{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T01:59:10Z","timestamp":1793066350954,"version":"4.5.0"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"7878","license":[{"start":{"date-parts":[[2021,9,15]],"date-time":"2021-09-15T00:00:00Z","timestamp":1631664000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,9,15]],"date-time":"2021-09-15T00:00:00Z","timestamp":1631664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2021,9,30]]},"DOI":"10.1038\/s41586-021-03899-0","type":"journal-article","created":{"date-parts":[[2021,9,15]],"date-time":"2021-09-15T12:03:19Z","timestamp":1631707399000},"page":"698-702","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":138,"title":["Rational design of a new antibiotic class for drug-resistant infections"],"prefix":"10.1038","volume":"597","author":[{"given":"Thomas F.","family":"Durand-Reville","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7110-0493","authenticated-orcid":false,"given":"Alita A.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"O\u2019Donnell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Sylvester","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satenig","family":"Guler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramkumar","family":"Iyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2662-6146","authenticated-orcid":false,"given":"Adam B.","family":"Shapiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole M.","family":"Carter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilo","family":"Velez-Vega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir H.","family":"Moussa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M.","family":"McLeod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Tanudra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janelle","family":"Comita-Prevoir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan A.","family":"Romero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoan","family":"Huynh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Ferguson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2218-9986","authenticated-orcid":false,"given":"Peter S.","family":"Horanyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Mayclin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry S.","family":"Heine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George L.","family":"Drusano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason E.","family":"Cummings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Slayden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1573-7422","authenticated-orcid":false,"given":"Ruben A.","family":"Tommasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,15]]},"reference":[{"key":"3899_CR1","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","volume":"18","author":"E Tacconelli","year":"2018","unstructured":"Tacconelli, E. et al. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect. Dis. 18, 318\u2013327 (2018).","journal-title":"Lancet Infect. Dis."},{"key":"3899_CR2","doi-asserted-by":"publisher","first-page":"a025239","DOI":"10.1101\/cshperspect.a025239","volume":"7","author":"RA Bonomo","year":"2016","unstructured":"Bonomo, R. A. Beta-lactamases: a focus on current challenges. Cold Spring Harb. Perspect. Med. 7, a025239 (2016).","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"3899_CR3","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1038\/s41579-019-0159-8","volume":"17","author":"K Bush","year":"2019","unstructured":"Bush, K. & Bradford, P. A. Interplay between \u03b2-lactamases and new \u03b2-lactamase inhibitors. Nat. Rev. Microbiol. 17, 295\u2013306 (2019).","journal-title":"Nat. Rev. Microbiol."},{"key":"3899_CR4","doi-asserted-by":"publisher","first-page":"11663","DOI":"10.1073\/pnas.1205073109","volume":"109","author":"DE Ehmann","year":"2012","unstructured":"Ehmann, D. E. et al. Avibactam is a covalent, reversible, non-\u03b2-lactam \u03b2-lactamase inhibitor. Proc. Natl Acad. Sci. USA 109, 11663\u201311668 (2012).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"3899_CR5","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1093\/jac\/dkw593","volume":"72","author":"DM Livermore","year":"2017","unstructured":"Livermore, D. M., Mushtaq, S., Warner, M., Vickers, A. & Woodford, N. In vitro activity of cefepime\/zidebactam (WCK 5222) against Gram-negative bacteria. J. Antimicrob. Chemother. 72, 1373\u20131385 (2017).","journal-title":"J. Antimicrob. Chemother."