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Plants"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The intersection of actin filaments with the plasma membrane (PM) in plant cells involves lipid\u2013protein interactions by the\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    class VIII myosin ATM2 that are currently not understood. Using pollen tube cells as a model, we describe how ATM2 enables actin\u2013PM contacts by modulating membrane lipid nano-organization. ATM2 bound anionic PM lipids, including phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    ), with no apparent preference for any particular lipid species. Overexpression of ATM2 unexpectedly stabilized actin dynamics and altered pollen tube morphology. Actin dynamics remained unaltered upon overexpression of a lipid-binding-defective ATM2 variant. ATM2 effects on actin dynamics required PtdIns(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    , as artificial PtdIns(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    depletion in pollen tubes abolished the effects of full-length ATM2 on actin dynamics despite its retained PM association. ATM2 colocalized and interacted with the PI4P 5-kinase PIP5K2, which resides at actin\u2013PM contacts and forms PtdIns(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    nanodomains facilitating rho-of-plants-dependent actin stabilization. Upon ATM2 overexpression, a fluorescent PtdIns(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    biosensor decorated an extended PM region in vivo, indicating a promoting effect of ATM2 on PtdIns(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    abundance. The catalytic activity of purified recombinant PIP5K2 protein was enhanced upon coincubation with a purified carboxy-terminal ATM2 fragment in vitro, suggesting that ATM2 contributes to the intrinsic regulation of PtdIns(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    formation. Actin dynamics are thus stabilized at the actin\u2013PM interface by ATM2 locally promoting the formation of PtdIns(4,5)P\n                    <jats:sub>2<\/jats:sub>\n                    nanodomains, thereby self-reinforcing ATM2 recruitment while also facilitating rho of plants activation to stabilize membrane-proximal actin filaments.\n                  <\/jats:p>","DOI":"10.1038\/s41477-026-02427-0","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:47:54Z","timestamp":1792190874000},"page":"2089-2107","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The class VIII myosin ATM2 stabilizes actin\u2013plasma membrane contacts in tobacco pollen tubes by locally promoting PtdIns(4,5)P2 formation"],"prefix":"10.1038","volume":"12","author":[{"given":"Vera C.","family":"Wagner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0934-3854","authenticated-orcid":false,"given":"Marta","family":"Fratini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Uhlenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Schutkowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gottfried","family":"Hamm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Kastner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Bacia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate\u0159ina","family":"Schwarzerov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2324-1849","authenticated-orcid":false,"given":"Ingo","family":"Heilmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"2427_CR1","doi-asserted-by":"publisher","first-page":"kiae558","DOI":"10.1093\/plphys\/kiae558","volume":"197","author":"C Fritz","year":"2024","unstructured":"Fritz, C. et al. 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