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Infections are difficult to treat with conventional antibiotics due to the formation of biofilm on the implant surface. We have developed a noninvasive method to eliminate biofilm on metal implants using heat generated by intermittent alternating magnetic fields (iAMF). Here, we demonstrate that heat and antibiotics are synergistic in biofilm elimination. For<jats:italic>Pseudomonas aeruginosa<\/jats:italic>biofilm, bacterial burden was reduced &gt;3 log with iAMF and ciprofloxacin after 24\u2009h compared with either treatment alone (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). This effect was not limited by pathogen or antibiotic as similar biofilm reductions were seen with iAMF and either linezolid or ceftriaxone in<jats:italic>Staphylococcus aureus<\/jats:italic>. iAMF and antibiotic efficacy was seen across various iAMF settings, including different iAMF target temperatures, dose durations, and dosing intervals. Initial mechanistic studies revealed membrane disruption as one factor important for AMF enhanced antibacterial activity in the biofilm setting. This study demonstrates the potential of utilizing a noninvasive approach to reduce biofilm off of metallic implants.<\/jats:p>","DOI":"10.1038\/s41522-021-00239-y","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T10:03:40Z","timestamp":1628762620000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion"],"prefix":"10.1038","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4821-293X","authenticated-orcid":false,"given":"Qi","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4927-6492","authenticated-orcid":false,"given":"Jonathan","family":"Vachon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bibin","family":"Prasad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine A.","family":"Pybus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman","family":"Lapin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3605-6076","authenticated-orcid":false,"given":"Rajiv","family":"Chopra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5101-3106","authenticated-orcid":false,"given":"David E.","family":"Greenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,12]]},"reference":[{"key":"239_CR1","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1038\/s41579-018-0019-y","volume":"16","author":"CR Arciola","year":"2018","unstructured":"Arciola, C. 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Greenberg and Dr. Rajiv Chopra are inventors of the technology related to AMF, holds patents related to this technology, and are the founders of Solenic Medical. Dr. David E. Greenberg serves as Chief Medical Officer and Dr. Rajiv Chopra serves as Chief Technology Officer at Solenic Medical.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"68"}}