{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:51:39Z","timestamp":1793296299837,"version":"4.5.0"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"7851","license":[{"start":{"date-parts":[[2021,3,17]],"date-time":"2021-03-17T00:00:00Z","timestamp":1615939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2021,3,17]],"date-time":"2021-03-17T00:00:00Z","timestamp":1615939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2021,3,25]]},"DOI":"10.1038\/s41586-021-03372-y","type":"journal-article","created":{"date-parts":[[2021,3,17]],"date-time":"2021-03-17T13:02:46Z","timestamp":1615986166000},"page":"627-632","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":357,"title":["Modelling human blastocysts by reprogramming fibroblasts into iBlastoids"],"prefix":"10.1038","volume":"591","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9315-3406","authenticated-orcid":false,"given":"Xiaodong","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia Ping","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5270-0605","authenticated-orcid":false,"given":"Jan","family":"Schr\u00f6der","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Aberkane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1239-1577","authenticated-orcid":false,"given":"John F.","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Mohenska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue Mei","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0863-2769","authenticated-orcid":false,"given":"Yu B. Y.","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guizhi","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichen","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6674-8420","authenticated-orcid":false,"given":"Daniel","family":"Poppe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6637-7239","authenticated-orcid":false,"given":"Ryan","family":"Lister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amander T.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4390-0872","authenticated-orcid":false,"given":"Owen J. L.","family":"Rackham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Zenker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2531-778X","authenticated-orcid":false,"given":"Jose M.","family":"Polo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,17]]},"reference":[{"key":"3372_CR1","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.stem.2016.12.004","volume":"20","author":"J Rossant","year":"2017","unstructured":"Rossant, J. & Tam, P. P. L. New insights into early human development: lessons for stem cell derivation and differentiation. Cell Stem Cell 20, 18\u201328 (2017).","journal-title":"Cell Stem Cell"},{"key":"3372_CR2","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1126\/science.282.5391.1145","volume":"282","author":"JA Thomson","year":"1998","unstructured":"Thomson, J. A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145\u20131147 (1998).","journal-title":"Science"},{"key":"3372_CR3","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1016\/j.cell.2007.11.019","volume":"131","author":"K Takahashi","year":"2007","unstructured":"Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861\u2013872 (2007).","journal-title":"Cell"},{"key":"3372_CR4","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1038\/s41563-020-00829-9","volume":"20","author":"J Fu","year":"2021","unstructured":"Fu, J., Warmflash, A. & Lutolf, M. P. Stem-cell-based embryo models for fundamental research and translation. Nat. Mater. 20, 132\u2013144 (2021).","journal-title":"Nat. Mater."},{"key":"3372_CR5","doi-asserted-by":"publisher","first-page":"eaal1810","DOI":"10.1126\/science.aal1810","volume":"356","author":"SE Harrison","year":"2017","unstructured":"Harrison, S. E., Sozen, B., Christodoulou, N., Kyprianou, C. & Zernicka-Goetz, M. Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro. Science 356, eaal1810 (2017).","journal-title":"Science"},{"key":"3372_CR6","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1038\/s41556-018-0147-7","volume":"20","author":"B Sozen","year":"2018","unstructured":"Sozen, B. et al. Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures. Nat. Cell Biol. 20, 979\u2013989 (2018).","journal-title":"Nat. Cell Biol."},{"key":"3372_CR7","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1038\/s41586-018-0051-0","volume":"557","author":"NC Rivron","year":"2018","unstructured":"Rivron, N. C. et al. Blastocyst-like structures generated solely from stem cells. Nature 557, 106\u2013111 (2018).","journal-title":"Nature"},{"key":"3372_CR8","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08378-9","volume":"10","author":"S Zhang","year":"2019","unstructured":"Zhang, S. et al. Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Nat. Commun. 10, 496 (2019).","journal-title":"Nat. Commun."},{"key":"3372_CR9","doi-asserted-by":"publisher","first-page":"698","DOI":"10.1016\/j.devcel.2019.11.014","volume":"51","author":"B Sozen","year":"2019","unstructured":"Sozen, B. et al. Self-organization of mouse stem cells into an extended potential blastoid. Dev. Cell 51, 698\u2013712 (2019).","journal-title":"Dev. Cell"},{"key":"3372_CR10","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/j.cell.2019.09.029","volume":"179","author":"R Li","year":"2019","unstructured":"Li, R. et al. Generation of blastocyst-like structures from mouse embryonic and adult cell cultures. Cell 179, 687\u2013702 (2019).","journal-title":"Cell"},{"key":"3372_CR11","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1038\/s41586-020-2734-6","volume":"586","author":"X Liu","year":"2020","unstructured":"Liu, X. et al. Reprogramming roadmap reveals route to human induced trophoblast stem cells. Nature 586, 101\u2013107 (2020).","journal-title":"Nature"},{"key":"3372_CR12","doi-asserted-by":"publisher","first-page":"3613","DOI":"10.1242\/dev.131235","volume":"142","author":"P Blakeley","year":"2015","unstructured":"Blakeley, P. et al. Defining the three cell lineages of the human blastocyst by single-cell RNA-seq. Development 142, 3613 (2015).","journal-title":"Development"},{"key":"3372_CR13","doi-asserted-by":"publisher","first-page":"1012","DOI":"10.1016\/j.cell.2016.03.023","volume":"165","author":"S Petropoulos","year":"2016","unstructured":"Petropoulos, S. et al. Single-cell RNA-seq reveals lineage and X chromosome dynamics in human preimplantation embryos. Cell 165, 1012\u20131026 (2016).","journal-title":"Cell"},{"key":"3372_CR14","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1038\/ncb3347","volume":"18","author":"MN Shahbazi","year":"2016","unstructured":"Shahbazi, M. N. et al. Self-organization of the human embryo in the absence of maternal tissues. Nat. Cell Biol. 18, 700\u2013708 (2016).","journal-title":"Nat. Cell Biol."},{"key":"3372_CR15","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1038\/s41586-019-1875-y","volume":"577","author":"L Xiang","year":"2020","unstructured":"Xiang, L. et al. A developmental landscape of 3D-cultured human pre-gastrulation embryos. Nature 577, 537\u2013542 (2020).","journal-title":"Nature"},{"key":"3372_CR16","doi-asserted-by":"publisher","first-page":"2301","DOI":"10.1016\/j.celrep.2016.02.036","volume":"14","author":"H Qin","year":"2016","unstructured":"Qin, H. et al. YAP induces human naive pluripotency. Cell Rep. 14, 2301\u20132312 (2016).","journal-title":"Cell Rep."},{"key":"3372_CR17","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-08244-0","volume":"10","author":"L Liu","year":"2019","unstructured":"Liu, L. et al. An integrated chromatin accessibility and transcriptome landscape of human pre-implantation embryos. Nat. Commun. 10, 364 (2019).","journal-title":"Nat. Commun."},{"key":"3372_CR18","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.devcel.2016.06.014","volume":"38","author":"J Durruthy-Durruthy","year":"2016","unstructured":"Durruthy-Durruthy, J. et al. Spatiotemporal reconstruction of the human blastocyst by single-cell gene-expression analysis informs induction of naive pluripotency. Dev. Cell 38, 100\u2013115 (2016).","journal-title":"Dev. Cell"},{"key":"3372_CR19","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1038\/nature24033","volume":"550","author":"NME Fogarty","year":"2017","unstructured":"Fogarty, N. M. E. et al. Genome editing reveals a role for OCT4 in human embryogenesis. Nature 550, 67\u201373 (2017).","journal-title":"Nature"},{"key":"3372_CR20","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.ydbio.2012.12.008","volume":"375","author":"KK Niakan","year":"2013","unstructured":"Niakan, K. K. & Eggan, K. Analysis of human embryos from zygote to blastocyst reveals distinct gene expression patterns relative to the mouse. Dev. Biol. 375, 54\u201364 (2013).","journal-title":"Dev. Biol."