},{"key":"3899_CR6","doi-asserted-by":"publisher","first-page":"2779","DOI":"10.1093\/jac\/dkv166","volume":"70","author":"A Morinaka","year":"2015","unstructured":"Morinaka, A. et al. OP0595, a new diazabicyclooctane: mode of action as a serine \u03b2-lactamase inhibitor, antibiotic and \u03b2-lactam 'enhancer'. J. Antimicrob. Chemother. 70, 2779\u20132786 (2015).","journal-title":"J. Antimicrob. Chemother."},{"key":"3899_CR7","doi-asserted-by":"publisher","first-page":"17104","DOI":"10.1038\/nmicrobiol.2017.104","volume":"2","author":"TF Durand-Reville","year":"2017","unstructured":"Durand-Reville, T. F. et al. ETX2514 is a broad-spectrum \u03b2-lactamase inhibitor for the treatment of drug-resistant Gram-negative bacteria including Acinetobacter baumannii. Nat. Microbiol. 2, 17104 (2017).","journal-title":"Nat. Microbiol."},{"key":"3899_CR8","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.1021\/acsinfecdis.0c00020","volume":"6","author":"AA Miller","year":"2020","unstructured":"Miller, A. A. et al. In vitro characterization of ETX1317, a broad-spectrum \u03b2-lactamase inhibitor that restores and enhances \u03b2-lactam activity against multi-drug-resistant Enterobacterales, including carbapenem-resistant strains. ACS Infect. Dis. 6, 1389\u20131397 (2020).","journal-title":"ACS Infect. Dis."},{"key":"3899_CR9","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.4155\/fmc-2016-0078","volume":"8","author":"DY Wang","year":"2016","unstructured":"Wang, D. Y., Abboud, M. I., Markoulides, M. S., Brem, J. & Schofield, C. J. The road to avibactam: the first clinically useful non-\u03b2-lactam working somewhat like a \u03b2-lactam. Future Med. Chem. 8, 1063\u20131084 (2016).","journal-title":"Future Med. Chem."},{"key":"3899_CR10","doi-asserted-by":"publisher","first-page":"4742","DOI":"10.1021\/acs.jmedchem.9b00338","volume":"62","author":"N Levy","year":"2019","unstructured":"Levy, N. et al. Structural basis for E. coli penicillin binding protein (PBP) 2 inhibition, a platform for drug design. J. Med. Chem. 62, 4742\u20134754 (2019).","journal-title":"J. Med. Chem."},{"key":"3899_CR11","first-page":"149","volume":"75","author":"JM Kidd","year":"2020","unstructured":"Kidd, J. M., Abdelraouf, K. & Nicolau, D. P. Efficacy of human-simulated bronchopulmonary exposures of cefepime, zidebactam and the combination (WCK 5222) against MDR Pseudomonas aeruginosa in a neutropenic murine pneumonia model. J. Antimicrob. Chemother. 75, 149\u2013155 (2020).","journal-title":"J. Antimicrob. Chemother."},{"key":"3899_CR12","doi-asserted-by":"publisher","first-page":"1378","DOI":"10.1021\/acsinfecdis.0c00019","volume":"6","author":"J O'Donnell","year":"2020","unstructured":"O'Donnell, J. et al. Pharmacokinetic\/pharmacodynamic determination and preclinical pharmacokinetics of the \u03b2-lactamase inhibitor ETX1317 and its orally available prodrug ETX0282. ACS Infect. Dis. 6, 1378\u20131388 (2020).","journal-title":"ACS Infect. Dis."},{"key":"3899_CR13","doi-asserted-by":"publisher","first-page":"2810","DOI":"10.1093\/jac\/dkw230","volume":"71","author":"M Doumith","year":"2016","unstructured":"Doumith, M., Mushtaq, S., Livermore, D. M. & Woodford, N. New insights into the regulatory pathways associated with the activation of the stringent response in bacterial resistance to the PBP2-targeted antibiotics, mecillinam and OP0595\/RG6080. J. Antimicrob. Chemother. 71, 2810\u20132814 (2016).","journal-title":"J. Antimicrob. Chemother."},{"key":"3899_CR14","unstructured":"Levasseur, P., Pace, J., Lee Coleman, K. & Lowther, J. Novel Combinations of Antibacterial Nitrogenous Heterocyclic Compounds with Other Antibacterial Compounds, and Use Thereof as Drugs. Patent Cooperation Treaty WO\/2010\/041112 (2010)."},{"key":"3899_CR15","unstructured":"Aszodi, J., Lampilas, M., Musicki, B., Rowlands, D. A. & Colette, P. Heterocyclic Compounds, Method for Preparing Same and Use Thereof as Medicines, in Particular as Antibacterial Agents. Patent Cooperation Treaty WO\/2002\/100860 (2001)."},{"key":"3899_CR16","doi-asserted-by":"publisher","first-page":"16169","DOI":"10.1073\/pnas.1311066110","volume":"110","author":"P Nonejuie","year":"2013","unstructured":"Nonejuie, P., Burkart, M., Pogliano, K. & Pogliano, J. Bacterial cytological profiling rapidly identifies the cellular pathways targeted by antibacterial molecules. Proc. Natl Acad. Sci. USA 110, 16169\u201316174 (2013).