{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T14:19:32Z","timestamp":1793283572453,"version":"4.5.0"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"8001","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2024,2,29]]},"DOI":"10.1038\/s41586-024-07055-2","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T17:02:40Z","timestamp":1707325360000},"page":"1141-1148","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Allosteric modulation and G-protein selectivity of the Ca2+-sensing receptor"],"prefix":"10.1038","volume":"626","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6286-8134","authenticated-orcid":false,"given":"Feng","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Guo","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8758-0954","authenticated-orcid":false,"given":"Yang","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2131-866X","authenticated-orcid":false,"given":"Sabrina N.","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5710-9419","authenticated-orcid":false,"given":"Magda","family":"Zaoralov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3238-3213","authenticated-orcid":false,"given":"Maka\u00eda M.","family":"Papasergi-Scott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8359-0653","authenticated-orcid":false,"given":"Ting-Jia","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2610-680X","authenticated-orcid":false,"given":"Michael J.","family":"Robertson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alpay B.","family":"Seven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0056-3869","authenticated-orcid":false,"given":"Lingjun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4251-4416","authenticated-orcid":false,"given":"Jesper M.","family":"Mathiesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0238-7846","authenticated-orcid":false,"given":"Georgios","family":"Skiniotis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"7055_CR1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.pharmthera.2011.01.006","volume":"130","author":"J Kniazeff","year":"2011","unstructured":"Kniazeff, J., Pr\u00e9zeau, L., Rondard, P., Pin, J.-P. & Goudet, C. Dimers and beyond: the functional puzzles of class C GPCRs. Pharmacol. Ther. 130, 9\u201325 (2011).","journal-title":"Pharmacol. Ther."},{"key":"7055_CR2","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1038\/nrm1154","volume":"4","author":"AM Hofer","year":"2003","unstructured":"Hofer, A. M. & Brown, E. M. Extracellular calcium sensing and signalling. Nat. Rev. Mol. Cell Biol. 4, 530\u2013538 (2003).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"7055_CR3","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1038\/s41574-018-0115-0","volume":"15","author":"FM Hannan","year":"2019","unstructured":"Hannan, F. M., Kallay, E., Chang, W., Brandi, M. L. & Thakker, R. V. The calcium-sensing receptor in physiology and in calcitropic and noncalcitropic diseases. Nat. Rev. Endocrinol. 15, 33\u201351 (2019).","journal-title":"Nat. Rev. Endocrinol."},{"key":"7055_CR4","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1186\/s13023-017-0570-z","volume":"12","author":"C Vahe","year":"2017","unstructured":"Vahe, C. et al. Diseases associated with calcium-sensing receptor. Orphanet J. Rare Dis. 12, 19 (2017).","journal-title":"Orphanet J. Rare Dis."},{"key":"7055_CR5","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1016\/j.semnephrol.2014.10.001","volume":"34","author":"ZA Massy","year":"2014","unstructured":"Massy, Z. A., Henaut, L., Larsson, T. E. & Vervloet, M. G. Calcium-sensing receptor activation in chronic kidney disease: effects beyond parathyroid hormone control. Semin. Nephrol. 34, 648\u2013659 (2014).","journal-title":"Semin. Nephrol."},{"key":"7055_CR6","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/S0140-6736(13)60439-0","volume":"382","author":"K-U Eckardt","year":"2013","unstructured":"Eckardt, K.