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A comprehensive single-cell RNA-sequencing and T cell receptor-sequencing analysis of the cerebrospinal fluid and blood from individuals in the MS and control cohorts revealed a subset of 23 highly expanded and activated CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell clonotypes that were enriched predominantly in the cerebrospinal fluid in the MS cohort. Using unbiased and targeted antigen discovery approaches, six CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell clonotypes recognizing Epstein\u2013Barr virus (EBV) antigens and multiple novel mimotopes were identified. Although the majority of mimotopes did not elicit functional responses, three of the expanded CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell receptors from patients with MS were reactive to EBV. EBV DNA and transcripts were detected in cerebrospinal fluid, including in patients with MS who had highly expanded EBV-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. These findings shed vital insight into the role of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in MS and support an important role of EBV in MS immunopathology.\n                  <\/jats:p>","DOI":"10.1038\/s41590-025-02412-3","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T10:04:00Z","timestamp":1770285840000},"page":"490-502","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Antigen specificity of clonally enriched CD8+ T cells in multiple 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