},{"key":"3372_CR21","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1038\/nature17948","volume":"533","author":"A Deglincerti","year":"2016","unstructured":"Deglincerti, A. et al. Self-organization of the in vitro attached human embryo. Nature 533, 251\u2013254 (2016).","journal-title":"Nature"},{"key":"3372_CR22","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.ydbio.2011.10.030","volume":"361","author":"M Roode","year":"2012","unstructured":"Roode, M. et al. Human hypoblast formation is not dependent on FGF signalling. Dev. Biol. 361, 358\u2013363 (2012).","journal-title":"Dev. Biol."},{"key":"3372_CR23","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/S1472-6483(10)61650-1","volume":"8","author":"B Kovacic","year":"2004","unstructured":"Kovacic, B., Vlaisavljevic, V., Reljic, M. & Cizek-Sajko, M. Developmental capacity of different morphological types of day 5 human morulae and blastocysts. Reprod. Biomed. Online 8, 687\u2013694 (2004).","journal-title":"Reprod. Biomed. Online"},{"key":"3372_CR24","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1038\/s41556-020-00609-2","volume":"23","author":"E Posfai","year":"2021","unstructured":"Posfai, E. et al. Evaluating totipotency using criteria of increasing stringency. Nat. Cell Biol. 23, 49\u201360 (2021).","journal-title":"Nat. Cell Biol."},{"key":"3372_CR25","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.stem.2017.11.004","volume":"22","author":"H Okae","year":"2018","unstructured":"Okae, H. et al. Derivation of human trophoblast stem cells. Cell Stem Cell 22, 50\u201363 (2018).","journal-title":"Cell Stem Cell"},{"key":"3372_CR26","doi-asserted-by":"publisher","unstructured":"Tyser, R. C. V., Mahammadov, E., Nakanoh, S. & Vallier, L. A spatially resolved single cell atlas of human gastrulation. Preprint at https:\/\/doi.org\/10.1101\/2020.07.21.213512 (2020).","DOI":"10.1101\/2020.07.21.213512"},{"key":"3372_CR27","doi-asserted-by":"publisher","first-page":"4568","DOI":"10.1016\/j.celrep.2019.11.083","volume":"29","author":"D Chen","year":"2019","unstructured":"Chen, D. et al. Human primordial germ cells are specified from lineage-primed progenitors. Cell Rep. 29, 4568\u20134582 (2019).","journal-title":"Cell Rep."},{"key":"3372_CR28","doi-asserted-by":"publisher","first-page":"22635","DOI":"10.1073\/pnas.1911362116","volume":"116","author":"RC West","year":"2019","unstructured":"West, R. C. et al. Dynamics of trophoblast differentiation in peri-implantation-stage human embryos. Proc. Natl Acad. Sci. USA 116, 22635\u201322644 (2019).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"3372_CR29","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1093\/humrep\/der037","volume":"26","author":"Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology","year":"2011","unstructured":"Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum. Reprod. 26, 1270\u20131283 (2011).","journal-title":"Hum. Reprod."},{"key":"3372_CR30","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.stemcr.2019.12.008","volume":"14","author":"I Hyun","year":"2020","unstructured":"Hyun, I., Munsie, M., Pera, M. F., Rivron, N. C. & Rossant, J. Toward guidelines for research on human embryo models formed from stem cells. Stem Cell Rep. 14, 169\u2013174 (2020).","journal-title":"Stem Cell Rep."},{"key":"3372_CR31","unstructured":"Warnock\u2014report of the committee of inquiry into human fertilisation and embryology. Ir. Nurs. News 5, 7\u20138 (1985)."