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However, by using an intrinsic on\u2013off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells.<\/jats:p>","DOI":"10.1038\/s41592-022-01577-1","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T16:02:54Z","timestamp":1662048174000},"page":"1072-1075","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":134,"title":["DNA-PAINT MINFLUX nanoscopy"],"prefix":"10.1038","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7132-9813","authenticated-orcid":false,"given":"Lynn M.","family":"Ostersehlt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6356-9742","authenticated-orcid":false,"given":"Daniel C.","family":"Jans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7741-125X","authenticated-orcid":false,"given":"Anna","family":"Wittek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Keller-Findeisen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5959-6409","authenticated-orcid":false,"given":"Kaushik","family":"Inamdar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2917-0778","authenticated-orcid":false,"given":"Steffen J.","family":"Sahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9638-5077","authenticated-orcid":false,"given":"Stefan W.","family":"Hell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8028-3121","authenticated-orcid":false,"given":"Stefan","family":"Jakobs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,9,1]]},"reference":[{"key":"1577_CR1","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1038\/nmeth.1291","volume":"6","author":"SW Hell","year":"2009","unstructured":"Hell, S. 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All other authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}