{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:23:18Z","timestamp":1792790598876,"version":"4.5.0"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T00:00:00Z","timestamp":1628121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T00:00:00Z","timestamp":1628121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100000925","name":"Department of Health | National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1175568"],"award-info":[{"award-number":["1175568"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005909","name":"University of California Institute for Mexico and the United States","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005909","id-type":"DOI","asserted-by":"publisher"}]},{"name":"El Consejo Nacional de Ciencia y Tecnologia"},{"DOI":"10.13039\/100000060","name":"U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["F30AI143401"],"award-info":[{"award-number":["F30AI143401"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008072","name":"Gladstone Institutes","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008072","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chan Zuckerberg Biohub"},{"DOI":"10.13039\/100000002","name":"U.S. Department of Health & Human Services | National Institutes of Health","doi-asserted-by":"publisher","award":["1S10OD020062-01"],"award-info":[{"award-number":["1S10OD020062-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"U.S. Department of Health & Human Services | National Institutes of Health","doi-asserted-by":"publisher","award":["5R61AI140465-03"],"award-info":[{"award-number":["5R61AI140465-03"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"U.S. Department of Health & Human Services | National Institutes of Health","doi-asserted-by":"publisher","award":["5R61AI140465-03"],"award-info":[{"award-number":["5R61AI140465-03"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"U.S. Department of Health & Human Services | National Institutes of Health","doi-asserted-by":"publisher","award":["DP5OD021369"],"award-info":[{"award-number":["DP5OD021369"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"U.S. Department of Health & Human Services | National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM131073"],"award-info":[{"award-number":["R01GM131073"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"U.S. Department of Health & Human Services | National Institutes of Health","doi-asserted-by":"publisher","award":["5R61AI140465-03"],"award-info":[{"award-number":["5R61AI140465-03"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"name":"U.S. Department of Health & Human Services | National Institutes of Health","id":[{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"name":"U.S. Department of Health & Human Services | National Institutes of Health","id":[{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"name":"U.S. Department of Health & Human Services | National Institutes of Health","id":[{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"name":"U.S. Department of Health & Human Services | National Institutes of Health","id":[{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000185","name":"United States Department of Defense | Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["N66001-20-2-4033"],"award-info":[{"award-number":["N66001-20-2-4033"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000185","name":"United States Department of Defense | Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["N66001-20-2-4033"],"award-info":[{"award-number":["N66001-20-2-4033"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]},{"name":"U.S. Department of Health & Human Services | National Institutes of Health","id":[{"id":"https:\/\/ror.org\/033jnv181","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Chem Biol"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1038\/s41589-021-00842-2","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T12:05:27Z","timestamp":1628165127000},"page":"982-988","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":258,"title":["Accelerated RNA detection using tandem CRISPR nucleases"],"prefix":"10.1038","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1297-7102","authenticated-orcid":false,"given":"Tina Y.","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9007-6273","authenticated-orcid":false,"given":"Gavin J.","family":"Knott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan C. J.","family":"Smock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7739-2079","authenticated-orcid":false,"given":"John J.","family":"Desmarais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sungmin","family":"Son","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Bhuiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrutee","family":"Jakhanwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2526-2392","authenticated-orcid":false,"given":"Noam","family":"Prywes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shreeya","family":"Agrawal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"D\u00edaz