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We utilized a well-characterized human three-dimensional cervical epithelial cell model in conjunction with untargeted metabolomics and immunoproteomics analyses to determine the immunometabolic contribution of three members of the <jats:italic>Veillonellaceae<\/jats:italic> family: <jats:italic>Veillonella atypica<\/jats:italic>, <jats:italic>Veillonella montpellierensis<\/jats:italic> and <jats:italic>Megasphaera micronuciformis<\/jats:italic> at this site. We found that <jats:italic>Veillonella<\/jats:italic> spp. infections induced significant elevation of polyamines. <jats:italic>M. micronuciformis<\/jats:italic> infections significantly increased soluble inflammatory mediators, induced moderate levels of cell cytotoxicity, and accumulation of cell membrane lipids relative to <jats:italic>Veillonella<\/jats:italic> spp. Notably, both <jats:italic>V. atypica<\/jats:italic> and <jats:italic>V. montpellierensis<\/jats:italic> infections resulted in consumption of lactate, a key metabolite linked to gynecologic and reproductive health. Collectively our approach and data provide unique insights into the specific contributions of <jats:italic>Veillonellaceae<\/jats:italic> members to the pathogenesis of BV and women\u2019s health.<\/jats:p>","DOI":"10.1038\/s41522-021-00229-0","type":"journal-article","created":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T10:05:09Z","timestamp":1625565909000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model"],"prefix":"10.1038","volume":"7","author":[{"given":"Mary E.","family":"Salliss","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason D.","family":"Maarsingh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camryn","family":"Garza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5190-2173","authenticated-orcid":false,"given":"Pawe\u0142","family":"\u0141aniewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa M.","family":"Herbst-Kralovetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,6]]},"reference":[{"key":"229_CR1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-020-00875-0","volume":"8","author":"G Berg","year":"2020","unstructured":"Berg, G. et al. 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