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The BAM is structurally conserved, but most studies have focused on Gammaproteobacteria. Here, using single-particle cryogenic electron microscopy, quantitative proteomics and functional assays, we show that the BAM complex is distinct within the Bacteroidota. Cryogenic electron microscopy structures of BAM complexes from the human gut symbiont\n                    <jats:italic>Bacteroides thetaiotaomicron<\/jats:italic>\n                    (3.3\u2009\u00c5) and the human oral pathogen\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    (3.2\u2009\u00c5) show similar, seven-component complexes of ~325\u2009kDa. The complexes are mostly extracellular and comprise canonical BamA and BamD; an integral, essential outer membrane protein, BamG, that associates with BamA; and four surface-exposed lipoproteins: BamH\u2013K. Absent from the BAM in Pseudomonadota, BamG\u2013K form a large, extracellular dome that may confer additional functionality to enable the folding and assembly of \u03b2-barrel\u2013surface-exposed lipoprotein complexes that are a hallmark of the Bacteroidota. Our findings develop our understanding of fundamental biological processes in an important bacterial phylum.\n                  <\/jats:p>","DOI":"10.1038\/s41564-025-02132-2","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:04:38Z","timestamp":1759331078000},"page":"2845-2859","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Structure of a distinct \u03b2-barrel assembly machinery complex in the Bacteroidota"],"prefix":"10.1038","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3338-2087","authenticated-orcid":false,"given":"Augustinas","family":"Silale","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariusz","family":"Madej","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6118-489X","authenticated-orcid":false,"given":"Katarzyna","family":"Mikruta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5194-447X","authenticated-orcid":false,"given":"Andrew M.","family":"Frey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4092-2132","authenticated-orcid":false,"given":"Adam J.","family":"Hart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Basl\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4304-0681","authenticated-orcid":false,"given":"Carsten","family":"Scavenius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9292-9172","authenticated-orcid":false,"given":"Jan J.","family":"Enghild","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5732-700X","authenticated-orcid":false,"given":"Matthias","family":"Trost","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P.","family":"Hirt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1966-8804","authenticated-orcid":false,"given":"Bert","family":"van den Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,1]]},"reference":[{"key":"2132_CR1","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1126\/science.aad3460","volume":"351","author":"J Bakelar","year":"2016","unstructured":"Bakelar, J., Buchanan, S. 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