-U. et al. Evolving importance of kidney disease: from subspecialty to global health burden. Lancet 382, 158\u2013169 (2013).","journal-title":"Lancet"},{"key":"7055_CR7","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1146\/annurev.pharmtox.011008.145533","volume":"50","author":"CM Niswender","year":"2010","unstructured":"Niswender, C. M. & Conn, P. J. Metabotropic glutamate receptors: physiology, pharmacology, and disease. Annu. Rev. Pharmacol. Toxicol. 50, 295\u2013322 (2010).","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"7055_CR8","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1124\/pr.119.019133","volume":"73","author":"KJ Gregory","year":"2021","unstructured":"Gregory, K. J. & Goudet, C. International union of basic and clinical pharmacology. CXI. Pharmacology, signaling, and physiology of metabotropic glutamate receptors. Pharmacol. Rev. 73, 521\u2013569 (2021).","journal-title":"Pharmacol. Rev."},{"key":"7055_CR9","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1124\/pr.119.018531","volume":"72","author":"K Leach","year":"2020","unstructured":"Leach, K. et al. International Union of Basic and Clinical Pharmacology. CVIII. Calcium-sensing receptor nomenclature, pharmacology, and function. Pharmacol. Rev. 72, 558\u2013604 (2020).","journal-title":"Pharmacol. Rev."},{"key":"7055_CR10","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1038\/s41586-021-03680-3","volume":"595","author":"AB Seven","year":"2021","unstructured":"Seven, A. B. et al. G-protein activation by a metabotropic glutamate receptor. Nature 595, 450\u2013454 (2021).","journal-title":"Nature"},{"key":"7055_CR11","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03495-2","volume":"594","author":"S Lin","year":"2021","unstructured":"Lin, S. et al. Structures of Gi-bound metabotropic glutamate receptors mGlu2 and mGlu4. Nature 594, 583\u2013588 (2021).","journal-title":"Nature"},{"key":"7055_CR12","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1038\/s41586-021-03507-1","volume":"594","author":"C Shen","year":"2021","unstructured":"Shen, C. et al. Structural basis of GABAB receptor\u2013Gi protein coupling. Nature 594, 594\u2013598 (2021).","journal-title":"Nature"},{"key":"7055_CR13","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1210\/er.2009-0043","volume":"32","author":"AL Magno","year":"2011","unstructured":"Magno, A. L., Ward, B. K. & Ratajczak, T. The calcium-sensing receptor: a molecular perspective. Endocr. Rev. 32, 3\u201330 (2011).","journal-title":"Endocr. Rev."},{"key":"7055_CR14","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12399-9","volume":"10","author":"PP Centeno","year":"2019","unstructured":"Centeno, P. P. et al. Phosphate acts directly on the calcium-sensing receptor to stimulate parathyroid hormone secretion. Nat. Commun. 10, 4693 (2019).","journal-title":"Nat. Commun."},{"key":"7055_CR15","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1038\/cr.2016.36","volume":"26","author":"K Leach","year":"2016","unstructured":"Leach, K. et al. Towards a structural understanding of allosteric drugs at the human calcium-sensing receptor. Cell Res. 26, 574\u2013592 (2016).","journal-title":"Cell Res."},{"key":"7055_CR16","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1038\/s41422-021-00474-0","volume":"31","author":"S Ling","year":"2021","unstructured":"Ling, S. et al. Structural mechanism of cooperative activation of the human calcium-sensing receptor by Ca2+ ions and l-tryptophan. Cell Res. 31, 383\u2013394 (2021).","journal-title":"Cell Res."