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In particular, many red indicators are relatively dim, produce low signal-to-noise ratios and can undergo unwanted photoswitching when exposed to blue light, restricting their use in all-optical experiments that combine imaging with optogenetics or multicolor imaging. Here we show the development of PinkyCaMP, a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    sensor based on the bright red fluorescent protein mScarlet. PinkyCaMP exhibits markedly improved brightness, photostability and signal-to-noise ratio compared to existing red GECIs, while remaining fully compatible with blue-light-based optogenetic and dual-color imaging approaches. PinkyCaMP is well-tolerated by neurons, showing no detectable toxicity or aggregation, both in vitro and in vivo. PinkyCaMP enables a broad spectrum of imaging modalities, including single-photon methods, such as fiber photometry, widefield imaging and miniature microscopy imaging, as well as two-photon imaging in awake mice.\n                  <\/jats:p>","DOI":"10.1038\/s41592-026-03065-2","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T09:02:38Z","timestamp":1777021358000},"page":"998-1010","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing 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