{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:23:29Z","timestamp":1792790609765,"version":"4.5.0"},"reference-count":67,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","award":["13549"],"award-info":[{"award-number":["13549"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NWO Dutch Research Council","id":[{"id":"https:\/\/ror.org\/04jsz6e67","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009425","name":"Landsteiner Foundation for Blood Transfusion Research","doi-asserted-by":"publisher","award":["LSBR 22860"],"award-info":[{"award-number":["LSBR 22860"]}],"id":[{"id":"10.13039\/100009425","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Immunol"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1038\/s41590-026-02481-y","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T09:04:48Z","timestamp":1774947888000},"page":"975-984","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Blood vessel-resident macrophages safeguard blood and vessel integrity in zebrafish"],"prefix":"10.1038","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4843-3019","authenticated-orcid":false,"given":"Bart","family":"Weijts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8757-9611","authenticated-orcid":false,"given":"Jeroen A. A.","family":"Demmers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9895-9046","authenticated-orcid":false,"given":"Catherine","family":"Robin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,31]]},"reference":[{"key":"2481_CR1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-017-0392-4","volume":"15","author":"S Gordon","year":"2017","unstructured":"Gordon, S. & Pl\u00fcddemann, A. Tissue macrophages: heterogeneity and functions. BMC Biol. 15, 53 (2017).","journal-title":"BMC Biol."},{"key":"2481_CR2","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1038\/s41577-023-00848-y","volume":"23","author":"E Mass","year":"2023","unstructured":"Mass, E., Nimmerjahn, F., Kierdorf, K. & Schlitzer, A. Tissue-specific macrophages: how they develop and choreograph tissue biology. Nat. Rev. Immunol. 23, 563\u2013579 (2023).","journal-title":"Nat. Rev. Immunol."},{"key":"2481_CR3","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1038\/nature12034","volume":"496","author":"TA Wynn","year":"2013","unstructured":"Wynn, T. A., Chawla, A. & Pollard, J. W. Macrophage biology in development, homeostasis and disease. Nature 496, 445\u2013455 (2013).","journal-title":"Nature"},{"key":"2481_CR4","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1016\/j.immuni.2013.04.004","volume":"38","author":"D Hashimoto","year":"2013","unstructured":"Hashimoto, D. et al. Tissue-resident macrophages self-maintain locally throughout adult life with minimal contribution from circulating monocytes. Immunity 38, 792\u2013804 (2013).","journal-title":"Immunity"},{"key":"2481_CR5","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1038\/nature13989","volume":"518","author":"E Gomez Perdiguero","year":"2015","unstructured":"Gomez Perdiguero, E. et al. Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors. Nature 518, 547\u2013551 (2015).","journal-title":"Nature"},{"key":"2481_CR6","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1016\/j.immuni.2015.03.011","volume":"42","author":"G Hoeffel","year":"2015","unstructured":"Hoeffel, G. et al. C-Myb(+) erythro-myeloid progenitor-derived fetal monocytes give rise to adult tissue-resident macrophages. Immunity 42, 665\u2013678 (2015).","journal-title":"Immunity"},{"key":"2481_CR7","doi-asserted-by":"publisher","first-page":"4147","DOI":"10.1242\/dev.012385","volume":"134","author":"JY Bertrand","year":"2007","unstructured":"Bertrand, J. Y. et al. Definitive hematopoiesis initiates through a committed erythromyeloid progenitor in the zebrafish embryo. Development 134, 4147\u20134156 (2007).","journal-title":"Development"},{"key":"2481_CR8","doi-asserted-by":"publisher","first-page":"9868","DOI":"10.3390\/ijms23179868","volume":"23","author":"A Elchaninov","year":"2022","unstructured":"Elchaninov, A., Vishnyakova, P., Menyailo, E., Sukhikh, G. & Fatkhudinov, T. An eye on Kupffer cells: development, phenotype and the macrophage niche. Int. J. Mol. Sci. 23, 9868 (2022).","journal-title":"Int. J. Mol. Sci."