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However, a detailed characterization of lymphoma-infiltrating T cells across B-NHL entities is missing. Here we present an in-depth T cell reference map of nodal B-NHL, based on cellular indexing of transcriptomes and epitopes, T cell receptor sequencing, flow cytometry and multiplexed immunofluorescence applied to 101 lymph nodes from patients with diffuse large B cell, mantle cell, follicular or marginal zone lymphoma, and from healthy controls. This multimodal resource revealed quantitative and spatial aberrations of the T cell microenvironment across and within B-NHL entities. Quantitative differences in PD1\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>TCF7<\/jats:italic>\n                    <jats:sup>\u2212<\/jats:sup>\n                    cytotoxic T cells, T follicular helper cells or IKZF3\n                    <jats:sup>+<\/jats:sup>\n                    regulatory T cells were linked to their clonal expansion. The abundance of PD1\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>TCF7<\/jats:italic>\n                    <jats:sup>\u2212<\/jats:sup>\n                    cytotoxic T cells was associated with poor survival. Our study portrays lymphoma-infiltrating T cells with unprecedented comprehensiveness and provides a unique resource for the investigation of lymphoma biology and prognosis.\n                  <\/jats:p>","DOI":"10.1038\/s41556-024-01358-2","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T06:04:24Z","timestamp":1708409064000},"page":"478-489","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Multimodal and spatially resolved profiling identifies distinct patterns of T cell infiltration in nodal B cell lymphoma entities"],"prefix":"10.1038","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6973-3531","authenticated-orcid":false,"given":"Tobias","family":"Roider","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4000-6904","authenticated-orcid":false,"given":"Marc 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