{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T01:59:23Z","timestamp":1793066363706,"version":"4.5.0"},"reference-count":57,"publisher":"Springer Science and Business Media LLC","issue":"8035","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2024,10,24]]},"DOI":"10.1038\/s41586-024-07929-5","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T12:03:07Z","timestamp":1726056187000},"page":"936-943","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":123,"title":["A brain-to-gut signal controls intestinal fat absorption"],"prefix":"10.1038","volume":"634","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5070-2920","authenticated-orcid":false,"given":"Qianqian","family":"Lyu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3453-1726","authenticated-orcid":false,"given":"Wenzhi","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5526-6717","authenticated-orcid":false,"given":"Ruixin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinyun","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikram Babu","family":"Kasaragod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanru","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2657-6676","authenticated-orcid":false,"given":"Mingyang","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5167-3262","authenticated-orcid":false,"given":"Yuying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8556-8049","authenticated-orcid":false,"given":"Bing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aifang","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongrong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2860-3239","authenticated-orcid":false,"given":"Po","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weifang","family":"Rong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxi","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufang","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-1370","authenticated-orcid":false,"given":"Chunlei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fajun","family":"Nan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihe","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3443-9815","authenticated-orcid":false,"given":"Xiuna","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9383-7656","authenticated-orcid":false,"given":"Jiqiu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6027-3084","authenticated-orcid":false,"given":"Weiqing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,11]]},"reference":[{"key":"7929_CR1","unstructured":"Barrett, K. E., Barman, S. M., Brooks, H. L. and Yuan, J. Ganong\u2019s Review of Medical Physiology 26th edn. 475\u2013476 (McGraw-Hill Medical, 2019)."},{"key":"7929_CR2","unstructured":"Hall, J. E. Guyton and Hall Textbook of Medical Physiology 13th edn., 835\u2013841 (Elsevier, 2016)."},{"key":"7929_CR3","unstructured":"Koeppen, B. M. & Stanton, B. A. Berne & Levy Physiology 7th edn., 551\u2013553 (Elsevier, 2018)."},{"key":"7929_CR4","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1016\/S0140-6736(05)67402-8","volume":"366","author":"KGMM Alberti","year":"2005","unstructured":"Alberti, K. G. M. M., Zimmet, P. & Shaw, J. The metabolic syndrome\u2014a new worldwide definition. Lancet 366, 1059\u20131062 (2005).","journal-title":"Lancet"},{"key":"7929_CR5","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.pneurobio.2011.07.010","volume":"95","author":"FH Mourad","year":"2011","unstructured":"Mourad, F. H. & Saade, N. E. Neural regulation of intestinal nutrient absorption. Prog. Neurobiol. 95, 149\u2013162 (2011).","journal-title":"Prog. Neurobiol."},{"key":"7929_CR6","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1097\/MOL.0000000000000084","volume":"25","author":"MM Hussain","year":"2014","unstructured":"Hussain, M. M. Intestinal lipid absorption and lipoprotein formation. Curr. Opin. Lipidol. 25, 200\u2013206 (2014).","journal-title":"Curr. Opin. Lipidol."},{"key":"7929_CR7","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1038\/nrgastro.2016.5","volume":"13","author":"D Delacour","year":"2016","unstructured":"Delacour, D., Salomon, J., Robine, S. & Louvard, D. Plasticity of the brush border\u2014the yin and yang of intestinal homeostasis. Nat. Rev. Gastroenterol. Hepatol. 13, 161\u2013174 (2016).","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"7929_CR8","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1002\/jmor.1051660306","volume":"166","author":"DJ Chivers","year":"1980","unstructured":"Chivers, D. J. & Hladik, C. M. Morphology of the gastrointestinal tract in primates: comparisons with other mammals in relation to diet. J. Morphol. 