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Here we report that\n                    <jats:italic>Plectosphaerella cucumerina<\/jats:italic>\n                    is a core member of the\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    root microbiota, which displays pathogenic potential across multiple hosts. Using a collection of 72\n                    <jats:italic>Plectosphaerella<\/jats:italic>\n                    isolates and whole-genome sequencing, we observed subtle phenotypic and genotypic variation associated with fungal phylogeny but not host plant identity. Transcriptome profiling of a\n                    <jats:italic>P. cucumerina<\/jats:italic>\n                    isolate in roots of diverse plants revealed core and host-specific fungal responses, including induction of carbohydrate-active enzymes (CAZymes) involved in root cell wall deconstruction. A fungal gene encoding a candidate \u03b2-1,3-glucanase (GH64) was identified as a key genetic factor driving multihost infection. This gene is present across plant-colonizing fungi and functions as a disease determinant in both\n                    <jats:italic>P. cucumerina<\/jats:italic>\n                    and a\n                    <jats:italic>Colletotrichum<\/jats:italic>\n                    root pathogen. Our results indicate that host-induced CAZymes can couple fungal virulence with multihost compatibility.\n                  <\/jats:p>","DOI":"10.1038\/s41564-026-02492-3","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:02:36Z","timestamp":1790845356000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Carbohydrate-active enzymes from a core root mycobiota member enable infection of multiple plant hosts"],"prefix":"10.1038","author":[{"given":"Ram-Sevak","family":"Raja-Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3044-1398","authenticated-orcid":false,"given":"Fantin","family":"Mesny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arpan 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