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Here we used engineering biology to stably integrate pSOL1 into the chromosome together with a synthetic isopropanol pathway. In a membrane bioreactor continuously fed with glucose mineral medium, the final strain produced advanced biofuels,\n                    <jats:italic>n<\/jats:italic>\n                    -butanol and isopropanol, at high yield (0.31\u2009g\u2009g\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), titre (15.4\u2009g\u2009l\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and productivity (15.5\u2009g\u2009l\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) without degeneration.\n                  <\/jats:p>","DOI":"10.1038\/s41564-024-01714-w","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T06:04:10Z","timestamp":1718345050000},"page":"1655-1660","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production"],"prefix":"10.1038","volume":"9","author":[{"given":"Muhammad","family":"Ehsaan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minyeong","family":"Yoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1105-472X","authenticated-orcid":false,"given":"Wouter","family":"Kuit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Foulquier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1724-7136","authenticated-orcid":false,"given":"Philippe","family":"Soucaille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9277-1261","authenticated-orcid":false,"given":"Nigel P.","family":"Minton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,14]]},"reference":[{"key":"1714_CR1","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1128\/mr.50.4.484-524.1986","volume":"50","author":"DT Jones","year":"1986","unstructured":"Jones, D. 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