},{"key":"3372_CR32","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/j.stemcr.2016.02.005","volume":"6","author":"G Guo","year":"2016","unstructured":"Guo, G. et al. Naive pluripotent stem cells derived directly from isolated cells of the human inner cell mass. Stem Cell Rep. 6, 437\u2013446 (2016).","journal-title":"Stem Cell Rep."},{"key":"3372_CR33","doi-asserted-by":"publisher","unstructured":"Tan, J. P., Liu, X. & Polo, J. M. Generation of human blastocyst-like structures by somatic cell reprogramming. Protoc. Exch. https:\/\/doi.org\/10.21203\/rs.3.pex-1347\/v1 (2021).","DOI":"10.21203\/rs.3.pex-1347\/v1"},{"key":"3372_CR34","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1038\/nmeth.4436","volume":"14","author":"X Liu","year":"2017","unstructured":"Liu, X. et al. Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming. Nat. Methods 14, 1055\u20131062 (2017).","journal-title":"Nat. Methods"},{"key":"3372_CR35","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1038\/s41586-019-1500-0","volume":"572","author":"F Zhou","year":"2019","unstructured":"Zhou, F. et al. Reconstituting the transcriptome and DNA methylome landscapes of human implantation. Nature 572, 660\u2013664 (2019).","journal-title":"Nature"},{"key":"3372_CR36","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1007\/978-1-4939-9086-3_3","volume":"1940","author":"JM Paynter","year":"2019","unstructured":"Paynter, J. M., Chen, J., Liu, X. & Nefzger, C. M. Propagation and maintenance of mouse embryonic stem cells. Methods Mol. Biol. 1940, 33\u201345 (2019).","journal-title":"Methods Mol. Biol."},{"key":"3372_CR37","doi-asserted-by":"publisher","first-page":"dev172916","DOI":"10.1242\/dev.172916","volume":"146","author":"M Rostovskaya","year":"2019","unstructured":"Rostovskaya, M., Stirparo, G. G. & Smith, A. Capacitation of human na\u00efve pluripotent stem cells for multi-lineage differentiation. Development 146, dev172916 (2019).","journal-title":"Development"},{"key":"3372_CR38","doi-asserted-by":"publisher","first-page":"dev167833","DOI":"10.1242\/dev.167833","volume":"145","author":"T Boroviak","year":"2018","unstructured":"Boroviak, T. et al. Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development. Development 145, dev167833 (2018).","journal-title":"Development"},{"key":"3372_CR39","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1038\/s41586-020-2647-4","volume":"585","author":"HYG Lim","year":"2020","unstructured":"Lim, H. Y. G. et al. Keratins are asymmetrically inherited fate determinants in the mammalian embryo. Nature 585, 404\u2013409 (2020).","journal-title":"Nature"},{"key":"3372_CR40","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1038\/s41586-020-2759-x","volume":"587","author":"C Gerri","year":"2020","unstructured":"Gerri, C. et al. Initiation of a conserved trophectoderm program in human, cow and mouse embryos. Nature 587, 443\u2013447 (2020).","journal-title":"Nature"},{"key":"3372_CR41","first-page":"375","volume":"24","author":"A Aberkane","year":"2018","unstructured":"Aberkane, A. et al. Expression of adhesion and extracellular matrix genes in human blastocysts upon attachment in a 2D co-culture system. Mol. Hum. Reprod. 24, 375\u2013387 (2018).","journal-title":"Mol. Hum. Reprod."},{"key":"3372_CR42","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5","volume":"7","author":"P Bankhead","year":"2017","unstructured":"Bankhead, P. et al. QuPath: Open source software for digital pathology image analysis. Sci. Rep. 7, 16878 (2017).","journal-title":"Sci. Rep."},{"key":"3372_CR43","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1681\/ASN.2013080831","volume":"25","author":"AQ Lam","year":"2014","unstructured":"Lam, A. Q. et al. Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers. J. Am. Soc. Nephrol. 25, 1211\u20131225 (2014).","journal-title":"J. Am. Soc. Nephrol."},{"key":"3372_CR44","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","volume":"29","author":"A Dobin","year":"2013","unstructured":"Dobin, A. et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29, 15\u201321 (2013).","journal-title":"Bioinformatics"},{"key":"3372_CR45","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1865-2","volume":"20","author":"Y Huang","year":"2019","unstructured":"Huang, Y., McCarthy, D. J. & Stegle, O. Vireo: Bayesian demultiplexing of pooled single-cell RNA-seq data without genotype reference. Genome Biol. 20, 273 (2019).","journal-title":"Genome Biol."