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Interferon (IFN)-\u03b3-producing CD8<jats:sup>+<\/jats:sup> T cells contribute to control of <jats:italic>Mtb<\/jats:italic> infection, in part by promoting antimicrobial activities of macrophages. Whether <jats:italic>Mtb<\/jats:italic> counters these responses, particularly during hypoxic conditions, remains unknown. Using metabolomic, proteomic and genetic approaches, here we show that <jats:italic>Mtb<\/jats:italic> induced Rv0884c (SerC), an <jats:italic>Mtb<\/jats:italic> phosphoserine aminotransferase, to produce <jats:sc>d<\/jats:sc>-serine. This activity increased <jats:italic>Mtb<\/jats:italic> pathogenesis in mice but did not directly affect intramacrophage <jats:italic>Mtb<\/jats:italic> survival. Instead, <jats:sc>d<\/jats:sc>-serine inhibited IFN-\u03b3 production by CD8<jats:sup>+<\/jats:sup> T cells, which indirectly reduced the ability of macrophages to restrict <jats:italic>Mtb<\/jats:italic> upon co-culture. Mechanistically, <jats:sc>d<\/jats:sc>-serine interacted with WDR24 and inhibited mTORC1 activation in CD8<jats:sup>+<\/jats:sup> T cells. This decreased T-bet expression and reduced IFN-\u03b3 production by CD8<jats:sup>+<\/jats:sup> T cells. 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