},{"key":"2481_CR9","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1111\/1348-0421.12228","volume":"59","author":"D Kurotaki","year":"2015","unstructured":"Kurotaki, D., Uede, T. & Tamura, T. Functions and development of red pulp macrophages. Microbiol. Immunol. 59, 55\u201362 (2015).","journal-title":"Microbiol. Immunol."},{"key":"2481_CR10","doi-asserted-by":"crossref","unstructured":"An, H. et al. Splenic red pulp macrophages eliminate the liver-resistant Streptococcus pneumoniae from the blood circulation of mice. Sci. Adv. 11, eadq6399 (2025).","DOI":"10.1126\/sciadv.adq6399"},{"key":"2481_CR11","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1126\/science.1142883","volume":"317","author":"C Auffray","year":"2007","unstructured":"Auffray, C. et al. Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior. Science 317, 666\u2013670 (2007).","journal-title":"Science"},{"key":"2481_CR12","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1006\/dbio.2002.0711","volume":"248","author":"ND Lawson","year":"2002","unstructured":"Lawson, N. D. & Weinstein, B. M. In vivo imaging of embryonic vascular development using transgenic zebrafish. Dev. Biol. 248, 307\u2013318 (2002).","journal-title":"Dev. Biol."},{"key":"2481_CR13","doi-asserted-by":"publisher","first-page":"3976","DOI":"10.1182\/blood-2006-05-024075","volume":"108","author":"SA Renshaw","year":"2006","unstructured":"Renshaw, S. A. et al. A transgenic zebrafish model of neutrophilic inflammation. Blood 108, 3976\u20133978 (2006).","journal-title":"Blood"},{"key":"2481_CR14","doi-asserted-by":"publisher","first-page":"e49","DOI":"10.1182\/blood-2010-10-314120","volume":"117","author":"F Ellett","year":"2011","unstructured":"Ellett, F., Pase, L., Hayman, J. W., Andrianopoulos, A. & Lieschke, G. J. mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish. Blood 117, e49\u2013e56 (2011).","journal-title":"Blood"},{"key":"2481_CR15","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1038\/ng.321","volume":"41","author":"BM Hogan","year":"2009","unstructured":"Hogan, B. M. et al. Ccbe1 is required for embryonic lymphangiogenesis and venous sprouting. Nat. Genet. 41, 396\u2013398 (2009).","journal-title":"Nat. Genet."},{"key":"2481_CR16","doi-asserted-by":"publisher","first-page":"1228","DOI":"10.1242\/dev.105031","volume":"141","author":"A van Impel","year":"2014","unstructured":"van Impel, A. et al. Divergence of zebrafish and mouse lymphatic cell fate specification pathways. Development 141, 1228\u20131238 (2014).","journal-title":"Development"},{"key":"2481_CR17","doi-asserted-by":"publisher","first-page":"4271","DOI":"10.1128\/IAI.00976-13","volume":"81","author":"TK Prajsnar","year":"2013","unstructured":"Prajsnar, T. K. et al. Zebrafish as a novel vertebrate model to dissect enterococcal pathogenesis. Infect. Immun. 81, 4271\u20134279 (2013).","journal-title":"Infect. Immun."},{"key":"2481_CR18","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1016\/j.immuni.2014.10.008","volume":"41","author":"S Nourshargh","year":"2014","unstructured":"Nourshargh, S. & Alon, R. Leukocyte migration into inflamed tissues. Immunity 41, 694\u2013707 (2014).","journal-title":"Immunity"},{"key":"2481_CR19","doi-asserted-by":"publisher","first-page":"11633","DOI":"10.1073\/pnas.0702449104","volume":"104","author":"H Kress","year":"2007","unstructured":"Kress, H. et al. Filopodia act as phagocytic tentacles and pull with discrete steps and a load-dependent velocity. Proc. Natl Acad. Sci. USA 104, 11633\u201311638 (2007).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2481_CR20","doi-asserted-by":"publisher","first-page":"15850","DOI":"10.1073\/pnas.1000494107","volume":"107","author":"G Lugo-Villarino","year":"2010","unstructured":"Lugo-Villarino, G. et al. Identification of dendritic antigen-presenting cells in the zebrafish. Proc. Natl Acad. Sci. USA 107, 15850\u201315855 (2010).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2481_CR21","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1006\/cimm.1999.1612","volume":"200","author":"H El Sherbini","year":"2000","unstructured":"El Sherbini, H. et al. Lectin ligands on human dendritic cells and identification of a peanut agglutinin positive subset in blood. Cell. Immunol. 