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Induced pluripotent stem cell (iPSC)\u2013based disease models offer improved physiological relevance; however, the high costs of media and differentiation procedures limit large-scale testing. Here, we develop a high-throughput conditioned-media-based screening system\u2014the High-throughput screening technology for Aggregation Inhibitors of Diseased cell-derived Aggregative Proteins (HaiDap) system\u2014to identify inhibitors of aggregation induced by iPSC-secreted amyloid \u03b2 (A\u03b2). Using conditioned media derived from differentiated iPSCs of a male Alzheimer\u2019s disease patient, we screen extracts from 22 edible plants. Whereas PBS-based assays showed 40.9% (9\/22) apparent selectivity, the HaiDap system demonstrates higher specificity (13.6%; 3\/22). All three identified extracts (\n                    <jats:italic>O. aristatus<\/jats:italic>\n                    ,\n                    <jats:italic>S. aromaticum<\/jats:italic>\n                    , and\n                    <jats:italic>G. yesoense<\/jats:italic>\n                    ) significantly delay A\u03b2 aggregation on neuronal surfaces in an iPSC-based assay. These findings suggest that the HaiDap system enables efficient, accurate, and low-cost screening of amyloid aggregation inhibitors.\n                  <\/jats:p>","DOI":"10.1038\/s41467-026-71078-8","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T08:14:59Z","timestamp":1774340099000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A high-throughput conditioned-media-based screening system identifies inhibitors of aggregation induced by iPSC-secreted amyloid \u03b2"],"prefix":"10.1038","volume":"17","author":[{"given":"Masahiro","family":"Kuragano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Nishishita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8717-5885","authenticated-orcid":false,"given":"Koki","family":"Araya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taro Q. 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This research was partially funded by Kaneka Corporation. A patent related to the HaiDap system described in this manuscript has been filed. Patent applicant: Kaneka Corporation and Muroran Institute of Technology. Inventor(s): N.N., A.K., K.T., K.U., M.K. Application number: US.202218285090.A and JP2023511699A, Status: published. The remaining authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"4373"}}