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Plants"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In animals and plants, organ shape is primarily determined during primordium development by carefully coordinated growth and cell division\n                    <jats:sup>1\u20133<\/jats:sup>\n                    . Rare examples of post-primordial change in morphology (reshaping) exist that offer tractable systems for the study of mechanisms required for organ shape determination and diversification. One such example is morphogenesis in\n                    <jats:italic>Capsella<\/jats:italic>\n                    fruits whose heart-shaped appearance emerges by reshaping of the ovate spheroid gynoecium upon fertilization\n                    <jats:sup>4<\/jats:sup>\n                    . Here we use whole-organ live-cell imaging and single-cell RNA sequencing (scRNA-seq) analysis to show that\n                    <jats:italic>Capsella<\/jats:italic>\n                    fruit shape determination is based on dynamic changes in cell growth and cell division coupled with local maintenance of meristematic identity. At the molecular level, we reveal an auxin-induced mechanism that is required for morphological alteration and ultimately determined by a single\n                    <jats:italic>cis<\/jats:italic>\n                    -regulatory element. This element resides in the promoter of the\n                    <jats:italic>Capsella rubella SHOOTMERISTEMLESS<\/jats:italic>\n                    <jats:sup>5<\/jats:sup>\n                    (\n                    <jats:italic>Cr<\/jats:italic>\n                    <jats:italic>STM<\/jats:italic>\n                    ) gene. The CrSTM meristem identity factor positively regulates its own expression through binding to this element, thereby providing a feed-forward loop at the position and time of protrusion emergence to form the heart. Independent evolution of the STM-binding element in\n                    <jats:italic>STM<\/jats:italic>\n                    promoters across Brassicaceae species correlates with those undergoing a gynoecium-to-fruit shape change. Accordingly, genetic and phenotypic studies show that the STM-binding element is required to facilitate the shape transition and suggest a conserved molecular mechanism for organ morphogenesis.\n                  <\/jats:p>","DOI":"10.1038\/s41477-024-01854-1","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T05:04:21Z","timestamp":1733979861000},"page":"23-35","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Evolution of a SHOOTMERISTEMLESS transcription factor binding site promotes fruit shape determination"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1763-7994","authenticated-orcid":false,"given":"Zhi-Cheng","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3405-2901","authenticated-orcid":false,"given":"Mateusz","family":"Majda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0655-1506","authenticated-orcid":false,"given":"Hao-Ran","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3225-8255","authenticated-orcid":false,"given":"Yao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5840-4458","authenticated-orcid":false,"given":"Yi-Ning","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7062-3519","authenticated-orcid":false,"given":"Quan","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3411-7341","authenticated-orcid":false,"given":"Tong-Bing","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3110-1405","authenticated-orcid":false,"given":"Tian-Feng","family":"L\u00fc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4440-8306","authenticated-orcid":false,"given":"Feng","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1510-5505","authenticated-orcid":false,"given":"Gui-Xia","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9220-0787","authenticated-orcid":false,"given":"Richard S.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8497-7657","authenticated-orcid":false,"given":"Lars","family":"\u00d8stergaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2117-538X","authenticated-orcid":false,"given":"Yang","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,12]]},"reference":[{"key":"1854_CR1","doi-asserted-by":"publisher","first-page":"2549","DOI":"10.1242\/dev.101444","volume":"141","author":"MJ Harding","year":"2014","unstructured":"Harding, M. 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