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"3899_CR17","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1128\/AAC.45.1.105-116.2001","volume":"45","author":"O Lomovskaya","year":"2001","unstructured":"Lomovskaya, O. et al. Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy. Antimicrob. Agents Chemother. 45, 105\u2013116 (2001).","journal-title":"Antimicrob. Agents Chemother."},{"key":"3899_CR18","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1128\/AAC.25.6.701","volume":"25","author":"P Viljanen","year":"1984","unstructured":"Viljanen, P. & Vaara, M. Susceptibility of Gram-negative bacteria to polymyxin B nonapeptide. Antimicrob. Agents Chemother. 25, 701\u2013705 (1984).","journal-title":"Antimicrob. Agents Chemother."},{"key":"3899_CR19","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1021\/acsinfecdis.6b00197","volume":"3","author":"R Iyer","year":"2017","unstructured":"Iyer, R. et al. Whole-cell-based assay to evaluate structure permeation relationships for carbapenem passage through the Pseudomonas aeruginosa porin OprD. ACS Infect. Dis. 3, 310\u2013319 (2017).","journal-title":"ACS Infect. Dis."},{"key":"3899_CR20","doi-asserted-by":"publisher","first-page":"698","DOI":"10.1093\/femsre\/fux020","volume":"41","author":"S Chevalier","year":"2017","unstructured":"Chevalier, S. et al. Structure, function and regulation of Pseudomonas aeruginosa porins. FEMS Microbiol. Rev. 41, 698\u2013722 (2017).","journal-title":"FEMS Microbiol. Rev."},{"key":"3899_CR21","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1146\/annurev.micro.56.012302.160310","volume":"56","author":"RE Hancock","year":"2002","unstructured":"Hancock, R. E. & Brinkman, F. S. Function of Pseudomonas porins in uptake and efflux. Ann. Rev. Microbiol. 56, 17\u201338 (2002).","journal-title":"Ann. Rev. Microbiol."},{"key":"3899_CR22","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1128\/MMBR.67.4.593-656.2003","volume":"67","author":"H Nikaido","year":"2003","unstructured":"Nikaido, H. Molecular basis of bacterial outer membrane permeability revisited. Microbiol. Mol. Biol. Rev. 67, 593\u2013656 (2003).","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"3899_CR23","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1128\/AAC.03954-14","volume":"59","author":"VN Kos","year":"2015","unstructured":"Kos, V. N. et al. The resistome of Pseudomonas aeruginosa in relationship to phenotypic susceptibility. Antimicrob. Agents Chemother. 59, 427\u2013436 (2015).","journal-title":"Antimicrob. Agents Chemother."},{"key":"3899_CR24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031092","volume":"7","author":"A D\u00f6tsch","year":"2012","unstructured":"D\u00f6tsch, A. et al. The Pseudomonas aeruginosa transcriptome in planktonic cultures and static biofilms using RNA sequencing. PLoS ONE 7, e31092 (2012).","journal-title":"PLoS ONE"},{"key":"3899_CR25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004518","volume":"10","author":"KH Turner","year":"2014","unstructured":"Turner, K. H., Everett, J., Trivedi, U., Rumbaugh, K. P. & Whiteley, M. Requirements for Pseudomonas aeruginosa acute burn and chronic surgical wound infection. PLoS Genet. 10, e1004518 (2014).","journal-title":"PLoS Genet."},{"key":"3899_CR26","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1038\/nature22308","volume":"545","author":"MF Richter","year":"2017","unstructured":"Richter, M. F. et al. Predictive compound accumulation rules yield a broad-spectrum antibiotic. Nature 545, 299\u2013304 (2017).","journal-title":"Nature"},{"key":"3899_CR27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001242","volume":"10","author":"E Eren","year":"2012","unstructured":"Eren, E. et al. Substrate specificity within a family of outer membrane carboxylate channels. PLoS Biol. 10, e1001242 (2012).","journal-title":"PLoS Biol."},{"key":"3899_CR28","doi-asserted-by":"publisher","first-page":"5465","DOI":"10.1021\/acsnano.6b08613","volume":"11","author":"H Bajaj","year":"2017","unstructured":"Bajaj, H. et al. Bacterial outer membrane porins as electrostatic nanosieves: exploring transport rules of small polar molecules. ACS Nano 11, 5465\u20135473 (2017).","journal-title":"ACS Nano"},{"key":"3899_CR29","first-page":"1","volume":"S1","author":"JJS Cadwell","year":"2012","unstructured":"Cadwell, J. J. S. The hollow fiber infection model for antimicrobial pharmacodynamics and pharmacokinetics. Adv. Pharmacoepidem. Drug Safety S1, 1\u20135 (2012).","journal-title":"Adv. Pharmacoepidem. Drug Safety"},{"key":"3899_CR30","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/s10822-013-9644-8","volume":"27","author":"GM Sastry","year":"2013","unstructured":"Sastry, G. M., Adzhigirey, M., Day, T., Annabhimoju, R. & Sherman, W. Protein and ligand preparation: parameters, protocols, and influence on virtual screening enrichments. J. Comp. Aid. Mol. Des. 27, 221\u2013234 (2013).","journal-title":"J. Comp. Aid. Mol. Des."