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We sought to identify specific chemical inhibitors of\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    biofilm formation that reduced the activity of MrkH. A compound N-(3-cyano-5,6,7,8-tetrahydro-4H-cyclohepta[b]thien-2-yl)-2-methoxybenzamide, JT71, reduced\n                    <jats:italic>K. pneumoniae mrkA<\/jats:italic>\n                    promoter activity and biofilm formation by 50% without affecting cell viability. Western blot analysis, hemagglutination assays, electron microscopy and qPCR showed that JT71 reduced type 3 fimbriae production, and transcription of\n                    <jats:italic>mrkA<\/jats:italic>\n                    and\n                    <jats:italic>mrkH<\/jats:italic>\n                    . JT71 demonstrated activity against other clinical and multi-drug resistant\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates, and a type 3 fimbriate-positive\n                    <jats:italic>Citrobacter koseri<\/jats:italic>\n                    strain. In silico molecule docking was used to illustrate that JT71 could bind directly to the MrkH protein and block its activity. JT71 possesses promising drug-likeness properties and is non-toxic to mammalian cells. Chemical inhibition of transcriptional regulators that control fimbriae expression can inhibit bacterial biofilm formation.\n                  <\/jats:p>","DOI":"10.1038\/s41522-025-00834-3","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T12:03:30Z","timestamp":1763640210000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Chemical inhibition of MrkH-dependent activation of type 3 fimbriae synthesis and biofilm formation by Klebsiella pneumoniae"],"prefix":"10.1038","volume":"11","author":[{"given":"Jonathan J.","family":"Wilksch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason W. 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