{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T18:33:34Z","timestamp":1794508414345,"version":"4.9.1"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T00:00:00Z","timestamp":1578873600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T00:00:00Z","timestamp":1578873600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Biotechnol"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1038\/s41587-019-0374-x","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T12:02:48Z","timestamp":1578916968000},"page":"189-198","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":381,"title":["An integrated perfusion machine preserves injured human livers for 1 week"],"prefix":"10.1038","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9575-7094","authenticated-orcid":false,"given":"Dilmurodjon","family":"Eshmuminov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dustin","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7709-8024","authenticated-orcid":false,"given":"Lucia","family":"Bautista Borrego","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8038-4099","authenticated-orcid":false,"given":"Max","family":"Hefti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin J.","family":"Schuler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Hagedorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Muller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Mueller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Onder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Graf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0073-3637","authenticated-orcid":false,"given":"Achim","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Dutkowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Rudolf von Rohr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9916-7905","authenticated-orcid":false,"given":"Pierre-Alain","family":"Clavien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,13]]},"reference":[{"key":"374_CR1","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1038\/nbt.3889","volume":"35","author":"S Giwa","year":"2017","unstructured":"Giwa, S. et al. The promise of organ and tissue preservation to transform medicine. Nat. Biotechnol. 35, 530\u2013542 (2017).","journal-title":"Nat. Biotechnol."},{"key":"374_CR2","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1038\/s41587-019-0223-y","volume":"37","author":"RJ de Vries","year":"2019","unstructured":"de Vries, R. J. et al. Supercooling extends preservation time of human livers. Nat. Biotechnol. 37, 1131\u20131136 (2019).","journal-title":"Nat. Biotechnol."},{"key":"374_CR3","doi-asserted-by":"crossref","unstructured":"Buying time for transplants. Nat. Biotechnol. 35, 801 (2017).","DOI":"10.1038\/nbt.3971"},{"key":"374_CR4","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1111\/ajt.14687","volume":"18","author":"CJE Watson","year":"2018","unstructured":"Watson, C. J. E. et al. Observations on the ex situ perfusion of livers for transplantation. Am. J. Transplant. 18, 2005\u20132020 (2018).","journal-title":"Am. J. Transplant."},{"key":"374_CR5","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1038\/s41586-018-0047-9","volume":"557","author":"D Nasralla","year":"2018","unstructured":"Nasralla, D. et al. A randomized trial of normothermic preservation in liver transplantation. Nature 557, 50\u201356 (2018).","journal-title":"Nature"},{"key":"374_CR6","doi-asserted-by":"publisher","first-page":"1768","DOI":"10.1002\/bjs.10290","volume":"103","author":"D Eshmuminov","year":"2016","unstructured":"Eshmuminov, D. et al. Meta-analysis of associating liver partition with portal vein ligation and portal vein occlusion for two-stage hepatectomy. Br. J. Surg. 103, 1768\u20131782 (2016).","journal-title":"Br. J. Surg."},{"key":"374_CR7","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1097\/SLA.0b013e31824856f5","volume":"255","author":"AA Schnitzbauer","year":"2012","unstructured":"Schnitzbauer, A. A. et al. Right portal vein ligation combined with in situ splitting induces rapid left lateral liver lobe hypertrophy enabling 2-staged extended right hepatic resection in small-for-size settings. Ann. Surg. 255, 405\u2013414 (2012).","journal-title":"Ann. Surg."