{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:30:04Z","timestamp":1790803804633,"version":"4.1.0"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T00:00:00Z","timestamp":1517184000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T00:00:00Z","timestamp":1517184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Microbiol"],"DOI":"10.1038\/s41564-017-0099-x","type":"journal-article","created":{"date-parts":[[2018,1,26]],"date-time":"2018-01-26T11:49:43Z","timestamp":1516967383000},"page":"287-294","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Structural basis for neutralization of Japanese encephalitis virus by two potent therapeutic antibodies"],"prefix":"10.1038","volume":"3","author":[{"given":"Xiaodi","family":"Qiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingfeng","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyu","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianbing","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanglin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan-Lu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0632-2807","authenticated-orcid":false,"given":"Chengfeng","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0635-278X","authenticated-orcid":false,"given":"Xiangxi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8893-8444","authenticated-orcid":false,"given":"Zhikai","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9866-2384","authenticated-orcid":false,"given":"Zihe","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,1,29]]},"reference":[{"key":"99_CR1","doi-asserted-by":"publisher","first-page":"766","DOI":"10.2471\/BLT.10.085233","volume":"89","author":"GL Campbell","year":"2011","unstructured":"Campbell, G. L. et al. Estimated global incidence of Japanese encephalitis: a systematic review. B. World Health Organ. 89, 766\u2013774 (2011).","journal-title":"B. World Health Organ."},{"key":"99_CR2","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1089\/088282403322396082","volume":"16","author":"MS Diamond","year":"2003","unstructured":"Diamond, M. S., Shrestha, B., Mehlhop, E., Sitati, E. & Engle, M. Innate and adaptive immune responses determine protection against disseminated infection by West Nile encephalitis virus. Viral Immunol. 16, 259\u2013278 (2003).","journal-title":"Viral Immunol."},{"key":"99_CR3","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1111\/j.1749-6632.2001.tb02704.x","volume":"951","author":"JT Roehrig","year":"2001","unstructured":"Roehrig, J. T., Staudinger, L. A., Hunt, A. R., Mathews, J. H. & Blair, C. D. Antibody prophylaxis and therapy for flavivirus encephalitis infections. Ann. NY Acad. Sci. 951, 286\u2013297 (2001).","journal-title":"Ann. NY Acad. Sci."},{"key":"99_CR4","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00024-6","volume":"8","author":"X Wang","year":"2017","unstructured":"Wang, X. et al. Near-atomic structure of Japanese encephalitis virus reveals critical determinants of virulence and stability. Nat. Commun. 8, 14 (2017).","journal-title":"Nat. Commun."},{"key":"99_CR5","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1002\/jmv.1890290211","volume":"29","author":"MJ Zhang","year":"1989","unstructured":"Zhang, M. J., Wang, M. J., Jiang, S. Z. & Ma, W. Y. Passive protection of mice, goats, and monkeys against Japanese encephalitis with monoclonal antibodies. J. Med. Virol. 29, 133\u2013138 (1989).","journal-title":"J. Med. Virol."},{"key":"99_CR6","doi-asserted-by":"publisher","first-page":"6795","DOI":"10.1073\/pnas.1304300110","volume":"110","author":"X Zhang","year":"2013","unstructured":"Zhang, X. et al. Dengue structure differs at the temperatures of its human and mosquito hosts. Proc. Natl Acad. Sci. USA 110, 6795\u20136799 (2013).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"99_CR7","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1038\/nmeth.2115","volume":"9","author":"SH Scheres","year":"2012","unstructured":"Scheres, S. H. & Chen, S. Prevention of overfitting in cryo-EM structure determination. Nat. Methods 9, 853\u2013854 (2012).","journal-title":"Nat. Methods"},{"key":"99_CR8","doi-asserted-by":"publisher","first-page":"1932","DOI":"10.1021\/acs.jcim.7b00091","volume":"57","author":"L Simhaev","year":"2017","unstructured":"Simhaev, L., McCarty, N. A., Ford, R. C. & Senderowitz, H. Molecular dynamics flexible fitting simulations identify new models of the closed state of the cystic fibrosis transmembrane conductance regulator protein. J. Chen. Inf. Model. 57, 1932\u20131946 (2017).","journal-title":"J. Chen. Inf. Model."},{"key":"99_CR9","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1107\/S0907444912001308","volume":"68","author":"PV Afonine","year":"2012","unstructured":"Afonine, P. V. et al. Towards automated crystallographic structure refinement with phenix. refine. Acta Crystallogr. D 68, 352\u2013367 (2012).","journal-title":"Acta Crystallogr. D"},{"key":"99_CR10","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.coviro.2012.12.001","volume":"3","author":"TC Pierson","year":"2013","unstructured":"Pierson, T. C. & Kielian, M. Flaviviruses: braking the entering. Curr. Opin. Virol. 