},{"key":"7055_CR17","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1038\/s41586-021-03691-0","volume":"595","author":"Y Gao","year":"2021","unstructured":"Gao, Y. et al. Asymmetric activation of the calcium-sensing receptor homodimer. Nature 595, 455\u2013459 (2021).","journal-title":"Nature"},{"key":"7055_CR18","doi-asserted-by":"publisher","first-page":"e2115849118","DOI":"10.1073\/pnas.2115849118","volume":"118","author":"J Park","year":"2021","unstructured":"Park, J. et al. Symmetric activation and modulation of the human calcium-sensing receptor. Proc. Natl Acad. Sci. USA 118, e2115849118 (2021).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"7055_CR19","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1038\/s41589-020-0535-8","volume":"16","author":"RH Olsen","year":"2020","unstructured":"Olsen, R. H. et al. TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat. Chem. Biol. 16, 841\u2013849 (2020).","journal-title":"Nat. Chem. Biol."},{"key":"7055_CR20","doi-asserted-by":"publisher","first-page":"ra123","DOI":"10.1126\/scisignal.aab4068","volume":"8","author":"I Masuho","year":"2015","unstructured":"Masuho, I. et al. Distinct profiles of functional discrimination among G proteins determine the actions of G protein\u2013coupled receptors. Sci. Signal. 8, ra123 (2015).","journal-title":"Sci. Signal."},{"key":"7055_CR21","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1016\/0092-8674(95)90220-1","volume":"83","author":"MA Wall","year":"1995","unstructured":"Wall, M. A. et al. The structure of the G-protein heterotrimer Gi\u03b11\u03b21\u03b32. Cell 83, 1047\u20131058 (1995).","journal-title":"Cell"},{"key":"7055_CR22","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17933-8","volume":"11","author":"J Duan","year":"2020","unstructured":"Duan, J. et al. Cryo-EM structure of an activated VIP1 receptor-G protein complex revealed by a NanoBiT tethering strategy. Nat. Commun. 11, 4121 (2020).","journal-title":"Nat. Commun."},{"key":"7055_CR23","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1111\/j.1365-2796.2006.01684.x","volume":"260","author":"H Timmers","year":"2006","unstructured":"Timmers, H., Karperien, M., Hamdy, N., De Boer, H. & Hermus, A. Normalization of serum calcium by cinacalcet in a patient with hypercalcaemia due to a de novo inactivating mutation of the calcium-sensing receptor. J. Intern. Med. 260, 177\u2013182 (2006).","journal-title":"J. Intern. Med."},{"key":"7055_CR24","doi-asserted-by":"publisher","first-page":"2768","DOI":"10.1093\/hmg\/dds105","volume":"21","author":"FM Hannan","year":"2012","unstructured":"Hannan, F. M. et al. Identification of 70 calcium-sensing receptor mutations in hyper-and hypo-calcaemic patients: evidence for clustering of extracellular domain mutations at calcium-binding sites. Hum. Mol. Genet. 21, 2768\u20132778 (2012).","journal-title":"Hum. Mol. Genet."},{"key":"7055_CR25","doi-asserted-by":"publisher","first-page":"2681","DOI":"10.1210\/jcem.87.6.8531","volume":"87","author":"T Nagase","year":"2002","unstructured":"Nagase, T. et al. A family of autosomal dominant hypocalcemia with a positive correlation between serum calcium and magnesium: identification of a novel gain of function mutation (Ser820Phe) in the calcium-sensing receptor. J. Clin. Endocrinol. Metab. 87, 2681\u20132687 (2002).","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"7055_CR26","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1007\/s00431-003-1331-7","volume":"163","author":"M Shiohara","year":"2004","unstructured":"Shiohara, M. et al. A novel gain-of-function mutation (F821L) in the transmembrane domain of calcium-sensing receptor is a cause of severe sporadic hypoparathyroidism. Eur. J. Pediatr. 163, 94\u201398 (2004).","journal-title":"Eur. J. Pediatr."},{"key":"7055_CR27","doi-asserted-by":"publisher","first-page":"5113","DOI":"10.1074\/jbc.M413403200","volume":"280","author":"J Hu","year":"2005","unstructured":"Hu, J. et al. A region in the seven-transmembrane domain of the human Ca2+ receptor critical for response to Ca2+. J. Biol. Chem. 280, 5113\u20135120 (2005).","journal-title":"J. Biol. Chem."