200, 36\u201344 (2000).","journal-title":"Cell. Immunol."},{"key":"2481_CR22","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.devcel.2004.11.014","volume":"8","author":"J Rhodes","year":"2005","unstructured":"Rhodes, J. et al. Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish. Dev. Cell 8, 97\u2013108 (2005).","journal-title":"Dev. Cell"},{"key":"2481_CR23","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1038\/nri2024","volume":"7","author":"F Rosenbauer","year":"2007","unstructured":"Rosenbauer, F. & Tenen, D. G. Transcription factors in myeloid development: balancing differentiation with transformation. Nat. Rev. Immunol. 7, 105\u2013117 (2007).","journal-title":"Nat. Rev. Immunol."},{"key":"2481_CR24","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/S1074-7613(00)00016-9","volume":"13","author":"T Tamura","year":"2000","unstructured":"Tamura, T., Nagamura-Inoue, T., Shmeltzer, Z., Kuwata, T. & Ozato, K. ICSBP directs bipotential myeloid progenitor cells to differentiate into mature macrophages. Immunity 13, 155\u2013165 (2000).","journal-title":"Immunity"},{"key":"2481_CR25","doi-asserted-by":"publisher","first-page":"1359","DOI":"10.1182\/blood-2010-06-290700","volume":"117","author":"L Li","year":"2011","unstructured":"Li, L., Jin, H., Xu, J., Shi, Y. & Wen, Z. Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis. Blood 117, 1359\u20131369 (2011).","journal-title":"Blood"},{"key":"2481_CR26","doi-asserted-by":"publisher","first-page":"9658","DOI":"10.1039\/D1SC01059J","volume":"12","author":"X Lu","year":"2021","unstructured":"Lu, X. et al. Photocleavable proteins that undergo fast and efficient dissociation. Chem. Sci. 12, 9658\u20139672 (2021).","journal-title":"Chem. Sci."},{"key":"2481_CR27","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1038\/s41556-021-00664-3","volume":"23","author":"B Weijts","year":"2021","unstructured":"Weijts, B. et al. Endothelial struts enable the generation of large lumenized blood vessels de novo. Nat. Cell Biol. 23, 322\u2013329 (2021).","journal-title":"Nat. Cell Biol."},{"key":"2481_CR28","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1038\/nature08738","volume":"464","author":"JY Bertrand","year":"2010","unstructured":"Bertrand, J. Y. et al. Haematopoietic stem cells derive directly from aortic endothelium during development. Nature 464, 108\u2013111 (2010).","journal-title":"Nature"},{"key":"2481_CR29","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1038\/nature08761","volume":"464","author":"K Kissa","year":"2010","unstructured":"Kissa, K. & Herbomel, P. Blood stem cells emerge from aortic endothelium by a novel type of cell transition. Nature 464, 112\u2013115 (2010).","journal-title":"Nature"},{"key":"2481_CR30","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1038\/nature07619","volume":"457","author":"MJ Chen","year":"2009","unstructured":"Chen, M. J., Yokomizo, T., Zeigler, B. M., Dzierzak, E. & Speck, N. A. Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature 457, 887\u2013891 (2009).","journal-title":"Nature"},{"key":"2481_CR31","doi-asserted-by":"publisher","first-page":"2015","DOI":"10.1242\/dev.129.8.2015","volume":"129","author":"ML Kalev-Zylinska","year":"2002","unstructured":"Kalev-Zylinska, M. L. et al. Runx1 is required for zebrafish blood and vessel development and expression of a human RUNX1-CBF2T1 transgene advances a model for studies of leukemogenesis. Development 129, 2015\u20132030 (2002).","journal-title":"Development"},{"key":"2481_CR32","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1038\/nature08764","volume":"464","author":"J-C Boisset","year":"2010","unstructured":"Boisset, J.-C. et al. In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium. Nature 464, 116\u2013120 (2010).","journal-title":"Nature"},{"key":"2481_CR33","doi-asserted-by":"publisher","first-page":"3955","DOI":"10.1182\/blood-2010-02-266296","volume":"116","author":"KPA MacDonald","year":"2010","unstructured":"MacDonald, K. P. A. et al. An antibody against the colony-stimulating factor 1 receptor depletes the resident subset of monocytes and tissue- and tumor-associated macrophages but does not inhibit inflammation. Blood 116, 3955\u20133963 (2010).","journal-title":"Blood"},{"key":"2481_CR34","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1182\/blood.V99.1.111","volume":"99","author":"X-M Dai","year":"2002","unstructured":"Dai, X.