de Le\u00f3n Derby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3639-4588","authenticated-orcid":false,"given":"Neil A.","family":"Switz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxim","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emeric J.","family":"Charles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brittney W.","family":"Thornton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6710-1506","authenticated-orcid":false,"given":"Parinaz","family":"Fozouni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Shu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6631-2184","authenticated-orcid":false,"given":"Stephanie I.","family":"Stephens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Renuka","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6981-1547","authenticated-orcid":false,"given":"Amanda","family":"Mok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony T.","family":"Iavarone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7221-7896","authenticated-orcid":false,"given":"Arturo M.","family":"Escajeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"McIntosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shineui","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2400-5511","authenticated-orcid":false,"given":"Eli J.","family":"Dugan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"IGI Testing Consortium","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Lin-Shiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth C.","family":"Stahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor A.","family":"Tsuchida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica A.","family":"Moehle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petros","family":"Giannikopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"McElroy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shana","family":"McDevitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arielle","family":"Zur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iman","family":"Sylvain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"Ciling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisha","family":"Baldwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9870-6196","authenticated-orcid":false,"given":"Katherine S.","family":"Pollard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming X.","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5697-1274","authenticated-orcid":false,"given":"Melanie","family":"Ott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1890-5364","authenticated-orcid":false,"given":"Daniel A.","family":"Fletcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3223-3426","authenticated-orcid":false,"given":"Liana F.","family":"Lareau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9380-2648","authenticated-orcid":false,"given":"Patrick D.","family":"Hsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0042-2257","authenticated-orcid":false,"given":"David F.","family":"Savage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9161-999X","authenticated-orcid":false,"given":"Jennifer A.","family":"Doudna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,5]]},"reference":[{"key":"842_CR1","doi-asserted-by":"publisher","first-page":"eabd5393","DOI":"10.1126\/sciadv.abd5393","volume":"7","author":"DB Larremore","year":"2021","unstructured":"Larremore, D. B. et al. Test sensitivity is secondary to frequency and turnaround time for COVID-19 screening. Sci. Adv. 7, eabd5393 (2021).","journal-title":"Sci. Adv."},{"key":"842_CR2","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1038\/s41564-020-0761-6","volume":"5","author":"CBF Vogels","year":"2020","unstructured":"Vogels, C. B. F. et al. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT\u2013qPCR primer\u2013probe sets. Nat. Microbiol. 5, 1299\u20131305 (2020).","journal-title":"Nat. Microbiol."},{"key":"842_CR3","doi-asserted-by":"publisher","first-page":"e120","DOI":"10.1056\/NEJMp2025631","volume":"383","author":"MJ Mina","year":"2020","unstructured":"Mina, M. J., Parker, R. & Larremore, D. B. Rethinking COVID-19 test sensitivity\u2014a strategy for containment. N. Engl. J. Med. 383, e120 (2020).","journal-title":"N. Engl. J. Med."},{"key":"842_CR4","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1126\/science.aat5011","volume":"361","author":"GJ Knott","year":"2018","unstructured":"Knott, G. J. & Doudna, J. A. CRISPR\u2013Cas guides the future of genetic engineering. Science 361, 866\u2013869 (2018).","journal-title":"Science"},{"key":"842_CR5","doi-asserted-by":"publisher","first-page":"14473","DOI":"10.1074\/jbc.REV120.007034","volume":"295","author":"TY Liu","year":"2020","unstructured":"Liu, T. Y. & Doudna, J. A. Chemistry of class 1 CRISPR\u2013Cas effectors: binding, editing, and regulation. J. Biol. Chem. 295, 14473\u201314487 (2020).","journal-title":"J. Biol. Chem."