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Enteritidis) is one of most common\n                    <jats:italic>Salmonella<\/jats:italic>\n                    serovars associated with human illness in the U.S. and worldwide. Surveillance from the U.S. National Antimicrobial Resistance Monitoring System indicates an increase in both chicken and human isolates of\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis with decreased susceptibility to ciprofloxacin (DSC), a critical antibiotic prescribed for complicated human salmonellosis infections.\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis reduction in chickens is a priority of poultry producers and public health agencies to improve food safety. In the current study, efficacy assessment of a live\n                    <jats:italic>Salmonella<\/jats:italic>\n                    vaccine (BBS 1134) revealed significant reduction of cecal and splenic colonization, and prevention of dissemination to the bone marrow by DSC\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis in broiler chickens. Microbiome analysis indicated the cecal microbiota of vaccinated chickens is distinct compared to mock-vaccinated birds. The IDEXX SE Ab X2 Test did not detect antibodies to\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis in vaccinated chicken serum, thereby permitting differentiation of infected from vaccinated animals (DIVA). Altogether, the\n                    <jats:italic>Salmonella<\/jats:italic>\n                    vaccine is a DIVA vaccine, afforded cross-protection, and significantly reduced intestinal colonization and dissemination to the spleen and bone marrow by DSC\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis in chickens, thereby offering a prospective intervention for animal production to reduce food product contamination and improve food safety.\n                  <\/jats:p>","DOI":"10.1038\/s41541-026-01414-y","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T15:47:32Z","timestamp":1773071252000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Chicken vaccination reduces colonization and dissemination of Salmonella serovar Enteritidis with decreased susceptibility to ciprofloxacin"],"prefix":"10.1038","volume":"11","author":[{"given":"Bradley L.","family":"Bearson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel J.","family":"Whelan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya P. 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