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Traditional\n                    <jats:italic>Pf<\/jats:italic>\n                    Ago based detection methods relies on the input of exogenous guide DNA (gDNA), which restricted its flexibility and universality in application. Here, we designed primers with deoxy inosine base which can be recognized and cleaved by endonuclease V, following turn into gDNA to activate\n                    <jats:italic>Pf<\/jats:italic>\n                    Ago for target gene detection. Therefore, an Endo\n                    <jats:underline>V<\/jats:underline>\n                    activated\n                    <jats:italic>\n                      <jats:underline>P<\/jats:underline>\n                    <\/jats:italic>\n                    <jats:italic>f<\/jats:italic>\n                    Ago based\n                    <jats:underline>n<\/jats:underline>\n                    ucleic acid detection (VPN) method was developed. The detection limit of this method was 0.04\u2009ng\/\u03bcL DNA. Moreover, the method was successfully applied to the detection of food contaminated bacteria. This approach provided a universal and power tool for the detection of nucleic acid-containing organisms such as pathogens, viruses, and tumor cells.\n                  <\/jats:p>","DOI":"10.1038\/s41538-025-00675-6","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T03:34:36Z","timestamp":1767152076000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Endonuclease V activated Pyrococcus furiosus Argonaute for the detection of food contaminated bacteria"],"prefix":"10.1038","volume":"10","author":[{"given":"Yiheng","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongning","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"675_CR1","doi-asserted-by":"publisher","first-page":"1685","DOI":"10.1016\/j.bios.2008.08.053","volume":"24","author":"PP Banada","year":"2009","unstructured":"Banada, P. 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