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The ability of\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    cells to parasitise erythrocytes depends on distinctive intracellular compartments associated with invasion, as well as the development of unique cellular niches within the infected host cell. However, our understanding of the biology of the malaria parasite is limited by the fact that a large proportion of the parasite\u2019s proteome has no known cellular location or function. To address this problem, we have generated comprehensive high-resolution maps of protein subcellular localisation for the invasive stage of the erythrocytic life cycle of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    , the major cause of malaria mortality. Using the spatial proteomics technique hyperplexed Localisation of Organelle Proteins by Isotopic Tagging (hyperLOPIT) we generated data for 3000\u2009\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    proteins expressed in late schizont stages. Our hyperLOPIT data resolve 24 distinct cellular niches, and using supervised machine-learning we can classify 1646 proteins into one of these compartments including exported sites within the host cell. Through comparative genomic analyses our data resolve the spatial patterns of cell evolution that have shaped the development of\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species and ongoing adaptive pressures and responses that challenge our efforts to manage these major disease-causing organisms.\n                  <\/jats:p>","DOI":"10.1038\/s41467-026-73664-2","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T04:15:44Z","timestamp":1780114544000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The spatial proteome of the Plasmodium falciparum schizont illuminates the composition and evolutionary trajectories of its organelles"],"prefix":"10.1038","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3833-8854","authenticated-orcid":false,"given":"Scott A.","family":"Chisholm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4186-9151","authenticated-orcid":false,"given":"Victor","family":"Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4845-0568","authenticated-orcid":false,"given":"Alison","family":"Kemp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8918-7171","authenticated-orcid":false,"given":"Lisa M.","family":"Breckels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9979-3172","authenticated-orcid":false,"given":"Ludek","family":"Koreny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1959-194X","authenticated-orcid":false,"given":"Nicolas","family":"Dos Santos Pacheco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3580-0345","authenticated-orcid":false,"given":"Konstantin","family":"Barylyuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Kuroshchenkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn S.","family":"Lilley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9835-1014","authenticated-orcid":false,"given":"Julian C.","family":"Rayner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6961-9344","authenticated-orcid":false,"given":"Ross F.","family":"Waller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,30]]},"reference":[{"key":"73664_CR1","unstructured":"World Health Organization. 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