{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T23:38:20Z","timestamp":1794008300815,"version":"4.8.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"7816","license":[{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2020,7,16]]},"DOI":"10.1038\/s41586-020-2332-7","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T06:03:18Z","timestamp":1589436198000},"page":"469-472","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":952,"title":["Proteomics of SARS-CoV-2-infected host cells reveals therapy targets"],"prefix":"10.1038","volume":"583","author":[{"given":"Denisa","family":"Bojkova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2276-8128","authenticated-orcid":false,"given":"Kevin","family":"Klann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3253-8396","authenticated-orcid":false,"given":"Benjamin","family":"Koch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5417-9307","authenticated-orcid":false,"given":"Marek","family":"Widera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Krause","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1802-7565","authenticated-orcid":false,"given":"Sandra","family":"Ciesek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6744-2087","authenticated-orcid":false,"given":"Jindrich","family":"Cinatl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3832-090X","authenticated-orcid":false,"given":"Christian","family":"M\u00fcnch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,14]]},"reference":[{"key":"2332_CR1","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1056\/NEJMoa2001017","volume":"382","author":"N Zhu","year":"2020","unstructured":"Zhu, N. et al. A novel coronavirus from patients with pneumonia in China, 2019. N. Engl. J. Med. 382, 727\u2013733 (2020).","journal-title":"N. Engl. J. Med."},{"key":"2332_CR2","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.ijid.2020.01.050","volume":"92","author":"S Zhao","year":"2020","unstructured":"Zhao, S. et al. Preliminary estimation of the basic reproduction number of novel coronavirus (2019-nCoV) in China, from 2019 to 2020: a data-driven analysis in the early phase of the outbreak. Int. J. Infect. Dis. 92, 214\u2013217 (2020).","journal-title":"Int. J. Infect. Dis."},{"key":"2332_CR3","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/j.molcel.2019.11.010","volume":"77","author":"K Klann","year":"2020","unstructured":"Klann, K., Tascher, G. & M\u00fcnch, C. Functional translatome proteomics reveal converging and dose-dependent regulation by mTORC1 and eIF2\u03b1. Mol. Cell 77, 913\u2013925 (2020).","journal-title":"Mol. Cell"},{"key":"2332_CR4","first-page":"4","volume":"7","author":"Y-H Jin","year":"2020","unstructured":"Jin, Y.-H. et al. A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version). Mil. Med. Res. 7, 4 (2020).","journal-title":"Mil. Med. Res."},{"key":"2332_CR5","volume":"9","author":"J She","year":"2020","unstructured":"She, J. et al. 2019 novel coronavirus of pneumonia in Wuhan, China: emerging attack and management strategies. Clin. Transl. Med. 9, 19 (2020).","journal-title":"Clin. Transl. Med."},{"key":"2332_CR6","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1056\/NEJMc2001899","volume":"382","author":"S Hoehl","year":"2020","unstructured":"Hoehl, S. et al. Evidence of SARS-CoV-2 infection in returning travelers from Wuhan, China. N. Engl. J. Med. 382, 1278\u20131280 (2020).","journal-title":"N. Engl. J. Med."},{"key":"2332_CR7","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1053\/j.gastro.2020.02.055","volume":"158","author":"F Xiao","year":"2020","unstructured":"Xiao, F. et al. Evidence for gastrointestinal infection of SARS-CoV-2. Gastroenterology 158, 1831\u20131833 (2020).","journal-title":"Gastroenterology"},{"key":"2332_CR8","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1001\/jama.2020.3204","volume":"323","author":"BE Young","year":"2020","unstructured":"Young, B. E. et al. Epidemiologic features and clinical course of patients infected with SARS-CoV-2 in Singapore. J. Am. Med. Assoc. 323, 1488\u20131494 (2020).","journal-title":"J. Am. Med. Assoc."