166, 337\u2013386 (1980).","journal-title":"J. Morphol."},{"key":"7929_CR9","doi-asserted-by":"publisher","first-page":"1929","DOI":"10.1194\/jlr.M005744","volume":"51","author":"J Iqbal","year":"2010","unstructured":"Iqbal, J. et al. An intrinsic gut leptin\u2013melanocortin pathway modulates intestinal microsomal triglyceride transfer protein and lipid absorption. J. Lipid Res. 51, 1929\u20131942 (2010).","journal-title":"J. Lipid Res."},{"key":"7929_CR10","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1038\/nrgastro.2016.76","volume":"13","author":"RA Travagli","year":"2016","unstructured":"Travagli, R. A. & Anselmi, L. Vagal neurocircuitry and its influence on gastric motility. Nat. Rev. Gastroenterol. Hepatol. 13, 389\u2013401 (2016).","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"7929_CR11","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1002\/cphy.c130055","volume":"4","author":"KN Browning","year":"2014","unstructured":"Browning, K. N. & Travagli, R. A. Central nervous system control of gastrointestinal motility and secretion and modulation of gastrointestinal functions. Compr. Physiol. 4, 1339\u20131368 (2014).","journal-title":"Compr. Physiol."},{"key":"7929_CR12","doi-asserted-by":"publisher","first-page":"908","DOI":"10.3390\/nu13030908","volume":"13","author":"KN Browning","year":"2021","unstructured":"Browning, K. N. & Carson, K. E. Central neurocircuits regulating food intake in response to gut inputs\u2014preclinical evidence. Nutrients 13, 908 (2021).","journal-title":"Nutrients"},{"key":"7929_CR13","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/0016-5085(88)90608-7","volume":"94","author":"JE Doty","year":"1988","unstructured":"Doty, J. E. & Meyer, J. H. Vagotomy and antrectomy impairs canine fat absorption from solid but not liquid dietary sources. Gastroenterology 94, 50\u201356 (1988).","journal-title":"Gastroenterology"},{"key":"7929_CR14","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1002\/jcph.96","volume":"53","author":"Z Zhang","year":"2013","unstructured":"Zhang, Z., Lam, T. N. & Zuo, Z. Radix Puerariae: an overview of its chemistry, pharmacology, pharmacokinetics, and clinical use. J. Clin. Pharmacol. 53, 787\u2013811 (2013).","journal-title":"J. Clin. Pharmacol."},{"key":"7929_CR15","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1146\/annurev-pharmtox-010814-124803","volume":"55","author":"DJ Urban","year":"2015","unstructured":"Urban, D. J. & Roth, B. L. DREADDs (designer receptors exclusively activated by designer drugs): chemogenetic tools with therapeutic utility. Annu. Rev. Pharmacol. Toxicol. 55, 399\u2013417 (2015).","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"7929_CR16","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/mp.2016.218","volume":"23","author":"CM Mazzone","year":"2018","unstructured":"Mazzone, C. M. et al. Acute engagement of Gq-mediated signaling in the bed nucleus of the stria terminalis induces anxiety-like behavior. Mol. Psychiatry 23, 143\u2013153 (2018).","journal-title":"Mol. Psychiatry"},{"key":"7929_CR17","doi-asserted-by":"publisher","first-page":"1603","DOI":"10.1038\/ijo.2013.48","volume":"37","author":"L Gautron","year":"2013","unstructured":"Gautron, L., Zechner, J. F. & Aguirre, V. Vagal innervation patterns following Roux-en-Y gastric bypass in the mouse. Int. J. Obes. 37, 1603\u20131607 (2013).","journal-title":"Int. J. Obes."},{"key":"7929_CR18","doi-asserted-by":"publisher","first-page":"1521","DOI":"10.1172\/JCI103549","volume":"36","author":"B Borgstrom","year":"1957","unstructured":"Borgstrom, B., Dahlqvist, A., Lundh, G. & Sjovall, J. Studies of intestinal digestion and absorption in the human. J. Clin. Invest. 36, 1521\u20131536 (1957).","journal-title":"J. Clin. Invest."},{"key":"7929_CR19","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1136\/gut.2.1.23","volume":"2","author":"CC Booth","year":"1961","unstructured":"Booth, C. C., Read, A. E. & Jones, E. Studies on the site of fat absorption: 1. The sites of absorption of increasing doses of I-labelled triolein in the rat. Gut 2, 23\u201331 (1961).","journal-title":"Gut"},{"key":"7929_CR20","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1038\/s42255-021-00363-1","volume":"3","author":"MQ Ludwig","year":"2021","unstructured":"Ludwig, M. Q. et al. A genetic map of the mouse dorsal vagal complex and its role in obesity. Nat. Metab. 3, 530\u2013545 (2021).","journal-title":"Nat. Metab."},{"key":"7929_CR21","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.cmet.2011.01.010","volume":"13","author":"J Rossi","year":"2011","unstructured":"Rossi, J. et al. Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab. 13, 195\u2013204 (2011).","journal-title":"Cell Metab."},{"key":"7929_CR22","doi-asserted-by":"publisher","first-page":"1752","DOI":"10.1016\/j.clinthera.2018.08.014","volume":"40","author":"M Yang","year":"2018","unstructured":"Yang, M. et al. The effect of puerarin on carotid intima-media thickness in patients with active rheumatoid arthritis: a randomized controlled trial. Clin. Ther. 40, 1752\u20131764.e1 (2018).","journal-title":"Clin. Ther."},{"key":"7929_CR23","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1146\/annurev-biochem-060713-035708","volume":"83","author":"MJ Niphakis","year":"2014","unstructured":"Niphakis, M. J. & Cravatt, B. F. Enzyme inhibitor discovery by activity-based protein profiling. Annu. Rev. Biochem. 83, 341\u2013377 (2014).","journal-title":"Annu. Rev. Biochem."},{"key":"7929_CR24","doi-asserted-by":"publisher","first-page":"5456","DOI":"10.1073\/pnas.1322727111","volume":"111","author":"P Shieh","year":"2014","unstructured":"Shieh, P., Siegrist, M. S., Cullen, A. J. & Bertozzi, C. R. Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes. Proc. Natl Acad. Sci. USA 111, 5456\u20135461 (2014).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"7929_CR25","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1002\/cne.903590111","volume":"359","author":"JM Fritschy","year":"1995","unstructured":"Fritschy, J. M. & Mohler, H. GABAA-receptor heterogeneity in the adult rat brain: differential regional and cellular distribution of seven major subunits. J. Comp. Neurol. 359, 154\u2013194 (1995).","journal-title":"J. Comp. Neurol."},{"key":"7929_CR26","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1046\/j.1365-2982.1998.00110.x","volume":"10","author":"DV Sivarao","year":"1998","unstructured":"Sivarao, D. V., Krowicki, Z. K. & Hornby, P. J. Role of GABAA receptors in rat hindbrain nuclei controlling gastric motor function. Neurogastroenterol. Motil. 10, 305\u2013313 (1998).","journal-title":"Neurogastroenterol. Motil."},{"key":"7929_CR27","doi-asserted-by":"publisher","first-page":"40224","DOI":"10.1074\/jbc.R112.386664","volume":"287","author":"E Sigel","year":"2012","unstructured":"Sigel, E. & Steinmann, M. E. Structure, function, and modulation of GABAA receptors. J. Biol. Chem. 287, 40224\u201340231 (2012).","journal-title":"J. Biol. Chem."},{"key":"7929_CR28","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1038\/s41586-018-0833-4","volume":"565","author":"D Laverty","year":"2019","unstructured":"Laverty, D. et al. Cryo-EM structure of the human \u03b11\u03b23\u03b32 GABAA receptor in a lipid bilayer. Nature 565, 516\u2013520 (2019).","journal-title":"Nature"},{"key":"7929_CR29","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1038\/s41586-018-0832-5","volume":"565","author":"S Masiulis","year":"2019","unstructured":"Masiulis, S. et al. GABAA receptor signalling mechanisms revealed by structural pharmacology. Nature 565, 454\u2013459 (2019).","journal-title":"Nature"},{"key":"7929_CR30","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32212-4","volume":"13","author":"S Zhu","year":"2022","unstructured":"Zhu, S. et al. Structural and dynamic mechanisms of GABAA receptor modulators with opposing activities. Nat. Commun. 13, 4582 (2022).","journal-title":"Nat. Commun."},{"key":"7929_CR31","doi-asserted-by":"publisher","first-page":"1936","DOI":"10.1038\/s41594-023-01133-1","volume":"30","author":"VB Kasaragod","year":"2023","unstructured":"Kasaragod, V. B. et al. The molecular basis of drug selectivity for \u03b15 subunit-containing GABAA receptors. Nat. Struct. Mol. Biol. 30, 1936\u20131946 (2023).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"7929_CR32","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1111\/j.1469-7793.1999.0521t.x","volume":"517","author":"KN Browning","year":"1999","unstructured":"Browning, K. N., Renehan, W. E. & Travagli, R. A. Electrophysiological and morphological heterogeneity of rat dorsal vagal neurones which project to specific areas of the gastrointestinal tract. J. Physiol. 517, 521\u2013532 (1999).","journal-title":"J. Physiol."},{"key":"7929_CR33","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s00429-016-1241-x","volume":"222","author":"S Libbrecht","year":"2017","unstructured":"Libbrecht, S., Van den Haute, C., Malinouskaya, L., Gijsbers, R. & Baekelandt, V. Evaluation of WGA-Cre-dependent topological transgene expression in the rodent brain. Brain Struct. Funct. 