},{"key":"7055_CR28","doi-asserted-by":"publisher","first-page":"C1315","DOI":"10.1152\/ajpcell.1997.273.4.C1315","volume":"273","author":"SJ Quinn","year":"1997","unstructured":"Quinn, S. J. et al. The Ca2+-sensing receptor: a target for polyamines. Am. J. Physiol. 273, C1315\u2013C1323 (1997).","journal-title":"Am. J. Physiol."},{"key":"7055_CR29","doi-asserted-by":"publisher","first-page":"14896","DOI":"10.1074\/jbc.R116.734780","volume":"291","author":"AJ Michael","year":"2016","unstructured":"Michael, A. J. Polyamines in eukaryotes, bacteria, and archaea. J. Biol. Chem. 291, 14896\u201314903 (2016).","journal-title":"J. Biol. Chem."},{"key":"7055_CR30","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29897-y","volume":"13","author":"MR Schamber","year":"2022","unstructured":"Schamber, M. R. & Vafabakhsh, R. Mechanism of sensitivity modulation in the calcium-sensing receptor via electrostatic tuning. Nat. Commun. 13, 2194 (2022).","journal-title":"Nat. Commun."},{"key":"7055_CR31","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1677\/JME-08-0164","volume":"42","author":"DE Cole","year":"2009","unstructured":"Cole, D. E. et al. Calcium-sensing receptor mutations and denaturing high performance liquid chromatography. J. Mol. Endocrinol. 42, 331\u2013339 (2009).","journal-title":"J. Mol. Endocrinol."},{"key":"7055_CR32","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1210\/jc.2002-020081","volume":"88","author":"Y Tan","year":"2003","unstructured":"Tan, Y. et al. Autosomal dominant hypocalcemia: a novel activating mutation (E604K) in the cysteine-rich domain of the calcium-sensing receptor. J. Clin. Endocrinol. Metab. 88, 605\u2013610 (2003).","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"7055_CR33","first-page":"18-0107","volume":"2018","author":"B Kwan","year":"2018","unstructured":"Kwan, B. et al. A novel CASR mutation (p. Glu757Lys) causing autosomal dominant hypocalcaemia type 1. Endocrinol. Diabetes Metab. Case Rep. 2018, 18-0107 (2018).","journal-title":"Endocrinol. Diabetes Metab. Case Rep."},{"key":"7055_CR34","doi-asserted-by":"publisher","first-page":"4814","DOI":"10.1073\/pnas.97.9.4814","volume":"97","author":"AD Conigrave","year":"2000","unstructured":"Conigrave, A. D., Quinn, S. J. & Brown, E. M. l-amino acid sensing by the extracellular Ca2+-sensing receptor. Proc. Natl Acad. Sci. USA 97, 4814\u20134819 (2000).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"7055_CR35","doi-asserted-by":"publisher","first-page":"884","DOI":"10.3390\/toxins3070884","volume":"3","author":"S Mangmool","year":"2011","unstructured":"Mangmool, S. & Kurose, H. Gi\/o protein-dependent and-independent actions of pertussis toxin (PTX). Toxins 3, 884\u2013899 (2011).","journal-title":"Toxins"},{"key":"7055_CR36","doi-asserted-by":"publisher","first-page":"2185","DOI":"10.1210\/jc.2015-3442","volume":"101","author":"R Vargas-Poussou","year":"2016","unstructured":"Vargas-Poussou, R. et al. Familial hypocalciuric hypercalcemia types 1 and 3 and primary hyperparathyroidism: similarities and differences. J. Clin. Endocrinol. Metab. 101, 2185\u20132195 (2016).","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"7055_CR37","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.jpeds.2011.01.035","volume":"159","author":"D Koltin","year":"2011","unstructured":"Koltin, D. et al. Mild infantile hypercalcemia: diagnostic tests and outcomes. J. Pediatr. 159, 215\u2013221 (2011).","journal-title":"J. Pediatr."},{"key":"7055_CR38","doi-asserted-by":"publisher","first-page":"31355","DOI":"10.1074\/jbc.272.50.31355","volume":"272","author":"K Ray","year":"1997","unstructured":"Ray, K., Fan, G.-F., Goldsmith, P. K. & Spiegel, A. M. The carboxyl terminus of the human calcium receptor: requirements for cell-surface expression and signal transduction. J. Biol. Chem. 272, 31355\u201331361 (1997).","journal-title":"J. Biol. Chem."