},{"key":"2332_CR9","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0140-6736(03)13973-6","volume":"362","author":"J Cinatl","year":"2003","unstructured":"Cinatl, J. et al. Treatment of SARS with human interferons. Lancet 362, 293\u2013294 (2003).","journal-title":"Lancet"},{"key":"2332_CR10","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1038\/nsmb.1680","volume":"16","author":"W Kamitani","year":"2009","unstructured":"Kamitani, W., Huang, C., Narayanan, K., Lokugamage, K. G. & Makino, S. A two-pronged strategy to suppress host protein synthesis by SARS coronavirus Nsp1 protein. Nat. Struct. Mol. Biol. 16, 1134\u20131140 (2009).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"2332_CR11","doi-asserted-by":"crossref","first-page":"e00023","DOI":"10.1128\/JVI.00023-19","volume":"93","author":"L Shen","year":"2019","unstructured":"Shen, L. et al. High-throughput screening and identification of potent broad-spectrum inhibitors of coronaviruses. J. Virol. 93, e00023-19 (2019).","journal-title":"J. Virol."},{"key":"2332_CR12","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.drudis.2020.01.015","volume":"25","author":"T Pillaiyar","year":"2020","unstructured":"Pillaiyar, T., Meenakshisundaram, S. & Manickam, M. Recent discovery and development of inhibitors targeting coronaviruses. Drug Discov. Today 25, 668\u2013688 (2020).","journal-title":"Drug Discov. Today"},{"key":"2332_CR13","doi-asserted-by":"crossref","first-page":"5279","DOI":"10.1128\/JVI.02631-07","volume":"82","author":"BW Neuman","year":"2008","unstructured":"Neuman, B. W. et al. Proteomics analysis unravels the functional repertoire of coronavirus nonstructural protein 3. J. Virol. 82, 5279\u20135294 (2008).","journal-title":"J. Virol."},{"key":"2332_CR14","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1002\/pmic.201100469","volume":"12","author":"SS Jourdan","year":"2012","unstructured":"Jourdan, S. S., Osorio, F. & Hiscox, J. A. An interactome map of the nucleocapsid protein from a highly pathogenic North American porcine reproductive and respiratory syndrome virus strain generated using SILAC-based quantitative proteomics. Proteomics 12, 1015\u20131023 (2012).","journal-title":"Proteomics"},{"key":"2332_CR15","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.jprot.2016.05.009","volume":"142","author":"T Song","year":"2016","unstructured":"Song, T. et al. Quantitative interactome reveals that porcine reproductive and respiratory syndrome virus nonstructural protein 2 forms a complex with viral nucleocapsid protein and cellular vimentin. J. Proteomics 142, 70\u201381 (2016).","journal-title":"J. Proteomics"},{"key":"2332_CR16","doi-asserted-by":"crossref","first-page":"9486","DOI":"10.1128\/JVI.00321-13","volume":"87","author":"E Emmott","year":"2013","unstructured":"Emmott, E. et al. The cellular interactome of the coronavirus infectious bronchitis virus nucleocapsid protein and functional implications for virus biology. J. Virol. 87, 9486\u20139500 (2013).","journal-title":"J. Virol."},{"key":"2332_CR17","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.molcel.2018.03.011","volume":"70","author":"C Cretu","year":"2018","unstructured":"Cretu, C. et al. Structural basis of splicing modulation by antitumor macrolide compounds. Mol. Cell 70, 265\u2013273 (2018).","journal-title":"Mol. Cell"},{"key":"2332_CR18","doi-asserted-by":"crossref","first-page":"E7158","DOI":"10.1073\/pnas.1800525115","volume":"115","author":"GA Gualdoni","year":"2018","unstructured":"Gualdoni, G. A. et al. Rhinovirus induces an anabolic reprogramming in host cell metabolism essential for viral replication. Proc. Natl. Acad. Sci. USA 115, E7158\u2013E7165 (2018).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2332_CR19","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3390\/v11010073","volume":"11","author":"B Yan","year":"2019","unstructured":"Yan, B. et al. Characterization of the lipidomic profile of human coronavirus-infected cells: implications for lipid metabolism remodeling upon coronavirus replication. Viruses 11, 73 (2019).","journal-title":"Viruses"},{"key":"2332_CR20","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.coviro.2019.04.002","volume":"35","author":"AJ Pruijssers","year":"2019","unstructured":"Pruijssers, A. J. & Denison, M. R. Nucleoside analogues for the treatment of coronavirus infections. Curr. Opin. Virol. 35, 57\u201362 (2019).","journal-title":"Curr. Opin. Virol."},{"key":"2332_CR21","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.antiviral.2005.01.003","volume":"66","author":"M Saijo","year":"2005","unstructured":"Saijo, M. et al. Inhibitory effect of mizoribine and ribavirin on the replication of severe acute respiratory syndrome (SARS)-associated coronavirus. Antiviral Res. 66, 159\u2013163 (2005).","journal-title":"Antiviral Res."