},{"key":"3899_CR31","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1021\/ct700301q","volume":"4","author":"B Hess","year":"2008","unstructured":"Hess, B., Kutzner, C., van der Spoel, D. & Lindahl, E. GROMACS 4:\u2009algorithms for highly efficient, load-balanced, and scalable molecular simulation. J. Chem. Theor. Comput. 4, 435\u2013447 (2008).","journal-title":"J. Chem. Theor. Comput."},{"key":"3899_CR32","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","volume":"14","author":"W Humphrey","year":"1996","unstructured":"Humphrey, W., Dalke, A. & Schulten, K. VMD: visual molecular dynamics. J. Mol. Graph. 14, 33\u201338 (1996).","journal-title":"J. Mol. Graph."},{"key":"3899_CR33","doi-asserted-by":"publisher","first-page":"1536","DOI":"10.1002\/jcc.22984","volume":"33","author":"DJ Sindhikara","year":"2012","unstructured":"Sindhikara, D. J., Yoshida, N. & Hirata, F. Placevent: an algorithm for prediction of explicit solvent atom distribution-application to HIV-1 protease and F-ATP synthase. J. Comp. Chem. 33, 1536\u20131543 (2012).","journal-title":"J. Comp. Chem."},{"key":"3899_CR34","doi-asserted-by":"publisher","first-page":"3344","DOI":"10.1021\/ci500571h","volume":"54","author":"C Velez-Vega","year":"2014","unstructured":"Velez-Vega, C., McKay, D. J., Aravamuthan, V., Pearlstein, R. & Duca, J. S. Time-averaged distributions of solute and solvent motions: exploring proton wires of GFP and PfM2DH. J. Chem. Inform. Model. 54, 3344\u20133361 (2014).","journal-title":"J. Chem. Inform. Model."},{"key":"3899_CR35","unstructured":"M07: Methods for Dilution Antimicrobial Susceptibilty Tests for Bacteria that Grow Aerobically 11th edn (Clinical and Laboratory Standards Institute, 2018)."},{"key":"3899_CR36","unstructured":"M26: Methods for Determining Bactericidal Activity 19th edn (Clinical and Laboratory Standards Institute, 1999)."},{"key":"3899_CR37","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1021\/acsinfecdis.7b00168","volume":"4","author":"R Iyer","year":"2018","unstructured":"Iyer, R., Moussa, S. H., Durand-R\u00e9ville, T. F., Tommasi, R. & Miller, A. Acinetobacter baumannii OmpA is a selective antibiotic permeant porin. ACS Infect. Dis. 4, 373\u2013381 (2018).","journal-title":"ACS Infect. Dis."},{"key":"3899_CR38","doi-asserted-by":"publisher","first-page":"1680","DOI":"10.1128\/AAC.04808-14","volume":"59","author":"WF Penwell","year":"2015","unstructured":"Penwell, W. F. et al. Molecular mechanisms of sulbactam antibacterial activity and resistance determinants in Acinetobacter baumannii. Antimicrob. Agents Chemother. 59, 1680\u20131689 (2015).","journal-title":"Antimicrob. Agents Chemother."},{"key":"3899_CR39","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.ab.2014.06.004","volume":"463","author":"AB Shapiro","year":"2014","unstructured":"Shapiro, A. B., Gao, N., Gu, R. F. & Thresher, J. Fluorescence anisotropy-based measurement of Pseudomonas aeruginosa penicillin-binding protein 2 transpeptidase inhibitor acylation rate constants. Anal. Biochem. 463, 15\u201322 (2014).","journal-title":"Anal. Biochem."},{"key":"3899_CR40","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.ab.2013.04.009","volume":"439","author":"AB Shapiro","year":"2013","unstructured":"Shapiro, A. B., Gu, R. F., Gao, N., Livchak, S. & Thresher, J. Continuous fluorescence anisotropy-based assay of BOCILLIN FL penicillin reaction with penicillin binding protein 3. Anal. Biochem. 439, 37\u201343 (2013).","journal-title":"Anal. Biochem."},{"key":"3899_CR41","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1021\/acsmedchemlett.5b00026","volume":"6","author":"KE Murphy-Benenato","year":"2015","unstructured":"Murphy-Benenato, K. E. et al. SAR and structural analysis of siderophore-conjugated monocarbam inhibitors of PBP3. ACS Med. Chem. Lett. 6, 537\u2013542 (2015).","journal-title":"ACS Med. Chem. Lett."