},{"key":"3372_CR46","doi-asserted-by":"crossref","unstructured":"Wickham, H. et al. dplyr: a grammar of data manipulation. R version 1.0.5 https:\/\/CRAN.R-project.org\/package=dplyr (2021).","DOI":"10.1007\/978-1-4842-6876-6_1"},{"key":"3372_CR47","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1038\/nbt.4096","volume":"36","author":"A Butler","year":"2018","unstructured":"Butler, A., Hoffman, P., Smibert, P., Papalexi, E. & Satija, R. Integrating single-cell transcriptomic data across different conditions, technologies, and species. Nat. Biotechnol. 36, 411\u2013420 (2018).","journal-title":"Nat. Biotechnol."},{"key":"3372_CR48","doi-asserted-by":"publisher","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","volume":"177","author":"T Stuart","year":"2019","unstructured":"Stuart, T. et al. Comprehensive integration of single-cell data. Cell 177, 1888\u20131902 (2019).","journal-title":"Cell"},{"key":"3372_CR49","doi-asserted-by":"crossref","unstructured":"Wickham, H. ggplot2: Elegant Graphics for Data Analysis (Springer, 2016).","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"3372_CR50","doi-asserted-by":"publisher","first-page":"574","DOI":"10.12688\/f1000research.6925.1","volume":"4","author":"R Kolde","year":"2015","unstructured":"Kolde, R. & Vilo, J. GOsummaries: an R package for visual functional annotation of experimental data. F1000Res. 4, 574 (2015).","journal-title":"F1000Res."},{"key":"3372_CR51","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1874-1","volume":"20","author":"C Hafemeister","year":"2019","unstructured":"Hafemeister, C. & Satija, R. Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression. Genome Biol. 20, 296 (2019).","journal-title":"Genome Biol."},{"key":"3372_CR52","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.ymeth.2015.06.021","volume":"85","author":"A Scialdone","year":"2015","unstructured":"Scialdone, A. et al. Computational assignment of cell-cycle stage from single-cell transcriptome data. Methods 85, 54\u201361 (2015).","journal-title":"Methods"},{"key":"3372_CR53","doi-asserted-by":"publisher","first-page":"dev180620","DOI":"10.1242\/dev.180620","volume":"146","author":"M Linneberg-Agerholm","year":"2019","unstructured":"Linneberg-Agerholm, M. et al. Na\u00efve human pluripotent stem cells respond to Wnt, Nodal and LIF signalling to produce expandable na\u00efve extra-embryonic endoderm. Development 146, dev180620 (2019).","journal-title":"Development"}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41586-021-03372-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41586-021-03372-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41586-021-03372-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T09:05:48Z","timestamp":1713344748000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41586-021-03372-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,17]]},"references-count":53,"journal-issue":{"issue":"7851","published-print":{"date-parts":[[2021,3,25]]}},"alternative-id":["3372"],"URL":"https:\/\/doi.org\/10.1038\/s41586-021-03372-y","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.739757252.793587344","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,17]]},"assertion":[{"value":"24 August 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 February 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 March 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Although it is not directly related to this manuscript, O.J.L.R. and J.M.P. are co-inventors on a patent (WO\/2017\/106932) and are co-founders and shareholders of Mogrify Ltd., a cell therapy company. X.L., J.P.T. and J.M.P. are co-inventors on two provisional patent applications (application numbers: 2020904338 and 2020904340) filed by Monash University in relation to the generation of iBlastoids and derivation of stem cell lines from iBlastoids. The other authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}