},{"key":"2332_CR22","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1128\/AAC.04618-14","volume":"59","author":"LS Wu","year":"2015","unstructured":"Wu, L. S. et al. Population pharmacokinetic modeling of plasma and intracellular ribavirin concentrations in patients with chronic hepatitis c virus infection. Antimicrob. Agents Chemother. 59, 2179\u20132188 (2015).","journal-title":"Antimicrob. Agents Chemother."},{"key":"2332_CR23","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/s41422-020-0282-0","volume":"30","author":"M Wang","year":"2020","unstructured":"Wang, M. et al. Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. Cell Res. 30, 269\u2013271 (2020).","journal-title":"Cell Res."},{"key":"2332_CR24","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1016\/S0140-6736(03)13615-X","volume":"361","author":"J Cinatl","year":"2003","unstructured":"Cinatl, J. et al. Glycyrrhizin, an active component of liquorice roots, and replication of SARS-associated coronavirus. Lancet 361, 2045\u20132046 (2003).","journal-title":"Lancet"},{"key":"2332_CR25","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1111\/j.1440-1746.2008.05398.x","volume":"23","author":"P Martin","year":"2008","unstructured":"Martin, P. & Jensen, D. M. Ribavirin in the treatment of chronic hepatitis C. J. Gastroenterol. Hepatol. 23, 844\u2013855 (2008).","journal-title":"J. Gastroenterol. Hepatol."},{"key":"2332_CR26","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1111\/tid.12194","volume":"16","author":"JR Marcelin","year":"2014","unstructured":"Marcelin, J. R., Wilson, J. W. & Razonable, R. R. Oral ribavirin therapy for respiratory syncytial virus infections in moderately to severely immunocompromised patients. Transpl. Infect. Dis. 16, 242\u2013250 (2014).","journal-title":"Transpl. Infect. Dis."},{"key":"2332_CR27","doi-asserted-by":"crossref","first-page":"39","DOI":"10.3389\/fmolb.2017.00039","volume":"4","author":"Y Ye","year":"2017","unstructured":"Ye, Y., Tang, W. K., Zhang, T. & Xia, D. A mighty \u201cprotein extractor\u201d of the cell: structure and function of the p97\/CDC48 ATPase. Front. Mol. Biosci. 4, 39 (2017).","journal-title":"Front. Mol. Biosci."},{"key":"2332_CR28","first-page":"86","volume":"133","author":"J Zhang","year":"2019","unstructured":"Zhang, J. et al. Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses. Eur. J. Pharm. Sci. 133, 86\u201394 (2019).","journal-title":"J. Pharm. Sci."},{"key":"2332_CR29","doi-asserted-by":"crossref","first-page":"L84","DOI":"10.1152\/ajplung.00071.2009","volume":"297","author":"HP Jia","year":"2009","unstructured":"Jia, H. P. et al. Ectodomain shedding of angiotensin converting enzyme 2 in human airway epithelia. Am. J. Physiol. Lung Cell. Mol. Physiol. 297, L84\u2013L96 (2009).","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"2332_CR30","doi-asserted-by":"crossref","first-page":"4471","DOI":"10.1128\/JVI.02472-07","volume":"82","author":"K Narayanan","year":"2008","unstructured":"Narayanan, K. et al. Severe acute respiratory syndrome coronavirus nsp1 suppresses host gene expression, including that of type I interferon, in infected cells. J. Virol. 82, 4471\u20134479 (2008).","journal-title":"J. Virol."},{"key":"2332_CR31","first-page":"2000045","volume":"25","author":"VM Corman","year":"2020","unstructured":"Corman, V. M. et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 25, 2000045 (2020).","journal-title":"Euro Surveill."},{"key":"2332_CR32","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1074\/mcp.M116.065524","volume":"16","author":"DL Plubell","year":"2017","unstructured":"Plubell, D. L. et al. Extended multiplexing of tandem mass tags (TMT) labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue. Mol. Cell. Proteomics 16, 873\u2013890 (2017).","journal-title":"Mol. Cell. Proteomics"},{"key":"2332_CR33","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2010-11-12-144","volume":"11","author":"MD Robinson","year":"2010","unstructured":"Robinson, M. D. & Oshlack, A. A scaling normalization method for differential expression analysis of RNA-seq data. Genome Biol. 11, R25 (2010).","journal-title":"Genome Biol."