},{"key":"3899_CR42","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"PD Adams","year":"2010","unstructured":"Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D 66, 213\u2013221 (2010).","journal-title":"Acta Crystallogr. D"},{"key":"3899_CR43","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1093\/infdis\/147.5.910","volume":"147","author":"AU Gerber","year":"1983","unstructured":"Gerber, A. U. et al. Impact of dosing intervals on activity of gentamicin and ticarcillin against Pseudomonas aeruginosa in granulocytopenic mice. J. Infect. Dis. 147, 910\u2013917 (1983).","journal-title":"J. Infect. Dis."},{"key":"3899_CR44","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1093\/jac\/18.Supplement_E.67","volume":"18","author":"S Gudmundsson","year":"1986","unstructured":"Gudmundsson, S., Vogelman, B. & Craig, W. A. The in-vivo postantibiotic effect of imipenem and other new antimicrobials. J. Antimicrob. Chemother. 18, 67\u201373 (1986).","journal-title":"J. Antimicrob. Chemother."},{"key":"3899_CR45","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1111\/j.1472-8206.2008.00633.x","volume":"22","author":"S Goutelle","year":"2008","unstructured":"Goutelle, S. et al. The Hill equation: a review of its capabilities in pharmacological modelling. Fundam. Clin. Pharmacol. 22, 633\u2013648 (2008).","journal-title":"Fundam. Clin. Pharmacol."},{"key":"3899_CR46","unstructured":"Institute of Laboratory Animal Resources Commission of Life Sciences. A Guide for the Care and Use of Laboratory Animals (National Academy Press, 1996)."},{"key":"3899_CR47","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1128\/AAC.42.3.675","volume":"42","author":"WR Byrne","year":"1998","unstructured":"Byrne, W. R. et al. Antibiotic treatment of experimental pneumonic plague in mice. Antimicrob. Agents Chemother. 42, 675\u2013681 (1998).","journal-title":"Antimicrob. Agents Chemother."},{"key":"3899_CR48","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/0021-8502(73)90006-2","volume":"4","author":"KR May","year":"1973","unstructured":"May, K. R. The Collison nebulizer: description, performance and application. J. Aerosol Sci. 4, 235\u2013243 (1973).","journal-title":"J. Aerosol Sci."},{"key":"3899_CR49","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.vascn.2003.07.001","volume":"49","author":"JM Hartings","year":"2004","unstructured":"Hartings, J. M. & Roy, C. J. The automated bioaerosol exposure system: preclinical platform development and a respiratory dosimetry application with nonhuman primates. J. Pharmacol. Toxicol. Methods 49, 39\u201355 (2004).","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"3899_CR50","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1152\/ajplegacy.1947.150.1.70","volume":"150","author":"AC Guyton","year":"1947","unstructured":"Guyton, A. C. Measurement of the respiratory volumes of laboratory animals. Am. J. Physiol. 150, 70\u201377 (1947).","journal-title":"Am. J. Physiol."},{"key":"3899_CR51","doi-asserted-by":"publisher","first-page":"58","DOI":"10.3201\/eid2101.131570","volume":"21","author":"J Morris","year":"2015","unstructured":"Morris, J. et al. Neurotropic threat characterization of Burkholderia pseudomallei strains. Emerg. Infect Dis. 21, 58\u201363 (2015).","journal-title":"Emerg. Infect Dis."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41586-021-03899-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41586-021-03899-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41586-021-03899-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T11:06:00Z","timestamp":1632913560000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41586-021-03899-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,15]]},"references-count":51,"journal-issue":{"issue":"7878","published-print":{"date-parts":[[2021,9,30]]}},"alternative-id":["3899"],"URL":"https:\/\/doi.org\/10.1038\/s41586-021-03899-0","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.740813882.793588978","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.740813882.793590464","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.740813882.793589244","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,15]]},"assertion":[{"value":"20 October 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 August 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 September 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"T.F.D.-R., A.A.M., J.P.O., X.W., M.A.S., S.G., R.I., A.B.S., N.M.C., C.V.-V., S.H.M., S.M.M., A.C., A.M.T., J.Z., J.C.-P., J.A.R. and R.A.T. are current or former employees of Entasis Therapeutics. H.H., A.D.F., P.S.H., S.J.M., H.S.H., G.L.D., J.E.C. and R.A.S. have no competing interests to declare.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}