},{"key":"842_CR6","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1038\/s41579-019-0299-x","volume":"18","author":"KS Makarova","year":"2020","unstructured":"Makarova, K. S. et al. Evolutionary classification of CRISPR\u2013Cas systems: a burst of class 2 and derived variants. Nat. Rev. Microbiol. 18, 67\u201383 (2020).","journal-title":"Nat. Rev. Microbiol."},{"key":"842_CR7","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1038\/nature19802","volume":"538","author":"A East-Seletsky","year":"2016","unstructured":"East-Seletsky, A. et al. Two distinct RNase activities of CRISPR\u2013C2c2 enable guide-RNA processing and RNA detection. Nature 538, 270\u2013273 (2016).","journal-title":"Nature"},{"key":"842_CR8","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1126\/science.aam9321","volume":"356","author":"JS Gootenberg","year":"2017","unstructured":"Gootenberg, J. S. et al. Nucleic acid detection with CRISPR\u2013Cas13a\/C2c2. Science 356, 438\u2013442 (2017).","journal-title":"Science"},{"key":"842_CR9","doi-asserted-by":"publisher","unstructured":"Steens, J. A. et al. SCOPE: flexible targeting and stringent CARF activation enables type III CRISPR\u2013Cas diagnostics. Preprint at bioRxiv https:\/\/doi.org\/10.1101\/2021.02.01.429135 (2021).","DOI":"10.1101\/2021.02.01.429135"},{"key":"842_CR10","doi-asserted-by":"publisher","first-page":"100319","DOI":"10.1016\/j.xcrm.2021.100319","volume":"2","author":"A Santiago-Frangos","year":"2021","unstructured":"Santiago-Frangos, A. et al. Intrinsic signal amplification by type-III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnostic. Cell Rep. Med. 2, 100319 (2021).","journal-title":"Cell Rep. Med."},{"key":"842_CR11","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1126\/science.aao0100","volume":"357","author":"M Kazlauskiene","year":"2017","unstructured":"Kazlauskiene, M., Kostiuk, G., Venclovas, \u010c., Tamulaitis, G. & Siksnys, V. A cyclic oligonucleotide signaling pathway in type III CRISPR\u2013Cas systems. Science 357, 605\u2013609 (2017).","journal-title":"Science"},{"key":"842_CR12","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1038\/nature23467","volume":"548","author":"O Niewoehner","year":"2017","unstructured":"Niewoehner, O. et al. Type III CRISPR\u2013Cas systems produce cyclic oligoadenylate second messengers. Nature 548, 543\u2013548 (2017).","journal-title":"Nature"},{"key":"842_CR13","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/j.molcel.2017.04.008","volume":"66","author":"A East-Seletsky","year":"2017","unstructured":"East-Seletsky, A., O\u2019Connell, M. R., Burstein, D., Knott, G. J. & Doudna, J. A. RNA targeting by functionally orthogonal type VI-A CRISPR\u2013Cas enzymes. Mol. Cell 66, 373\u2013383 (2017).","journal-title":"Mol. Cell"},{"key":"842_CR14","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1006\/mcpr.2002.0415","volume":"16","author":"K Nagamine","year":"2002","unstructured":"Nagamine, K., Hase, T. & Notomi, T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell. Probes 16, 223\u2013229 (2002).","journal-title":"Mol. Cell. Probes"},{"key":"842_CR15","doi-asserted-by":"publisher","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","volume":"4","author":"O Piepenburg","year":"2006","unstructured":"Piepenburg, O., Williams, C. H., Stemple, D. L. & Armes, N. A. DNA detection using recombination proteins. PLoS Biol. 4, e204 (2006).","journal-title":"PLoS Biol."},{"key":"842_CR16","doi-asserted-by":"publisher","first-page":"1492","DOI":"10.1056\/NEJMc2026172","volume":"383","author":"J Joung","year":"2020","unstructured":"Joung, J. et al. Detection of SARS-CoV-2 with SHERLOCK one-pot testing. N. Engl. J. Med. 383, 1492\u20131494 (2020).","journal-title":"N. Engl. J. Med."},{"key":"842_CR17","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","volume":"38","author":"JP Broughton","year":"2020","unstructured":"Broughton, J. P. et al. CRISPR\u2013Cas12-based detection of SARS-CoV-2. Nat. Biotechnol. 38, 870\u2013874 (2020).","journal-title":"Nat. Biotechnol."},{"key":"842_CR18","doi-asserted-by":"publisher","unstructured":"Joung, J. et al. Point-of-care testing for COVID-19 using SHERLOCK diagnostics. Preprint at medRxiv https:\/\/doi.org\/10.1101\/2020.05.04.20091231 (2020).","DOI":"10.1101\/2020.05.04.20091231"},{"key":"842_CR19","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19097-x","volume":"11","author":"J Arizti-Sanz","year":"2020","unstructured":"Arizti-Sanz, J. et al. Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2. Nat. Commun. 11, 5921 (2020).","journal-title":"Nat. Commun."