3, 3\u201312 (2013).","journal-title":"Curr. Opin. Virol."},{"key":"99_CR11","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1016\/S0092-8674(02)00660-8","volume":"108","author":"RJ Kuhn","year":"2002","unstructured":"Kuhn, R. J. et al. Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell 108, 717\u2013725 (2002).","journal-title":"Cell"},{"key":"99_CR12","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1038\/nature02165","volume":"427","author":"Y Modis","year":"2004","unstructured":"Modis, Y., Ogata, S., Clements, D. & Harrison, S. C. Structure of the dengue virus envelope protein after membrane fusion. Nature 427, 313\u2013319 (2004).","journal-title":"Nature"},{"key":"99_CR13","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7341","volume":"6","author":"G Fibriansah","year":"2015","unstructured":"Fibriansah, G. et al. A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins. Nat. Commun. 6, 6341 (2015).","journal-title":"Nat. Commun."},{"key":"99_CR14","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1126\/science.aaa8651","volume":"349","author":"G Fibriansah","year":"2015","unstructured":"Fibriansah, G. et al. Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers. Science 349, 88\u201391 (2015).","journal-title":"Science"},{"key":"99_CR15","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1038\/nature14130","volume":"520","author":"A Rouvinski","year":"2015","unstructured":"Rouvinski, A. et al. Recognition determinants of broadly neutralizing human antibodies against dengue viruses. Nature 520, 109\u2013113 (2015).","journal-title":"Nature"},{"key":"99_CR16","doi-asserted-by":"publisher","first-page":"139ra183","DOI":"10.1126\/scitranslmed.3003888","volume":"4","author":"EP Teoh","year":"2012","unstructured":"Teoh, E. P. et al. The structural basis for serotype-specific neutralization of dengue virus by a human antibody. Sci. Transl. Med. 4, 139ra183 (2012).","journal-title":"Sci. Transl. Med."},{"key":"99_CR17","doi-asserted-by":"crossref","unstructured":"Smith, S. A. et al. The potent and broadly neutralizing human dengue virus-specific monoclonal antibody 1C19 reveals a unique cross-reactive epitope on the bc loop of domain II of the envelope protein. mBio 00873\u201300813 (2013).","DOI":"10.1128\/mBio.00873-13"},{"key":"99_CR18","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1016\/j.chom.2016.04.013","volume":"19","author":"L Dai","year":"2016","unstructured":"Dai, L. et al. Structures of the Zika virus envelope protein and its complex with a flavivirus broadly protective antibody. Cell Host Microbe 19, 696\u2013704 (2016).","journal-title":"Cell Host Microbe"},{"key":"99_CR19","doi-asserted-by":"publisher","first-page":"764","DOI":"10.1038\/nature03956","volume":"437","author":"GE Nybakken","year":"2005","unstructured":"Nybakken, G. E. et al. Structural basis of West Nile virus neutralization by a therapeutic antibody. Nature 437, 764\u2013769 (2005).","journal-title":"Nature"},{"key":"99_CR20","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1038\/nm1240","volume":"11","author":"T Oliphant","year":"2005","unstructured":"Oliphant, T. et al. Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus. Nat. Med. 11, 522\u2013530 (2005).","journal-title":"Nat. Med."},{"key":"99_CR21","doi-asserted-by":"publisher","first-page":"1016","DOI":"10.1016\/j.cell.2016.07.020","volume":"166","author":"HY Zhao","year":"2016","unstructured":"Zhao, H. Y. et al. Structural basis of Zika virus-specific antibody protection. Cell 166, 1016\u20131027 (2016).","journal-title":"Cell"},{"key":"99_CR22","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1038\/nature18938","volume":"536","author":"G Barba-Spaeth","year":"2016","unstructured":"Barba-Spaeth, G. et al. Structural basis of potent Zika-dengue virus antibody cross-neutralization. Nature 536, 48\u201353 (2016).","journal-title":"Nature"},{"key":"99_CR23","doi-asserted-by":"publisher","first-page":"18950","DOI":"10.1073\/pnas.1011036107","volume":"107","author":"B Kaufmann","year":"2010","unstructured":"Kaufmann, B. et al. Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354. Proc. Natl Acad. Sci. USA 107, 18950\u201318955 (2010).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"99_CR24","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1007\/s13238-014-0087-3","volume":"5","author":"M Dang","year":"2014","unstructured":"Dang, M. et al. Molecular mechanism of SCARB2-mediated attachment and uncoating of EV71. Protein Cell 5, 692\u2013703 (2014).","journal-title":"Protein Cell"},{"key":"99_CR25","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1038\/nature08280","volume":"460","author":"G Orso","year":"2009","unstructured":"Orso, G. et al. Homotypic fusion of ER membranes requires the dynamin-like GTPase atlastin. Nature 460, 978\u2013983 (2009).","journal-title":"Nature"},{"key":"99_CR26","doi-asserted-by":"crossref","unstructured":"Minor, W. & Otwinowski, Z. in Methods in Enzymology, Macromolecular Crystallography (ed. Carter, C. W. Jr) 307\u2013326 (Academic Press, New York, 1997); https:\/\/doi.org\/10.1016\/S0076-6879(97)76066-X.","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"99_CR27","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1073\/pnas.1616502114","volume":"114","author":"X Wang","year":"2017","unstructured":"Wang, X. et al. Potent neutralization of hepatitis A virus reveals a receptor mimic mechanism and the receptor recognition site. Proc. Natl Acad. Sci. USA 114, 770\u2013775 (2017).