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Using CRISPR\u2013Cas9 gene editing, we were able to achieve a reduction of ~90\u201395% in the expression of these genes.\n                    <jats:italic>FOXP3<\/jats:italic>\n                    knockout in tonsil T cells led to production of antibodies against a variety of autoantigens and increased the affinity of influenza-specific antibodies. By contrast,\n                    <jats:italic>GZMB<\/jats:italic>\n                    knockout resulted in an increase in follicular helper T cells, consistent with the ablation of CD8\n                    <jats:sup>+<\/jats:sup>\n                    regulatory T cells observed in mouse models, and a marked expansion of autoreactive CD8\n                    <jats:sup>+<\/jats:sup>\n                    and CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells. These findings highlight the distinct yet complementary roles of CD8\n                    <jats:sup>+<\/jats:sup>\n                    and CD4\n                    <jats:sup>+<\/jats:sup>\n                    regulatory T cells in regulating cellular and humoral responses to prevent autoimmunity.\n                  <\/jats:p>","DOI":"10.1038\/s41590-024-02062-x","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T05:07:32Z","timestamp":1736744852000},"page":"230-239","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Differential roles of human CD4+ and CD8+ regulatory T cells in controlling self-reactive immune responses"],"prefix":"10.1038","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5310-5426","authenticated-orcid":false,"given":"Xin","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2774-2157","authenticated-orcid":false,"given":"Mustafa","family":"Ghanizada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0658-3652","authenticated-orcid":false,"given":"Vamsee","family":"Mallajosyula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Sola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robson","family":"Capasso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karan Raj","family":"Kathuria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6868-657X","authenticated-orcid":false,"given":"Mark M.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,13]]},"reference":[{"key":"2062_CR1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1159\/000443526","volume":"8","author":"P Valent","year":"2016","unstructured":"Valent, P. et al. 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