},{"key":"842_CR20","unstructured":"Zhang, F., Abudayyeh, O. O., & Gootenberg, J. S. A protocol for detection of COVID-19 using CRISPR diagnostics. https:\/\/www.broadinstitute.org\/files\/publications\/special\/COVID-19%20detection%20(updated).pdf (2020)."},{"key":"842_CR21","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.cell.2020.12.001","volume":"184","author":"P Fozouni","year":"2021","unstructured":"Fozouni, P. et al. Amplification-free detection of SARS-CoV-2 with CRISPR\u2013Cas13a and mobile phone microscopy. Cell 184, 323\u2013333 (2021).","journal-title":"Cell"},{"key":"842_CR22","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1126\/science.aaq0179","volume":"360","author":"JS Gootenberg","year":"2018","unstructured":"Gootenberg, J. S. et al. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science 360, 439\u2013444 (2018).","journal-title":"Science"},{"key":"842_CR23","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15334-5","volume":"11","author":"C Garcia-Doval","year":"2020","unstructured":"Garcia-Doval, C. et al. Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6. Nat. Commun. 11, 1596 (2020).","journal-title":"Nat. Commun."},{"key":"842_CR24","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1038\/s41586-018-0557-5","volume":"562","author":"JS Athukoralage","year":"2018","unstructured":"Athukoralage, J. S., Rouillon, C., Graham, S., Gr\u00fcschow, S. & White, M. F. Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate. Nature 562, 277\u2013280 (2018).","journal-title":"Nature"},{"key":"842_CR25","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1016\/j.molcel.2019.06.014","volume":"75","author":"N Jia","year":"2019","unstructured":"Jia, N., Jones, R., Yang, G., Ouerfelli, O. & Patel, D. J. CRISPR\u2013Cas III-A Csm6 CARF domain is a ring nuclease triggering stepwise cA4 cleavage with ApA>p formation terminating RNase activity. Mol. Cell 75, 944\u2013956 (2019).","journal-title":"Mol. Cell"},{"key":"842_CR26","doi-asserted-by":"publisher","first-page":"2894","DOI":"10.1016\/j.jmb.2019.04.041","volume":"431","author":"JS Athukoralage","year":"2019","unstructured":"Athukoralage, J. S., Graham, S., Gr\u00fcschow, S., Rouillon, C. & White, M. F. A type III CRISPR ancillary ribonuclease degrades its cyclic oligoadenylate activator. J. Mol. Biol. 431, 2894\u20132899 (2019).","journal-title":"J. Mol. Biol."},{"key":"842_CR27","doi-asserted-by":"publisher","first-page":"9204","DOI":"10.1093\/nar\/gkaa634","volume":"48","author":"D Smalakyte","year":"2020","unstructured":"Smalakyte, D. et al. Type III-A CRISPR-associated protein Csm6 degrades cyclic hexa-adenylate activator using both CARF and HEPN domains. Nucleic Acids Res. 48, 9204\u20139217 (2020).","journal-title":"Nucleic Acids Res."},{"key":"842_CR28","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10780-2","volume":"10","author":"TY Liu","year":"2019","unstructured":"Liu, T. Y., Liu, J.-J., Aditham, A. J., Nogales, E. & Doudna, J. A. Target preference of type III-A CRISPR\u2013Cas complexes at the transcription bubble. Nat. Commun. 10, 3001 (2019).","journal-title":"Nat. Commun."},{"key":"842_CR29","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1261\/rna.054098.115","volume":"22","author":"O Niewoehner","year":"2016","unstructured":"Niewoehner, O. & Jinek, M. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22, 318\u2013329 (2016).","journal-title":"RNA"},{"key":"842_CR30","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1038\/s41587-020-0583-3","volume":"38","author":"IGI Testing Consortium","year":"2020","unstructured":"IGI Testing Consortium. Blueprint for a pop-up SARS-CoV-2 testing lab. Nat. Biotechnol. 38, 791\u2013797 (2020).","journal-title":"Nat. Biotechnol."},{"key":"842_CR31","doi-asserted-by":"publisher","first-page":"2663","DOI":"10.1093\/cid\/ciaa638","volume":"71","author":"J Bullard","year":"2020","unstructured":"Bullard, J. et al. Predicting infectious severe acute respiratory syndrome coronavirus 2 from diagnostic samples. Clin. Infect. Dis. 71, 2663\u20132666 (2020).","journal-title":"Clin. Infect. Dis."},{"key":"842_CR32","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1038\/s41586-020-2279-8","volume":"582","author":"CM Ackerman","year":"2020","unstructured":"Ackerman, C. M. et al. Massively multiplexed nucleic acid detection using Cas13. Nature 582, 277\u2013282 (2020).","journal-title":"Nature"},{"key":"842_CR33","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-14222-x","volume":"11","author":"SA McMahon","year":"2020","unstructured":"McMahon, S. A. et al. Structure and mechanism of a type III CRISPR defence DNA nuclease activated by cyclic oligoadenylate. Nat. Commun. 11, 500 (2020).","journal-title":"Nat. Commun."