},{"key":"374_CR8","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1097\/SLA.0b013e318248577d","volume":"255","author":"E de Santibanes","year":"2012","unstructured":"de Santibanes, E. & Clavien, P. A. Playing Play-Doh to prevent postoperative liver failure: the \u201cALPPS\u201d approach. Ann. Surg. 255, 415\u2013417 (2012).","journal-title":"Ann. Surg."},{"key":"374_CR9","unstructured":"Lautt, W. W. In Colloquium Series on Integrated Systems Physiology: from Molecule to Function to Disease (Eds. Granger, D. N. and Granger, J. P.) (San Rafael, 2009)."},{"key":"374_CR10","unstructured":"Berg, J. M, Tymoczko, J. L. & Stryer, L. Biochemistry 5th edn (W. H. Freeman, 2002)."},{"key":"374_CR11","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1111\/tri.13306","volume":"31","author":"D Eshmuminov","year":"2018","unstructured":"Eshmuminov, D et al. Perfusion settings and additives in liver normothermic machine perfusion with red blood cells as oxygen carrier. A systematic review of human and porcine perfusion protocols. Transplant Int. 31, 956\u2013969 (2018).","journal-title":"Transplant Int."},{"key":"374_CR12","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1002\/lt.24972","volume":"24","author":"Q Liu","year":"2018","unstructured":"Liu, Q. et al. Lipid metabolism and functional assessment of discarded human livers with steatosis undergoing 24\u2009h of normothermic machine perfusion. Liver Transpl. 24, 233\u2013245 (2018).","journal-title":"Liver Transpl."},{"key":"374_CR13","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1097\/01.MAT.0000129320.57362.db","volume":"50","author":"A Baba","year":"2004","unstructured":"Baba, A. et al. Microcirculation of the bulbar conjunctiva in the goat implanted with a total artificial heart: effects of pulsatile and nonpulsatile flow. ASAIO J. 50, 321\u2013327 (2004).","journal-title":"ASAIO J."},{"key":"374_CR14","doi-asserted-by":"publisher","first-page":"M724","DOI":"10.1097\/00002480-199609000-00083","volume":"42","author":"Y Orime","year":"1996","unstructured":"Orime, Y. et al. The role of pulsatility in end-organ microcirculation after cardiogenic shock. ASAIO J. 42, M724\u2013M729 (1996).","journal-title":"ASAIO J."},{"key":"374_CR15","doi-asserted-by":"publisher","first-page":"2046","DOI":"10.1016\/j.athoracsur.2012.05.065","volume":"94","author":"MP O\u2019Neil","year":"2012","unstructured":"O\u2019Neil, M. P., Fleming, J. C., Badhwar, A. & Guo, L. R. Pulsatile versus nonpulsatile flow during cardiopulmonary bypass: microcirculatory and systemic effects. Ann. Thorac. Surg. 94, 2046\u20132053 (2012).","journal-title":"Ann. Thorac. Surg."},{"key":"374_CR16","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.1152\/japplphysiol.01191.2011","volume":"112","author":"NJ Koning","year":"2012","unstructured":"Koning, N. J. et al. Pulsatile flow during cardiopulmonary bypass preserves postoperative microcirculatory perfusion irrespective of systemic hemodynamics. J. Appl. Physiol. 112, 1727\u20131734 (2012).","journal-title":"J. Appl. Physiol."},{"key":"374_CR17","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/j.jhep.2015.10.006","volume":"64","author":"European Association for the Study of the Liver.","year":"2016","unstructured":"European Association for the Study of the Liver. EASL clinical practice guidelines: liver transplantation. J. Hepatol. 64, 433\u2013485 (2016).","journal-title":"J. Hepatol."},{"key":"374_CR18","doi-asserted-by":"publisher","DOI":"10.1186\/s40635-017-0135-4","volume":"5","author":"I Attaye","year":"2017","unstructured":"Attaye, I. et al. The effects of hyperoxia on microvascular endothelial cell proliferation and production of vaso-active substances. Intensive Care Med. Exp. 5, 22 (2017).","journal-title":"Intensive Care Med. Exp."},{"key":"374_CR19","first-page":"864","volume":"41","author":"S Dallinger","year":"2000","unstructured":"Dallinger, S. et al. Endothelin-1 contributes to hyperoxia-induced vasoconstriction in the human retina. Invest. Ophthalmol. Vis. Sci. 41, 864\u2013869 (2000).","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"374_CR20","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1002\/lt.25007","volume":"24","author":"Q Liu","year":"2018","unstructured":"Liu, Q. et al. Ex situ 86-hour liver perfusion: pushing the boundary of organ preservation. Liver Transpl. 24, 557\u2013561 (2018).","journal-title":"Liver Transpl."