-M. et al. Targeted disruption of the mouse colony-stimulating factor 1 receptor gene results in osteopetrosis, mononuclear phagocyte deficiency, increased primitive progenitor cell frequencies, and reproductive defects. Blood 99, 111\u2013120 (2002).","journal-title":"Blood"},{"key":"2481_CR35","doi-asserted-by":"publisher","first-page":"e53403","DOI":"10.7554\/eLife.53403","volume":"9","author":"LE Kuil","year":"2020","unstructured":"Kuil, L. E. et al. Zebrafish macrophage developmental arrest underlies depletion of microglia and reveals Csf1r-independent metaphocytes. eLife 9, e53403 (2020).","journal-title":"eLife"},{"key":"2481_CR36","doi-asserted-by":"publisher","first-page":"1413","DOI":"10.1126\/science.abo4837","volume":"377","author":"SJ Wattrus","year":"2022","unstructured":"Wattrus, S. J. et al. Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality. Science 377, 1413\u20131419 (2022).","journal-title":"Science"},{"key":"2481_CR37","doi-asserted-by":"publisher","first-page":"1050","DOI":"10.1242\/dev.119180","volume":"142","author":"E Butko","year":"2015","unstructured":"Butko, E. et al. Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo. Development 142, 1050\u20131061 (2015).","journal-title":"Development"},{"key":"2481_CR38","doi-asserted-by":"publisher","first-page":"e11813","DOI":"10.7554\/eLife.11813","volume":"5","author":"AV Gore","year":"2016","unstructured":"Gore, A. V. et al. Epigenetic regulation of hematopoiesis by DNA methylation. eLife 5, e11813 (2016).","journal-title":"eLife"},{"key":"2481_CR39","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1006\/dbio.1998.8887","volume":"197","author":"MA Thompson","year":"1998","unstructured":"Thompson, M. A. et al. The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis. Dev. Biol. 197, 248\u2013269 (1998).","journal-title":"Dev. Biol."},{"key":"2481_CR40","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/j.cell.2013.03.010","volume":"153","author":"LM Carlin","year":"2013","unstructured":"Carlin, L. M. et al. Nr4a1-dependent Ly6C(low) monocytes monitor endothelial cells and orchestrate their disposal. Cell 153, 362\u2013375 (2013).","journal-title":"Cell"},{"key":"2481_CR41","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1038\/s41586-025-09639-y","volume":"648","author":"KR Mesa","year":"2025","unstructured":"Mesa, K. R. et al. Niche-specific dermal macrophage loss promotes skin capillary ageing. Nature 648, 173\u2013181 (2025).","journal-title":"Nature"},{"key":"2481_CR42","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25590-8","volume":"12","author":"K Bisht","year":"2021","unstructured":"Bisht, K. et al. Capillary-associated microglia regulate vascular structure and function through PANX1\u2013P2RY12 coupling in mice. Nat. Commun. 12, 5289 (2021).","journal-title":"Nat. Commun."},{"key":"2481_CR43","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13812-z","volume":"10","author":"K Haruwaka","year":"2019","unstructured":"Haruwaka, K. et al. Dual microglia effects on blood brain barrier permeability induced by systemic inflammation. Nat. Commun. 10, 5816 (2019).","journal-title":"Nat. Commun."},{"key":"2481_CR44","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11053-8","volume":"10","author":"R Rojo","year":"2019","unstructured":"Rojo, R. et al. Deletion of a Csf1r enhancer selectively impacts CSF1R expression and development of tissue macrophage populations. Nat. Commun. 10, 3215 (2019).","journal-title":"Nat. Commun."},{"key":"2481_CR45","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1016\/j.celrep.2018.06.113","volume":"24","author":"N Oosterhof","year":"2018","unstructured":"Oosterhof, N. et al. Colony-stimulating factor 1 receptor (CSF1R) regulates microglia density and distribution, but not microglia differentiation in vivo. Cell Rep. 24, 1203\u20131217 (2018).","journal-title":"Cell Rep."