},{"key":"2332_CR34","doi-asserted-by":"crossref","unstructured":"McKinney, W. Data structures for statistical computing in Python. In Proc. 9th Python in Science Conference 56\u201361 (SCIPY, 2010).","DOI":"10.25080\/Majora-92bf1922-00a"},{"key":"2332_CR35","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1109\/MCSE.2011.37","volume":"13","author":"S van der Walt","year":"2011","unstructured":"van der Walt, S., Colbert, S. C. & Varoquaux, G. The NumPy Array: a structure for efficient numerical computation. Comput. Sci. Eng. 13, 22\u201330 (2011).","journal-title":"Comput. Sci. Eng."},{"key":"2332_CR36","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","volume":"13","author":"S Tyanova","year":"2016","unstructured":"Tyanova, S. et al. The Perseus computational platform for comprehensive analysis of (prote)omics data. Nat. Methods 13, 731\u2013740 (2016).","journal-title":"Nat. Methods"},{"key":"2332_CR37","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","volume":"13","author":"P Shannon","year":"2003","unstructured":"Shannon, P. et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 13, 2498\u20132504 (2003).","journal-title":"Genome Res."},{"key":"2332_CR38","doi-asserted-by":"crossref","first-page":"3448","DOI":"10.1093\/bioinformatics\/bti551","volume":"21","author":"S Maere","year":"2005","unstructured":"Maere, S., Heymans, K. & Kuiper, M. BiNGO: a Cytoscape plugin to assess overrepresentation of gene ontology categories in biological networks. Bioinformatics 21, 3448\u20133449 (2005).","journal-title":"Bioinformatics"},{"key":"2332_CR39","doi-asserted-by":"crossref","first-page":"e13984","DOI":"10.1371\/journal.pone.0013984","volume":"5","author":"D Merico","year":"2010","unstructured":"Merico, D., Isserlin, R., Stueker, O., Emili, A. & Bader, G. D. Enrichment map: a network-based method for gene-set enrichment visualization and interpretation. PLoS ONE 5, e13984 (2010).","journal-title":"PLoS ONE"},{"key":"2332_CR40","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/978-1-4939-6783-4_11","volume":"1558","author":"G Wu","year":"2017","unstructured":"Wu, G. & Haw, R. Functional interaction network construction and analysis for disease discovery. Methods Mol. Biol. 1558, 235\u2013253 (2017).","journal-title":"Methods Mol. Biol."},{"key":"2332_CR41","doi-asserted-by":"crossref","first-page":"D442","DOI":"10.1093\/nar\/gky1106","volume":"47","author":"Y Perez-Riverol","year":"2019","unstructured":"Perez-Riverol, Y. et al. The PRIDE database and related tools and resources in 2019: improving support for quantification data. Nucleic Acids Res. 47, D442\u2013D450 (2019).","journal-title":"Nucleic Acids Res."},{"key":"2332_CR42","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s00705-009-0323-y","volume":"154","author":"J-Y Chen","year":"2009","unstructured":"Chen, J.-Y. et al. Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems. Arch. Virol. 154, 507\u2013512 (2009).","journal-title":"Arch. Virol."},{"key":"2332_CR43","doi-asserted-by":"crossref","first-page":"11116","DOI":"10.1128\/JVI.01360-15","volume":"89","author":"H-H Wong","year":"2015","unstructured":"Wong, H.-H. et al. Genome-wide screen reveals valosin-containing protein requirement for coronavirus exit from endosomes. J. Virol. 89, 11116\u201311128 (2015).","journal-title":"J. Virol."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/s41586-020-2332-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41586-020-2332-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41586-020-2332-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T18:05:23Z","timestamp":1684605923000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41586-020-2332-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,14]]},"references-count":43,"journal-issue":{"issue":"7816","published-print":{"date-parts":[[2020,7,16]]}},"alternative-id":["2332"],"URL":"https:\/\/doi.org\/10.1038\/s41586-020-2332-7","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.737945945.793574871","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.737945945.793574515","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.737945945.793579073","asserted-by":"object"}],"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-17218\/v1","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,14]]},"assertion":[{"value":"27 February 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 May 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 May 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}