222, 717\u2013733 (2017).","journal-title":"Brain Struct. Funct."},{"key":"7929_CR34","unstructured":"Bernard, C. M\u00e9moire sur le Pancr\u00e9as et sur le Role du Suc Pancr\u00e9atique dans les Ph\u00e9nom\u00e8nes Digestifs, Particuli\u00e8rement dans la Digestion des Mati\u00e8res Grasses Neutres (J.-B. Bailli\u00e8re, 1856)."},{"key":"7929_CR35","doi-asserted-by":"publisher","first-page":"2106","DOI":"10.1016\/j.neuron.2021.05.004","volume":"109","author":"J Tao","year":"2021","unstructured":"Tao, J. et al. Highly selective brain-to-gut communication via genetically defined vagus neurons. Neuron 109, 2106\u20132115.e4 (2021).","journal-title":"Neuron"},{"key":"7929_CR36","doi-asserted-by":"publisher","first-page":"51722","DOI":"10.1074\/jbc.M408351200","volume":"279","author":"DD Tang","year":"2004","unstructured":"Tang, D. D. & Gunst, S. J. The small GTPase Cdc42 regulates actin polymerization and tension development during contractile stimulation of smooth muscle. J. Biol. Chem. 279, 51722\u201351728 (2004).","journal-title":"J. Biol. Chem."},{"key":"7929_CR37","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1242\/jcs.105.4.1025","volume":"105","author":"M Berryman","year":"1993","unstructured":"Berryman, M., Franck, Z. & Bretscher, A. Ezrin is concentrated in the apical microvilli of a wide variety of epithelial cells whereas moesin is found primarily in endothelial cells. J. Cell Sci. 105, 1025\u20131043 (1993).","journal-title":"J. Cell Sci."},{"key":"7929_CR38","first-page":"G531","volume":"260","author":"RA Travagli","year":"1991","unstructured":"Travagli, R. A., Gillis, R. A., Rossiter, C. D. & Vicini, S. Glutamate and GABA-mediated synaptic currents in neurons of the rat dorsal motor nucleus of the vagus. Am. J. Physiol. 260, G531\u2013G536 (1991).","journal-title":"Am. J. Physiol."},{"key":"7929_CR39","first-page":"S12","volume":"21","author":"R Guerciolini","year":"1997","unstructured":"Guerciolini, R. Mode of action of orlistat. Int. J. Obes. Relat. Metab. Disord. 21, S12\u2013S23 (1997).","journal-title":"Int. J. Obes. Relat. Metab. Disord."},{"key":"7929_CR40","doi-asserted-by":"publisher","first-page":"f1936","DOI":"10.1136\/bmj.f1936","volume":"346","author":"IJ Douglas","year":"2013","unstructured":"Douglas, I. J., Langham, J., Bhaskaran, K., Brauer, R. & Smeeth, L. Orlistat and the risk of acute liver injury: self controlled case series study in UK Clinical Practice Research Datalink. BMJ 346, f1936 (2013).","journal-title":"BMJ"},{"key":"7929_CR41","doi-asserted-by":"publisher","first-page":"941","DOI":"10.1053\/j.gastro.2005.06.018","volume":"129","author":"R Capasso","year":"2005","unstructured":"Capasso, R. et al. Fatty acid amide hydrolase controls mouse intestinal motility in vivo. Gastroenterology 129, 941\u2013951 (2005).","journal-title":"Gastroenterology"},{"key":"7929_CR42","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1016\/j.cell.2016.02.010","volume":"164","author":"C Toda","year":"2016","unstructured":"Toda, C. et al. UCP2 regulates mitochondrial fission and ventromedial nucleus control of glucose responsiveness. Cell 164, 872\u2013883 (2016).","journal-title":"Cell"},{"key":"7929_CR43","doi-asserted-by":"publisher","first-page":"11245","DOI":"10.1021\/acs.joc.8b01831","volume":"83","author":"P Jackson","year":"2018","unstructured":"Jackson, P. & Lapinsky, D. J. Appendage and scaffold diverse fully functionalized small-molecule probes via a minimalist terminal alkyne-aliphatic diazirine isocyanide. J. Org. Chem. 83, 11245\u201311253 (2018).","journal-title":"J. Org. Chem."},{"key":"7929_CR44","doi-asserted-by":"publisher","first-page":"4259","DOI":"10.1021\/jacs.7b11639","volume":"140","author":"J Gao","year":"2018","unstructured":"Gao, J., Mfuh, A., Amako, Y. & Woo, C. M. Small molecule interactome mapping by photoaffinity labeling reveals binding site hotspots for the NSAIDs. J. Am. Chem. Soc. 140, 4259\u20134268 (2018).","journal-title":"J. Am. Chem. Soc."},{"key":"7929_CR45","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1002\/pro.2401","volume":"23","author":"Z Dostalova","year":"2014","unstructured":"Dostalova, Z. et al. Human \u03b11\u03b23\u03b32L \u03b3-aminobutyric acid type A receptors: high-level production and purification in a functional state. Protein Sci. 23, 157\u2013166 (2014).","journal-title":"Protein Sci."},{"key":"7929_CR46","doi-asserted-by":"publisher","first-page":"4169","DOI":"10.1042\/BCJ20210708","volume":"478","author":"D Kimanius","year":"2021","unstructured":"Kimanius, D., Dong, L., Sharov, G., Nakane, T. & Scheres, S. H. W. New tools for automated cryo-EM single-particle analysis in RELION-4.0. Biochem. J. 478, 4169\u20134185 (2021).","journal-title":"Biochem. J."