},{"key":"374_CR21","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1177\/039139889301601010","volume":"16","author":"P Neuhaus","year":"1993","unstructured":"Neuhaus, P. & Blumhardt, G. Extracorporeal liver perfusion: applications of an improved model for experimental studies of the liver. Int. J. Artif. Organs 16, 729\u2013739 (1993).","journal-title":"Int. J. Artif. Organs"},{"key":"374_CR22","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1097\/SLA.0b013e3181bcb1ee","volume":"250","author":"O de Rougemont","year":"2009","unstructured":"de Rougemont, O. et al. One hour hypothermic oxygenated perfusion (HOPE) protects nonviable liver allografts donated after cardiac death. Ann. Surg. 250, 674\u2013683 (2009).","journal-title":"Ann. Surg."},{"key":"374_CR23","doi-asserted-by":"publisher","first-page":"44","DOI":"10.5339\/gcsp.2013.44","volume":"2013","author":"H Saad","year":"2013","unstructured":"Saad, H. & Aladawy, M. Temperature management in cardiac surgery. Glob. Cardiol. Sci. Pract. 2013, 44\u201362 (2013).","journal-title":"Glob. Cardiol. Sci. Pract."},{"key":"374_CR24","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1097\/00007890-199205000-00001","volume":"53","author":"PA Clavien","year":"1992","unstructured":"Clavien, P. A., Harvey, P. R. & Strasberg, S. M. Preservation and reperfusion injuries in liver allografts. An overview and synthesis of current studies. Transplantation 53, 957\u2013978 (1992).","journal-title":"Transplantation"},{"key":"374_CR25","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1016\/j.cmet.2015.12.009","volume":"23","author":"ET Chouchani","year":"2016","unstructured":"Chouchani, E. T. et al. A unifying mechanism for mitochondrial superoxide production during ischemia\u2013reperfusion injury. Cell Metab. 23, 254\u2013263 (2016).","journal-title":"Cell Metab."},{"key":"374_CR26","doi-asserted-by":"publisher","first-page":"1280","DOI":"10.1053\/jhep.2000.20528","volume":"32","author":"M Selzner","year":"2000","unstructured":"Selzner, M., Rudiger, H. A., Sindram, D., Madden, J. & Clavien, P. A. Mechanisms of ischemic injury are different in the steatotic and normal rat liver. Hepatology 32, 1280\u20131288 (2000).","journal-title":"Hepatology"},{"key":"374_CR27","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1097\/SLA.0b013e31815c2a3f","volume":"246","author":"L McCormack","year":"2007","unstructured":"McCormack, L. et al. Use of severely steatotic grafts in liver transplantation: a matched case-control study. Ann Surg. 246, 940\u2013946 (2007).","journal-title":"Ann Surg."},{"key":"374_CR28","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.1016\/j.hpb.2019.01.004","volume":"21","author":"X Muller","year":"2019","unstructured":"Muller, X et al. Can hypothermic oxygenated perfusion (HOPE) rescue futile DCD liver grafts? HPB 21, 1156\u20131165 (2019).","journal-title":"HPB"},{"key":"374_CR29","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1097\/SLA.0000000000002477","volume":"267","author":"X Muller","year":"2018","unstructured":"Muller, X. et al. Defining benchmarks in liver transplantation: a multicenter outcome analysis determining best achievable results. Ann. Surg. 267, 419\u2013425 (2018).","journal-title":"Ann. Surg."},{"key":"374_CR30","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1097\/TP.0000000000002500","volume":"103","author":"APM Matton","year":"2019","unstructured":"Matton, A. P. M. et al. Biliary bicarbonate, pH, and glucose are suitable biomarkers of biliary viability during ex situ normothermic machine perfusion of human donor livers. Transplantation 103, 1405\u20131413 (2019).","journal-title":"Transplantation"},{"key":"374_CR31","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1093\/clinchem\/43.8.1539","volume":"43","author":"A Shaked","year":"1997","unstructured":"Shaked, A., Nunes, F. A., Olthoff, K. M. & Lucey, M. R. Assessment of liver function: pre- and peritransplant evaluation. Clin. Chem. 43, 1539\u20131545 (1997).","journal-title":"Clin. Chem."},{"key":"374_CR32","doi-asserted-by":"publisher","first-page":"1298","DOI":"10.1002\/lt.25290","volume":"24","author":"SA Karangwa","year":"2018","unstructured":"Karangwa, S. A. et al. Production of physiologically relevant quantities of hemostatic proteins during ex situ normothermic machine perfusion of human livers. Liver Transpl. 