{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T16:02:52Z","timestamp":1784649772251,"version":"3.55.0"},"reference-count":0,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000076","name":"Directorate for Biological Sciences","doi-asserted-by":"publisher","award":["2021132"],"award-info":[{"award-number":["2021132"]}],"id":[{"id":"10.13039\/100000076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AR079545"],"award-info":[{"award-number":["R01 AR079545"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["npj Sci Food"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cultivated meat (CM) scaffolds must be edible and produced with high throughput. This study introduces a novel approach for structured CM production using alginate-based scaffolds prepared using a liquid-liquid extrusion method called Pressurized Apparatus for Striated Tissue Assembly (PASTA). We hypothesized that PASTA scaffolds could enable the creation of tissue constructs exceeding 500\u2009\u00b5m thickness with food-scale throughput. Leveraging sub-boiling hydrothermal degradation, we modulated the biophysical properties of alginate, resulting in controlled reductions in viscosity, zeta potential, and hydrogel mechanical properties. PASTA extrusion consistently produced hydrogel fibers 10\u201342% smaller than the extrusion outlet diameter. Murine C2C12 myoblasts cultured on PASTA scaffolds modified with adhesive peptides adhered, proliferated, and differentiated along the noodle axis. This was confirmed by a 3.5-fold increase in DNA quantity over 4 days, substantial cell viability after 5 days in culture, late-stage myogenic gene expression (\n                    <jats:italic>Myog, Myh2<\/jats:italic>\n                    ), and positive immunohistochemistry for myosin heavy chain. PASTA enables rapid, parallelizable production of aligned fiber scaffolds with physiologically relevant dimensions using inexpensive, food-grade alginate. These findings demonstrate a proof-of-concept scalable and modular platform to produce cell-laden tissues for manufacturing structured CM.\n                  <\/jats:p>","DOI":"10.1038\/s41538-026-00994-2","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:05:58Z","timestamp":1784646358000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Extrusion of Myotube-Scale Alginate Fibers for Structured Cultivated Meat"],"prefix":"10.1038","author":[{"given":"Alex S.","family":"Kermani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea C.","family":"Filler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edrin C.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Block","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Kent","family":"Leach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,21]]},"container-title":["npj Science of Food"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41538-026-00994-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:05:58Z","timestamp":1784646358000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41538-026-00994-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,21]]},"references-count":0,"alternative-id":["994"],"URL":"https:\/\/doi.org\/10.1038\/s41538-026-00994-2","relation":{},"ISSN":["2396-8370"],"issn-type":[{"value":"2396-8370","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,21]]},"assertion":[{"value":"30 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Ethics"}}]}}