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"99_CR28","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"AJ McCoy","year":"2007","unstructured":"McCoy, A. J. et al. Phaser crystallographic software. J. Appl. Crystallogr. 40, 658\u2013674 (2007).","journal-title":"J. Appl. Crystallogr."},{"key":"99_CR29","doi-asserted-by":"publisher","first-page":"2126","DOI":"10.1107\/S0907444904019158","volume":"60","author":"P Emsley","year":"2004","unstructured":"Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D 60, 2126\u20132132 (2004).","journal-title":"Acta Crystallogr. D"},{"key":"99_CR30","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.jsb.2005.07.007","volume":"152","author":"DN Mastronarde","year":"2005","unstructured":"Mastronarde, D. N. Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36\u201351 (2005).","journal-title":"J. Struct. Biol."},{"key":"99_CR31","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/nmeth.2472","volume":"10","author":"X Li","year":"2013","unstructured":"Li, X. et al. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat. Methods 10, 584\u2013590 (2013).","journal-title":"Nat. Methods"},{"key":"99_CR32","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1006\/jsbi.1999.4174","volume":"128","author":"SJ Ludtke","year":"1999","unstructured":"Ludtke, S. J., Baldwin, P. R. & Chiu, W. EMAN: semiautomated software for high-resolution single-particle reconstructions. J. Struct. Biol. 128, 82\u201397 (1999).","journal-title":"J. Struct. Biol."},{"key":"99_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jsb.2015.11.003","volume":"193","author":"K Zhang","year":"2016","unstructured":"Zhang, K. Gctf: real-time CTF determination and correction. J. Struct. Biol. 193, 1\u201312 (2016).","journal-title":"J. Struct. Biol."},{"key":"99_CR34","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.jsb.2012.09.006","volume":"180","author":"SH Scheres","year":"2012","unstructured":"Scheres, S. H. RELION: implementation of a Bayesian approach to cryo-EM structure determination. J. Struct. Biol. 180, 519\u2013530 (2012).","journal-title":"J. Struct. Biol."},{"key":"99_CR35","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.jsb.2006.05.009","volume":"157","author":"G Tang","year":"2007","unstructured":"Tang, G. et al. EMAN2: an extensible image processing suite for electron microscopy. J. Struct. Biol. 157, 38\u201346 (2007).","journal-title":"J. Struct. Biol."},{"key":"99_CR36","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"EF Pettersen","year":"2004","unstructured":"Pettersen, E. F. et al. UCSF Chimera\u2014A visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605\u20131612 (2004).","journal-title":"J. Comput. Chem."},{"key":"99_CR37","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1107\/S0907444909042073","volume":"66","author":"VB Chen","year":"2009","unstructured":"Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr. D 66, 12\u201321 (2009).","journal-title":"Acta Crystallogr. D"},{"key":"99_CR38","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1038\/nmeth.3541","volume":"12","author":"BA Barad","year":"2015","unstructured":"Barad, B. A. et al. EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy. Nat. Methods 12, 943\u2013946 (2015).","journal-title":"Nat. Methods"},{"key":"99_CR39","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method. Methods 25, 402\u2013408 (2001).","journal-title":"Methods"},{"key":"99_CR40","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.jsb.2006.10.013","volume":"158","author":"C Xiao","year":"2007","unstructured":"Xiao, C. & Rossmann, M. G. Interpretation of electron density with stereographic roadmap projections. J. Struct. Biol. 158, 182\u2013187 (2007).","journal-title":"J. Struct. Biol."}],"container-title":["Nature Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41564-017-0099-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41564-017-0099-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41564-017-0099-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T23:46:55Z","timestamp":1671580015000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41564-017-0099-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,29]]},"references-count":40,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,3]]}},"alternative-id":["99"],"URL":"https:\/\/doi.org\/10.1038\/s41564-017-0099-x","relation":{},"ISSN":["2058-5276"],"issn-type":[{"value":"2058-5276","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,29]]},"assertion":[{"value":"24 August 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 December 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 January 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}