24, 1298\u20131302 (2018).","journal-title":"Liver Transpl."},{"key":"374_CR33","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1590\/s1679-45082016rw3585","volume":"14","author":"DB Neves","year":"2016","unstructured":"Neves, D. B., Rusi, M. B., Diaz, L. G. & Salvalaggio, P. Primary graft dysfunction of the liver: definitions, diagnostic criteria and risk factors. Einstein 14, 567\u2013572 (2016).","journal-title":"Einstein"},{"key":"374_CR34","doi-asserted-by":"crossref","first-page":"e017733","DOI":"10.1136\/bmjopen-2017-017733","volume":"7","author":"RW Laing","year":"2017","unstructured":"Laing, R. W. et al. Viability testing and transplantation of marginal livers (VITTAL) using normothermic machine perfusion: study protocol for an open-label, non-randomised, prospective, single-arm trial. BMJ Open 7, e017733 (2017).","journal-title":"BMJ Open"},{"key":"374_CR35","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1016\/j.jhep.2014.04.031","volume":"61","author":"CJ Verhoeven","year":"2014","unstructured":"Verhoeven, C. J. et al. Biomarkers to assess graft quality during conventional and machine preservation in liver transplantation. J. Hepatol. 61, 672\u2013684 (2014).","journal-title":"J. Hepatol."},{"key":"374_CR36","doi-asserted-by":"publisher","first-page":"2991","DOI":"10.1111\/ajt.15395","volume":"19","author":"I Linares-Cervantes","year":"2019","unstructured":"Linares-Cervantes, I et al. Predictor parameters of liver viability during porcine normothermic ex situ liver perfusion in a model of liver transplantation with marginal grafts. Am. J. Transplant. 19, 2991\u20133005 (2019).","journal-title":"Am. J. Transplant."},{"key":"374_CR37","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1002\/cld.540","volume":"7","author":"A Fedoravicius","year":"2016","unstructured":"Fedoravicius, A. & Charlton, M. Abnormal liver tests after liver transplantation. Clin. Liver. Dis. 7, 73\u201379 (2016).","journal-title":"Clin. Liver. Dis."},{"key":"374_CR38","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/j.cell.2017.04.015","volume":"169","author":"F Sinturel","year":"2017","unstructured":"Sinturel, F. et al. Diurnal oscillations in liver mass and cell size accompany ribosome assembly cycles. Cell 169, 651\u2013663 (2017).","journal-title":"Cell"},{"key":"374_CR39","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1002\/lt.25036","volume":"24","author":"P Dutkowski","year":"2018","unstructured":"Dutkowski, P. & Clavien, P. A. Uploading cellular batteries: caring for mitochondria is key. Liver Transpl. 24, 462\u2013464 (2018).","journal-title":"Liver Transpl."},{"key":"374_CR40","doi-asserted-by":"publisher","first-page":"1024","DOI":"10.1136\/gut.2004.053850","volume":"54","author":"ME Guicciardi","year":"2005","unstructured":"Guicciardi, M. E. & Gores, G. J. Apoptosis: a mechanism of acute and chronic liver injury. Gut 54, 1024\u20131033 (2005).","journal-title":"Gut"}],"container-title":["Nature Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41587-019-0374-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41587-019-0374-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41587-019-0374-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T17:29:28Z","timestamp":1733938168000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41587-019-0374-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,13]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["374"],"URL":"https:\/\/doi.org\/10.1038\/s41587-019-0374-x","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.737204022.793572011","asserted-by":"object"}]},"ISSN":["1087-0156","1546-1696"],"issn-type":[{"value":"1087-0156","type":"print"},{"value":"1546-1696","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,13]]},"assertion":[{"value":"23 December 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 November 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 January 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"ETH (Swiss Federal Institute of Technology in Zurich) and the University of Zurich (D.E., D.B., L.B.B., M.H., M.J.S., X.M., P.D., R. G., P.R.v.R. and P.-A.C.) have applied for a patent on this new perfusion technology (PCT\/EP2019\/051252).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}