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The marked plasticity of replication forks under replication stress ensures fork stability, damage tolerance and complete genome duplication\n                    <jats:sup>1<\/jats:sup>\n                    . Initiation and progression of replication forks occur in a three-dimensionally organized genome. DNA loop extrusion by the cohesin complex organizes the genome\n                    <jats:sup>2<\/jats:sup>\n                    and regulates the initiation and positioning of DNA replication origins\n                    <jats:sup>3,4<\/jats:sup>\n                    . Although transient interaction of sister forks was recently reported during unperturbed replication\n                    <jats:sup>5<\/jats:sup>\n                    , the functional relevance of fork contacts during replication stress and the role of cohesin in this context remain unknown. Here we show that cohesin-mediated loop extrusion rearranges nascent DNA contacts at stressed replication forks to promote genome stability. Using auxin-inducible degron\n                    <jats:sup>6<\/jats:sup>\n                    , separation-of-function mutants\n                    <jats:sup>7\u20139<\/jats:sup>\n                    and a newly developed Micro-C-based technique to capture chromatin contacts at nascent DNA (Repli-C), we found that loop-extruding cohesin accumulates at stalled replication forks, limiting sister-fork coupling in favour of inter-replicon contacts. This process promotes active fork slowing and reversal by preventing PRIMPOL action on single-stranded\u00a0DNA\n                    <jats:sup>1<\/jats:sup>\n                    . These findings show that the replication stress response is not merely an accumulation of individual regulatory events, but is topologically integrated across the genome through cohesin loop extrusion. While providing a new function for loop-extruding cohesin, our results indicate the potential impact on cancer therapy of frequent cohesin mutations in tumours\n                    <jats:sup>10<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41586-026-11034-0","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:04:13Z","timestamp":1789571053000},"page":"1057-1065","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cohesin reshapes replication fork contacts to aid fork slowing and reversal"],"prefix":"10.1038","volume":"658","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3686-2297","authenticated-orcid":false,"given":"Daniel","family":"Gonz\u00e1lez-Acosta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2284-9761","authenticated-orcid":false,"given":"Daniel","family":"Gim\u00e9nez-Llorente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4442-8238","authenticated-orcid":false,"given":"Melani","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moses","family":"Aouami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1472-032X","authenticated-orcid":false,"given":"Orhi","family":"Barroso-Gomila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3288-3496","authenticated-orcid":false,"given":"Javier","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9752-5932","authenticated-orcid":false,"given":"Ana","family":"Cuadrado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5251-3383","authenticated-orcid":false,"given":"Ana","family":"Losada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3847-8133","authenticated-orcid":false,"given":"Massimo","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"11034_CR1","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1038\/s41580-020-0257-5","volume":"21","author":"M Berti","year":"2020","unstructured":"Berti, M., Cortez, D. & Lopes, M. 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