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Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and INM tension (T\n                    <jats:sub>INM<\/jats:sub>\n                    ) are thought to govern the interactions and activity of cPLA\n                    <jats:sub>2<\/jats:sub>\n                    at the INM. However, as compensatory membrane flow from the contiguous endoplasmic reticulum (ER) may prevent T\n                    <jats:sub>INM<\/jats:sub>\n                    , the conditions permitting nuclear membrane mechanotransduction by cPLA\n                    <jats:sub>2<\/jats:sub>\n                    or other mediators remain unclear. To test whether the ER buffers T\n                    <jats:sub>INM<\/jats:sub>\n                    , we created the genetically encoded, Ca\u00b2\u207a-insensitive T\n                    <jats:sub>INM<\/jats:sub>\n                    biosensor amphipathic lipid-packing domain inside the nucleus (ALPIN). Confocal time-lapse imaging of ALPIN\u2013 or cPLA\n                    <jats:sub>2<\/jats:sub>\n                    \u2013INM interactions, along with ER morphology, nuclear shape\/volume and cell lysis revealed a link between T\n                    <jats:sub>INM<\/jats:sub>\n                    and disrupted ER\u2013nuclear membrane contiguity in osmotically or ferroptotically stressed mammalian cells and at zebrafish wound margins in vivo. By combining ALPIN imaging with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -induced ER disruption, we reveal the causality of this correlation, which suggests that compensatory membrane flow from the ER buffers T\n                    <jats:sub>INM<\/jats:sub>\n                    without preventing it. Besides consolidating the biomechanical basis of cPLA\n                    <jats:sub>2<\/jats:sub>\n                    activation by nuclear deformation, our results identify cell stress- and cell death-induced ER disruption as an additional nuclear membrane mechanotransduction trigger.\n                  <\/jats:p>","DOI":"10.1038\/s41556-025-01820-9","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T11:01:29Z","timestamp":1765278089000},"page":"125-134","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Endoplasmic reticulum disruption stimulates nuclear membrane mechanotransduction"],"prefix":"10.1038","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6524-8993","authenticated-orcid":false,"given":"Zhouyang","family":"Shen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2420-3916","authenticated-orcid":false,"given":"Zaza","family":"Gelashvili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4175-4012","authenticated-orcid":false,"given":"Philipp","family":"Niethammer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,9]]},"reference":[{"key":"1820_CR1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1146\/annurev-cellbio-120319-030049","volume":"37","author":"P Niethammer","year":"2021","unstructured":"Niethammer, P. 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