},{"key":"7929_CR47","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1038\/nmeth.4193","volume":"14","author":"SQ Zheng","year":"2017","unstructured":"Zheng, S. Q. et al. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nat. Methods 14, 331\u2013332 (2017).","journal-title":"Nat. Methods"},{"key":"7929_CR48","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","volume":"192","author":"A Rohou","year":"2015","unstructured":"Rohou, A. & Grigorieff, N. CTFFIND4: fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192, 216\u2013221 (2015).","journal-title":"J. Struct. Biol."},{"key":"7929_CR49","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1038\/s41592-019-0580-y","volume":"16","author":"D Tegunov","year":"2019","unstructured":"Tegunov, D. & Cramer, P. Real-time cryo-electron microscopy data preprocessing with Warp. Nat. Methods 16, 1146\u20131152 (2019).","journal-title":"Nat. Methods"},{"key":"7929_CR50","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/nmeth.4169","volume":"14","author":"A Punjani","year":"2017","unstructured":"Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290\u2013296 (2017).","journal-title":"Nat. Methods"},{"key":"7929_CR51","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1107\/S2059798319011471","volume":"75","author":"D Liebschner","year":"2019","unstructured":"Liebschner, D. et al. Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix. Acta Crystallogr. D Struct. Biol. 75, 861\u2013877 (2019).","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"7929_CR52","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1002\/pro.3791","volume":"29","author":"A Casanal","year":"2020","unstructured":"Casanal, A., Lohkamp, B. & Emsley, P. Current developments in Coot for macromolecular model building of electron cryo-microscopy and crystallographic data. Protein Sci. 29, 1069\u20131078 (2020).","journal-title":"Protein Sci."},{"key":"7929_CR53","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1107\/S0907444909042073","volume":"66","author":"VB Chen","year":"2010","unstructured":"Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr. D Biol. Crystallogr. 66, 12\u201321 (2010).","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"7929_CR54","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1016\/S0263-7855(97)00009-X","volume":"14","author":"OS Smart","year":"1996","unstructured":"Smart, O. S., Neduvelil, J. G., Wang, X., Wallace, B. A. & Sansom, M. S. HOLE: a program for the analysis of the pore dimensions of ion channel structural models. J. Mol. Graph. 14, 354\u2013360, 376 (1996).","journal-title":"J. Mol. Graph."},{"key":"7929_CR55","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1016\/j.jmb.2007.05.022","volume":"372","author":"E Krissinel","year":"2007","unstructured":"Krissinel, E. & Henrick, K. Inference of macromolecular assemblies from crystalline state. J. Mol. Biol. 372, 774\u2013797 (2007).","journal-title":"J. Mol. Biol."},{"key":"7929_CR56","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1002\/pro.3943","volume":"30","author":"EF Pettersen","year":"2021","unstructured":"Pettersen, E. F. et al. UCSF ChimeraX: structure visualization for researchers, educators, and developers. Protein Sci. 30, 70\u201382 (2021).","journal-title":"Protein Sci."},{"key":"7929_CR57","doi-asserted-by":"publisher","first-page":"D543","DOI":"10.1093\/nar\/gkab1038","volume":"50","author":"Y Perez-Riverol","year":"2022","unstructured":"Perez-Riverol, Y. et al. The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences. Nucleic Acids Res. 50, D543\u2013D552 (2022).","journal-title":"Nucleic Acids Res."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41586-024-07929-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41586-024-07929-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41586-024-07929-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T04:48:35Z","timestamp":1730090915000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41586-024-07929-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,11]]},"references-count":57,"journal-issue":{"issue":"8035","published-print":{"date-parts":[[2024,10,24]]}},"alternative-id":["7929"],"URL":"https:\/\/doi.org\/10.1038\/s41586-024-07929-5","relation":{},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,11]]},"assertion":[{"value":"28 January 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 August 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}