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A transposon-based mutation library of a closely related species,\n                    <jats:italic>Mycobacterium marinum<\/jats:italic>\n                    , was used to identify clones in which the relative amount of extracellular DNA (eDNA), an important component of the extracellular matrix of biofilms, is altered. The disruption of a putative glycosyl transferase gene QDR78 11175,\n                    <jats:italic>epsH<\/jats:italic>\n                    , caused a substantial increase of the eDNA content of biofilms, and increased the growth rate and the biomass\/cell in biofilm-forming conditions compared to wild-type. The increased abundance of biomass was mainly due to the elevated levels of eDNA and proteins in the extracellular matrix. The growth of the \u0394\n                    <jats:italic>epsH<\/jats:italic>\n                    strain in the zebrafish was normal, but the mutant developed greater antibiotic tolerance in the adult zebrafish model. 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