},{"key":"7055_CR39","doi-asserted-by":"publisher","first-page":"2476","DOI":"10.1056\/NEJMoa1300253","volume":"368","author":"MA Nesbit","year":"2013","unstructured":"Nesbit, M. A. et al. Mutations affecting G-protein subunit \u03b111 in hypercalcemia and hypocalcemia. N. Engl. J. Med. 368, 2476\u20132486 (2013).","journal-title":"N. Engl. J. Med."},{"key":"7055_CR40","doi-asserted-by":"publisher","first-page":"21267","DOI":"10.1074\/jbc.273.33.21267","volume":"273","author":"M Bai","year":"1998","unstructured":"Bai, M. et al. Protein kinase C phosphorylation of threonine at position 888 in Ca2+o-sensing receptor (CaR) inhibits coupling to Ca2+ store release. J. Biol. Chem. 273, 21267\u201321275 (1998).","journal-title":"J. Biol. Chem."},{"key":"7055_CR41","doi-asserted-by":"publisher","first-page":"15048","DOI":"10.1074\/jbc.M607469200","volume":"282","author":"SL Davies","year":"2007","unstructured":"Davies, S. L., Ozawa, A., McCormick, W. D., Dvorak, M. M. & Ward, D. T. Protein kinase C-mediated phosphorylation of the calcium-sensing receptor is stimulated by receptor activation and attenuated by calyculin-sensitive phosphatase activity. J. Biol. Chem. 282, 15048\u201315056 (2007).","journal-title":"J. Biol. Chem."},{"key":"7055_CR42","doi-asserted-by":"publisher","first-page":"50543","DOI":"10.1074\/jbc.M205798200","volume":"277","author":"YF Jiang","year":"2002","unstructured":"Jiang, Y. F. et al. Protein kinase C (PKC) phosphorylation of the Ca2+o-sensing receptor (CaR) modulates functional interaction of G proteins with the CaR cytoplasmic tail. J. Biol. Chem. 277, 50543\u201350549 (2002).","journal-title":"J. Biol. Chem."},{"key":"7055_CR43","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1530\/EJE-10-0907","volume":"164","author":"S Lazarus","year":"2011","unstructured":"Lazarus, S. et al. A novel mutation of the primary protein kinase C phosphorylation site in the calcium-sensing receptor causes autosomal dominant hypocalcemia. Eur. J. Endocrinol. 164, 429\u2013435 (2011).","journal-title":"Eur. J. Endocrinol."},{"key":"7055_CR44","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1038\/366575a0","volume":"366","author":"EM Brown","year":"1993","unstructured":"Brown, E. M. et al. Cloning and characterization of an extracellular Ca2+-sensing receptor from bovine parathyroid. Nature 366, 575\u2013580 (1993).","journal-title":"Nature"},{"key":"7055_CR45","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1038\/s41594-022-00727-5","volume":"29","author":"MJ Robertson","year":"2022","unstructured":"Robertson, M. J., Meyerowitz, J. G., Panova, O., Borrelli, K. & Skiniotis, G. Plasticity in ligand recognition at somatostatin receptors. Nat. Struct. Mol. Biol. 29, 210\u2013217 (2022).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"7055_CR46","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06002-w","volume":"9","author":"S Maeda","year":"2018","unstructured":"Maeda, S. et al. Development of an antibody fragment that stabilizes GPCR\/G-protein complexes. Nat. Commun. 9, 3712 (2018).","journal-title":"Nat. Commun."},{"key":"7055_CR47","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1021\/nl025623k","volume":"2","author":"TH Bayburt","year":"2002","unstructured":"Bayburt, T. H., Grinkova, Y. V. & Sligar, S. G. Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins. Nano Lett. 2, 853\u2013856 (2002).","journal-title":"Nano Lett."},{"key":"7055_CR48","doi-asserted-by":"crossref","unstructured":"Peisley, A. & Skiniotis, G. 2D projection analysis of GPCR complexes by negative stain electron microscopy. Methods Mol. Biol. 1335, 29\u201338 (2015).","DOI":"10.1007\/978-1-4939-2914-6_3"},{"key":"7055_CR49","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.jsb.2005.07.007","volume":"152","author":"DN Mastronarde","year":"2005","unstructured":"Mastronarde, D. N. Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36\u201351 (2005).","journal-title":"J. Struct. Biol."