},{"key":"2481_CR46","doi-asserted-by":"publisher","first-page":"1474","DOI":"10.1182\/bloodadvances.2021005459","volume":"6","author":"M Hason","year":"2022","unstructured":"Hason, M. et al. M-CSFR\/CSF1R signaling regulates myeloid fates in zebrafish via distinct action of its receptors and ligands. Blood Adv. 6, 1474\u20131488 (2022).","journal-title":"Blood Adv."},{"key":"2481_CR47","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1182\/blood-2002-02-0569","volume":"101","author":"RT Sasmono","year":"2003","unstructured":"Sasmono, R. T. et al. A macrophage colony-stimulating factor receptor-green fluorescent protein transgene is expressed throughout the mononuclear phagocyte system of the mouse. Blood 101, 1155\u20131163 (2003).","journal-title":"Blood"},{"key":"2481_CR48","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/nature14425","volume":"522","author":"J Nicenboim","year":"2015","unstructured":"Nicenboim, J. et al. Lymphatic vessels arise from specialized angioblasts within a venous niche. Nature 522, 56\u201361 (2015).","journal-title":"Nature"},{"key":"2481_CR49","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-51637-7","volume":"15","author":"AE Williamson","year":"2024","unstructured":"Williamson, A. E. et al. Discovery of an embryonically derived bipotent population of endothelial-macrophage progenitor cells in postnatal aorta. Nat. Commun. 15, 7097 (2024).","journal-title":"Nat. Commun."},{"key":"2481_CR50","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.devcel.2014.11.024","volume":"32","author":"Y Wakayama","year":"2015","unstructured":"Wakayama, Y., Fukuhara, S., Ando, K., Matsuda, M. & Mochizuki, N. Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Dev. Cell 32, 109\u2013122 (2015).","journal-title":"Dev. Cell"},{"key":"2481_CR51","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.ydbio.2012.03.021","volume":"366","author":"JJ Ganis","year":"2012","unstructured":"Ganis, J. J. et al. Zebrafish globin switching occurs in two developmental stages and is controlled by the LCR. Dev. Biol. 366, 185\u2013194 (2012).","journal-title":"Dev. Biol."},{"key":"2481_CR52","doi-asserted-by":"publisher","first-page":"2776","DOI":"10.1242\/dev.091876","volume":"140","author":"CSM Helker","year":"2013","unstructured":"Helker, C. S. M. et al. The zebrafish common cardinal veins develop by a novel mechanism: lumen ensheathment. Development 140, 2776\u20132786 (2013).","journal-title":"Development"},{"key":"2481_CR53","doi-asserted-by":"publisher","first-page":"3803","DOI":"10.1182\/blood-2005-01-0179","volume":"106","author":"H-F Lin","year":"2005","unstructured":"Lin, H.-F. et al. Analysis of thrombocyte development in CD41\u2013GFP transgenic zebrafish. Blood 106, 3803\u20133810 (2005).","journal-title":"Blood"},{"key":"2481_CR54","doi-asserted-by":"publisher","first-page":"e1003561","DOI":"10.1371\/journal.pgen.1003561","volume":"9","author":"LB Patterson","year":"2013","unstructured":"Patterson, L. B. & Parichy, D. M. Interactions with iridophores and the tissue environment required for patterning melanophores and xanthophores during zebrafish adult pigment stripe formation. PLoS Genet. 9, e1003561 (2013).","journal-title":"PLoS Genet."},{"key":"2481_CR55","doi-asserted-by":"publisher","first-page":"936","DOI":"10.1016\/j.ajhg.2019.03.010","volume":"104","author":"N Oosterhof","year":"2019","unstructured":"Oosterhof, N. et al. Homozygous mutations in CSF1R cause a pediatric-onset leukoencephalopathy and can result in congenital absence of microglia. Am. J. Hum. Genet. 104, 936\u2013947 (2019).","journal-title":"Am. J. Hum. Genet."},{"key":"2481_CR56","doi-asserted-by":"publisher","first-page":"3347","DOI":"10.1084\/jem.20170488","volume":"214","author":"Y Tian","year":"2017","unstructured":"Tian, Y. et al. The first wave of T lymphopoiesis in zebrafish arises from aorta endothelium independent of hematopoietic stem cells. J. Exp. Med. 214, 3347\u20133360 (2017).","journal-title":"J. Exp. Med."},{"key":"2481_CR57","doi-asserted-by":"publisher","first-page":"811","DOI":"10.1016\/j.ydbio.2007.01.033","volume":"304","author":"JM Davison","year":"2007","unstructured":"Davison, J. M. et al. Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish. Dev. Biol. 304, 811\u2013824 (2007).","journal-title":"Dev. Biol."},{"key":"2481_CR58","doi-asserted-by":"publisher","first-page":"4193","DOI":"10.1242\/dev.01970","volume":"132","author":"D Beis","year":"2005","unstructured":"Beis, D. et al. Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development. Development 132, 4193\u20134204 (2005).","journal-title":"Development"},{"key":"2481_CR59","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/j.devcel.2005.01.010","volume":"8","author":"M Gering","year":"2005","unstructured":"Gering, M. & Patient, R. Hedgehog signaling is required for adult blood stem cell formation in zebrafish embryos. Dev. Cell 8, 389\u2013400 (2005).","journal-title":"Dev. Cell"},{"key":"2481_CR60","first-page":"e53272","volume":"30","author":"L Zang","year":"2015","unstructured":"Zang, L., Shimada, Y., Nishimura, Y., Tanaka, T. & Nishimura, N. Repeated blood collection for blood tests in adult zebrafish. J. Vis. Exp. 30, e53272 (2015).","journal-title":"J. Vis. Exp."},{"key":"2481_CR61","doi-asserted-by":"publisher","DOI":"10.3791\/1645","author":"EK Pugach","year":"2009","unstructured":"Pugach, E. K., Li, P., White, R. & Zon, L. Retro-orbital injection in adult zebrafish. J. Vis. Exp. https:\/\/doi.org\/10.3791\/1645 (2009).","journal-title":"J. Vis. Exp."},{"key":"2481_CR62","first-page":"e52037","volume":"11","author":"SE Gombash Lampe","year":"2014","unstructured":"Gombash Lampe, S. E., Kaspar, B. K. & Foust, K. D. Intravenous injections in neonatal mice. J. Vis. Exp. 11, e52037 (2014).","journal-title":"J. Vis. Exp."},{"key":"2481_CR63","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.ydbio.2011.01.002","volume":"352","author":"CM Akitake","year":"2011","unstructured":"Akitake, C. M., Macurak, M., Halpern, M. E. & Goll, M. G. Transgenerational analysis of transcriptional silencing in zebrafish. Dev. Biol. 352, 191\u2013201 (2011).","journal-title":"Dev. Biol."},{"key":"2481_CR64","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1089\/zeb.2016.1406","volume":"14","author":"B Weijts","year":"2017","unstructured":"Weijts, B., Tkachenko, E., Traver, D. & Groisman, A. A four-well dish for high-resolution longitudinal imaging of the tail and posterior trunk of larval zebrafish. Zebrafish 14, 489\u2013491 (2017).","journal-title":"Zebrafish"},{"key":"2481_CR65","doi-asserted-by":"publisher","first-page":"2301","DOI":"10.1038\/nprot.2016.136","volume":"11","author":"S Tyanova","year":"2016","unstructured":"Tyanova, S., Temu, T. & Cox, J. The MaxQuant computational platform for mass spectrometry-based shotgun proteomics. Nat. Protoc. 11, 2301\u20132319 (2016).","journal-title":"Nat. Protoc."},{"key":"2481_CR66","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1016\/j.cels.2016.09.002","volume":"3","author":"MJ Muraro","year":"2016","unstructured":"Muraro, M. J. et al. A single-cell transcriptome atlas of the human pancreas. Cell Syst. 3, 385\u2013394 (2016).","journal-title":"Cell Syst."},{"key":"2481_CR67","doi-asserted-by":"publisher","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","volume":"184","author":"Y Hao","year":"2021","unstructured":"Hao, Y. et al. Integrated analysis of multimodal single-cell data. Cell 184, 3573\u20133587 (2021).","journal-title":"Cell"}],"container-title":["Nature Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41590-026-02481-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41590-026-02481-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41590-026-02481-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T19:02:43Z","timestamp":1777575763000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41590-026-02481-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,31]]},"references-count":67,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["2481"],"URL":"https:\/\/doi.org\/10.1038\/s41590-026-02481-y","relation":{},"ISSN":["1529-2908","1529-2916"],"issn-type":[{"value":"1529-2908","type":"print"},{"value":"1529-2916","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,31]]},"assertion":[{"value":"22 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 March 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}