},{"key":"842_CR34","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1016\/j.molcel.2019.12.010","volume":"77","author":"RK Lau","year":"2020","unstructured":"Lau, R. K. et al. Structure and mechanism of a cyclic trinucleotide-activated bacterial endonuclease mediating bacteriophage immunity. Mol. Cell 77, 723\u2013733 (2020).","journal-title":"Mol. Cell"},{"key":"842_CR35","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1038\/s41586-021-03206-x","volume":"590","author":"JT Rost\u00f8l","year":"2021","unstructured":"Rost\u00f8l, J. T. et al. The Card1 nuclease provides defence during type III CRISPR immunity. Nature 590, 624\u2013629 (2021).","journal-title":"Nature"},{"key":"842_CR36","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12244-z","volume":"10","author":"R Molina","year":"2019","unstructured":"Molina, R. et al. Structure of Csx1\u2013cOA4 complex reveals the basis of RNA decay in type III-B CRISPR\u2013Cas. Nat. Commun. 10, 4302 (2019).","journal-title":"Nat. Commun."},{"key":"842_CR37","doi-asserted-by":"publisher","first-page":"e55852","DOI":"10.7554\/eLife.55852","volume":"9","author":"JS Athukoralage","year":"2020","unstructured":"Athukoralage, J. S. et al. The dynamic interplay of host and viral enzymes in type III CRISPR-mediated cyclic nucleotide signalling. eLife 9, e55852 (2020).","journal-title":"eLife"},{"key":"842_CR38","doi-asserted-by":"publisher","first-page":"1903661","DOI":"10.1002\/advs.201903661","volume":"7","author":"T Zhou","year":"2020","unstructured":"Zhou, T. et al. CRISPR\/Cas13a powered portable electrochemiluminescence chip for ultrasensitive and specific miRNA detection. Adv. Sci. 7, 1903661 (2020).","journal-title":"Adv. Sci."},{"key":"842_CR39","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1038\/nmeth.3323","volume":"12","author":"K Smith","year":"2015","unstructured":"Smith, K. et al. CIDRE: an illumination-correction method for optical microscopy. Nat. Methods 12, 404\u2013406 (2015).","journal-title":"Nat. Methods"},{"key":"842_CR40","doi-asserted-by":"publisher","unstructured":"Desmarais, J. J. & Bhuiya, A. jackdesmarais\/FIND-IT-paper: v1.0 (Version paper_code). Zenodo https:\/\/doi.org\/10.5281\/zenodo.4921924 (2021).","DOI":"10.5281\/zenodo.4921924"}],"updated-by":[{"DOI":"10.1038\/s41589-021-00882-8","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T00:00:00Z","timestamp":1633478400000}}],"container-title":["Nature Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41589-021-00842-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41589-021-00842-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41589-021-00842-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T17:38:27Z","timestamp":1733938707000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41589-021-00842-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,5]]},"references-count":40,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["842"],"URL":"https:\/\/doi.org\/10.1038\/s41589-021-00842-2","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.03.19.21253328","asserted-by":"object"}]},"ISSN":["1552-4450","1552-4469"],"issn-type":[{"value":"1552-4450","type":"print"},{"value":"1552-4469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,5]]},"assertion":[{"value":"11 May 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 June 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 August 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 October 2021","order":4,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Correction","order":5,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"A Correction to this paper has been published:","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"https:\/\/doi.org\/10.1038\/s41589-021-00882-8","URL":"https:\/\/doi.org\/10.1038\/s41589-021-00882-8","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"D.F.S. is a cofounder of Scribe Therapeutics and a scientific advisory board member of Scribe Therapeutics and Mammoth Biosciences. P.D.H. is a cofounder of Spotlight Therapeutics and Moment Biosciences and serves on the board of directors and scientific advisory board and is a scientific advisory board member to Vial Health and Serotiny. The Regents of the University of California have patents issued and\/or pending for CRISPR technologies on which J.A.D., T.Y.L., P.D.H., N.P., D.F.S., D.C.J.S., S.K., B.W.T., E.J.C., S.J. and G.J.K. are inventors. M.O., P.F., G.R.K., D.A.F., S.S. and N.A.S. have also filed patent applications related to this work. J.A.D. is a cofounder of Caribou Biosciences, Editas Medicine, Scribe Therapeutics, Intellia Therapeutics and Mammoth Biosciences. J.A.D. is a scientific advisory board member of Caribou Biosciences, Intellia Therapeutics, eFFECTOR Therapeutics, Scribe Therapeutics, Mammoth Biosciences, Synthego, Algen Biotechnologies, Felix Biosciences and Inari. J.A.D. is a Director at Johnson & Johnson and has research projects sponsored by Biogen, Pfizer, AppleTree Partners and Roche.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}