":false,"given":"Perran A.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0591-0356","authenticated-orcid":false,"given":"Xinyue","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2031-1933","authenticated-orcid":false,"given":"Nathan D.","family":"Grubaugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7518-4014","authenticated-orcid":false,"given":"T. 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