},{"key":"7055_CR50","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/nmeth.4169","volume":"14","author":"A Punjani","year":"2017","unstructured":"Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290\u2013296 (2017).","journal-title":"Nat. Methods"},{"key":"7055_CR51","doi-asserted-by":"publisher","first-page":"e42166","DOI":"10.7554\/eLife.42166","volume":"7","author":"J Zivanov","year":"2018","unstructured":"Zivanov, J. et al. New tools for automated high-resolution cryo-EM structure determination in RELION-3. eLife 7, e42166 (2018).","journal-title":"eLife"},{"key":"7055_CR52","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"EF Pettersen","year":"2004","unstructured":"Pettersen, E. F. et al. UCSF Chimera\u2014a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605\u20131612 (2004).","journal-title":"J. Comput. Chem."},{"key":"7055_CR53","doi-asserted-by":"publisher","first-page":"1230","DOI":"10.1038\/s41589-021-00866-8","volume":"17","author":"X Zhang","year":"2021","unstructured":"Zhang, X. et al. Structures of the human cholecystokinin receptors bound to agonists and antagonists. Nat. Chem. Biol. 17, 1230\u20131237 (2021).","journal-title":"Nat. Chem. Biol."},{"key":"7055_CR54","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"P Emsley","year":"2010","unstructured":"Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D 66, 486\u2013501 (2010).","journal-title":"Acta Crystallogr. D"},{"key":"7055_CR55","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1107\/S2059798319011471","volume":"75","author":"D Liebschner","year":"2019","unstructured":"Liebschner, D. et al. Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix. Acta Crystallogr. D 75, 861\u2013877 (2019).","journal-title":"Acta Crystallogr. D"},{"key":"7055_CR56","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1107\/S0907444909042073","volume":"66","author":"VB Chen","year":"2010","unstructured":"Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr. D 66, 12\u201321 (2010).","journal-title":"Acta Crystallogr. D"},{"key":"7055_CR57","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1002\/pro.3943","volume":"30","author":"EF Pettersen","year":"2021","unstructured":"Pettersen, E. F. et al. UCSF ChimeraX: structure visualization for researchers, educators, and developers. Protein Sci. 30, 70\u201382 (2021).","journal-title":"Protein Sci."},{"key":"7055_CR58","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1111\/bcpt.12778","volume":"121","author":"CB Falk\u2010Petersen","year":"2017","unstructured":"Falk\u2010Petersen, C. B. et al. Development of a robust mammalian cell\u2010based assay for studying recombinant \u03b14\u03b21\/3\u03b4 GABAA receptor subtypes. Basic Clin. Pharmacol. Toxicol. 121, 119\u2013129 (2017).","journal-title":"Basic Clin. Pharmacol. Toxicol."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41586-024-07055-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41586-024-07055-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41586-024-07055-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T04:42:11Z","timestamp":1709181731000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41586-024-07055-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,7]]},"references-count":58,"journal-issue":{"issue":"8001","published-print":{"date-parts":[[2024,2,29]]}},"alternative-id":["7055"],"URL":"https:\/\/doi.org\/10.1038\/s41586-024-07055-2","relation":{},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,7]]},"assertion":[{"value":"2 June 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 January 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 February 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"G.S. is a co-founder of and consultant for Deep Apple Therapeutics. The remaining authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}